Diversity of endophytic fungi in Glycine max

Fernandes, Elio Gomes; Pereira, Olinto Liparini; Silva, Cynthia Cânedo da; Bento, Cláudia Braga Pereira; Queiroz, Marisa Vieira de

doi:10.1016/j.micres.2015.05.010

Cited by 90 publications

(62 citation statements)

References 47 publications

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“…Similar studies have tested either a different medium or a range of media under different cultural conditions. Verma et al (2011) found that some of the endophytic isolates were only recovered on certain media, while other researchers have chosen a different culture medium than ours (de Souza Leite et al 2013; Fernandes et al 2015). Further experimental work with a range of culture media and cultural conditions is necessary to determine the full diversity of endophytes in our selected host.…”

Section: Discussionmentioning

confidence: 77%

Profundae diversitas: the uncharted genetic diversity in a newly studied group of fungal root endophytes

et al. 2015

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Endophytes associated with crops have potential as beneficial inoculants in agriculture, but little is known about their genetic diversity and phylogenetic relationships. We carried out the first ever ecological and phylogenetic survey of the culturable fungal root endophytes of a wild barley species. Fungal root endophytes were isolated from 10 populations of wall barley (Hordeum murinum), and 112 taxa of fungi were identified based on internal transcribed spacer sequence similarity. We found representatives from 8 orders, 12 families and 18 genera. Within this group, only 34 isolates (30% of the total) could be confidently assigned to a species, and 23 of the isolates (21% of the total) had no significant match to anything deposited in GenBank (based on <85% sequence similarity). These results suggest a high proportion of novel fungi, with 28% not assigned to a known fungal order. This includes three endophytes that have been shown to significantly improve agronomic traits in cultivated barley. This study has, therefore, revealed a profound diversity of fungal root endophytes in a single wild relative of barley. Extrapolating from this, the study highlights the largely unknown, hugely diverse and potentially useful resource of crop wild relative endophytes.

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Section: Discussionmentioning

confidence: 77%

Profundae diversitas: the uncharted genetic diversity in a newly studied group of fungal root endophytes

et al. 2015

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“…The crop has been known as a rich source of beneficial endophytic fungi improving its fitness to withstand environmental stresses including salinity (Pimentel et al, 2006; Khan et al, 2011). About 187 species of fungal endophytes have been reported from different parts of soybean (Fernandes et al, 2015) which belong to different taxonomic groups including Ascomycota and Basidiomycota (de Souza Leite et al, 2013). Soybean leaves were comparatively richer in fungal endophytes than its roots.…”

Section: Introductionmentioning

confidence: 99%

Gibberellins Producing Endophytic Fungus Porostereum spadiceum AGH786 Rescues Growth of Salt Affected Soybean

et al. 2017

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In the pursuit of sustainable agriculture through environment and human health friendly practices, we evaluated the potential of a novel gibberellins (GAs) producing basidiomycetous endophytic fungus Porostereum spadiceum AGH786, for alleviating salt stress and promoting health benefits of soybean. Soybean seedlings exposed to different levels of NaCl stress (70 and 140 mM) under greenhouse conditions, were inoculated with the AGH786 strain. Levels of phytohormones including GAs, JA and ABA, and isoflavones were compared in control and the inoculated seedlings to understand the mechanism through which the stress is alleviated. Gibberellins producing endophytic fungi have been vital for promoting plant growth under normal and stress conditions. We report P. spadiceum AGH786 as the ever first GAs producing basidiomycetous fungus capable of producing six types of GAs. In comparison to the so for most efficient GAs producing Gibberella fujikuroi, AGH786 produced significantly higher amount of the bioactive GA3. Salt-stressed phenotype of soybean seedlings was characterized by low content of GAs and high amount of ABA and JA with reduced shoot length, biomass, leaf area, chlorophyll contents, and rate of photosynthesis. Mitigation of salt stress by AGH786 was always accompanied by high GAs, and low ABA and JA, suggesting that this endophytic fungus reduces the effect of salinity by modulating endogenous phytohormones of the seedlings. Additionally, this strain also enhanced the endogenous level of two isoflavones including daidzen and genistein in soybean seedlings under normal as well as salt stress conditions as compared to their respective controls. P. spadiceum AGH786 boosted the NaCl stress tolerance and growth in soybean, by modulating seedlings endogenous phytohormones and isoflavones suggesting a valuable contribution of this potent fungal biofertilizer in sustainable agriculture in salt affected soils.

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“…Although, DSE studies have focused on descriptive analyses of the colonization and fungal strains, mostly isolated from natural ecosystems (Jumpponen & Trappe, 1998;Newsham, 2011), while, when considering crop species, the information related to their association with DSEs is scarce and limited (Wang et al, 2016;Yuan et al, 2010;Rothen et al, 2017;Fernandes et al, 2015;Muthukumar & Tamilselvi, 2010). Argentina produces approximately 2% of the global wheat production, with an average planting area of 6 million hectares in the last 5 years (Andrade & Satorre, 2015), thus becoming one of the most important crops in the country.…”

Section: Introductionmentioning

confidence: 99%

Xylariales): Physiological characterization and structural features of its association with wheat.

Rothen¹,

Miranda²,

Fracchia³

et al. 2018

Bol. Soc. Argent. Bot.

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Summary: Plant roots can be colonized by asymptomatic fungal strains belonging to several taxa, among them, the group defined as Dark Septate Endophytes (DSE). Microdochium bolleyi commonly colonizes wheat roots and other crops. It is considered a weak pathogen or even a non-pathogenic fungal species, which has also been considered as a potential biocontrol agent against aggressive soil-borne pathogens in cereal crops. We isolated a strain of M. bolleyi from wheat roots sampled in a crop field in Argentina, and characterized its abilities to grow in different carbon and nitrogen sources, to produce indole and to solubilize phosphorus; also several enzymatic activities were evaluated. In addition, resynthesis was performed under controlled conditions in order to characterize root fungal colonization under both, optical and transmission microscopy. The strain 22-1 colonized wheat root parenchymal tissue, forming chlamysdospores inside parenchymal cells and root hairs, and poorly grew in carbon and nitrogen sources. This fungus also synthesized indoles in in vitro culture, but it cannot solubilize phosphorus. Only amylase activity was detected out of seven enzymatic activity measured. Microdochium bolleyi (strain 22-1) colonized the roots, it formed typical DSE fungal structures and behaved like a "true endophyte"; however further studies are necessary to elucidate its role in the association with wheat.Key words: Dark septate endophytes, Microdochium bolleyi, root endophytes, wheat crop.Resumen: Microdochium bolleyi (Ascomycota: Xylariales): Caracterización fisiológica y caracteres estructurales de su asociación con trigo. Las raíces de las plantas hospedan una gran diversidad de hongos, entre ellos, se encuentran los Endofitos Septados Oscuros (ESO). Microdochium bolleyi coloniza las raíces de trigo y otros cereales, aunque algunos autores lo han considerado un patógeno débil, otros han demostrado su acción biocontroladora contra patógenos agresivos del suelo. En el presente trabajo, se aisló una cepa de M. bolleyi (22-1) de raíces de trigo. Esta cepa fue metabólicamente caracterizada y se realizó un ensayo de resíntesis bajo condiciones controladas con el fin de caracterizar la colonización del hongo en la raíz bajo microscopía óptica y de transmisión. Su crecimiento fue escaso en las fuentes de carbono y nitrógeno evaluadas, sintetizó indoles en cultivo in vitro, pero no mostró habilidades para solubilizar el fósforo, por último, solo se detectó actividad amilasa. La cepa 22-1 coloniza la corteza radicular del trigo, formando clamidosporas melanizadas inter e intracelularmente y en el interior de los pelos radiculares. Microdochium bolleyi (cepa 22-1) coloniza la raíz de trigo formando las típicas estructuras de los ESO y comportándose como un "verdadero endófito", sin embargo, se necesitan más estudios para terminar de dilucidar su papel en la asociación con el trigo.Palabras clave: Endofitos septados oscuros, hongos endofitos, Microdochium bolleyi, trigo.

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Diversity of endophytic fungi in Glycine max

Cited by 90 publications

References 47 publications

Profundae diversitas: the uncharted genetic diversity in a newly studied group of fungal root endophytes

Profundae diversitas: the uncharted genetic diversity in a newly studied group of fungal root endophytes

Gibberellins Producing Endophytic Fungus Porostereum spadiceum AGH786 Rescues Growth of Salt Affected Soybean

Xylariales): Physiological characterization and structural features of its association with wheat.

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