Diversity of endophytic fungi in Coptis chinensis Franch. and their activity against methicillin-resistant Staphylococcus aureus

Ming, Qianqian; Huang, Xinghua; Guo, Lei; Liu, Dong; Qin, Lingyue; He, Yimo; Tang, Yu; Liu, Yanxia; Liu, Junkang; Li, Peng

doi:10.1007/s12223-022-00994-1

Cited by 3 publications

(6 citation statements)

References 39 publications

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“…In contrast, only five fugal communities, i.e., Apiospora , Epicoccum , Phoma , Phyllosticta , and Talaromyces ) were found among the roots and leaves, and only two genera ( Aspergillus and Chaetomium ) were detected in all tissues. These results suggest that our reported endophytic fungal genera have obvious tissue specificity features to some extent, which similar to the previous studies ( Xing and Guo, 2011 ; D’Souza and Hiremath, 2013 ; Cui et al, 2015 ; Ming et al, 2022 ).…”

Section: Discussionsupporting

confidence: 92%

Cultivable endophytic fungal community associated with the karst endemic plant Nervilia fordii and their antimicrobial activity

et al. 2022

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Endophytic fungi from medicinal plants with specific pharmacological functions attract much attention to provide the possibility of discovering valuable natural drugs with novel structures and biological activities. Nervilia fordii is a rare and endangered karst endemic plant that is used as medicine and food homology in Guangxi, China. These plants have been reported to have antimicrobial, antitumor, antiviral, and anti-inflammatory activities. However, few studies have focused on the diversity and antibacterial activity of endophytic fungi from N. fordii. In the present study, 184 endophytic fungi were isolated from the healthy tissues of N. fordii, and their molecular diversity and antimicrobial activities were analyzed for the first time. These fungi were categorized into 85 different morphotypes based on the morphological characteristics and the similarity between the target sequence and the reference sequence in the GenBank database. With the exception of 18 unidentified fungi, the fungal isolates belonged to at least 2 phyla, 4 classes, 15 orders, 45 known genera, and 45 different species, which showed high abundance, rich diversity, and obvious tissue specificity. All isolates were employed to screen for their antimicrobial activities via the agar diffusion method against Escherichia coli, Staphylococcus aureus, and Candida tropicalis. Among these endophytes, eight strains (9.41%) displayed inhibitory activity against E. coli, 11 strains (12.94%) against S. aureus, and two strains (2.35%) against C. tropicalis, to some extent. In particular, our study showed for the first time that the fungal agar plugs of Penicillium macrosclerotiorum 1151# exhibited promising antibacterial activity against E. coli and S. aureus. Moreover, the ethyl acetate (EA) extract of P. macrosclerotiorum 1151# had antibacterial effects against E. coli and S. aureus with a minimum inhibitory concentration (MIC) of 0.5 mg ml–1. Further research also confirmed that one of the antimicrobial compounds of P. macrosclerotiorum 1151# was methyl chloroacetate and exhibited excellent antibacterial activity against E. coli and S. aureus up to 1.71-fold and 1.13-fold compared with tetracycline (TET) (5 mg ml–1), respectively. Taken together, the present data suggest that various endophytic fungi of N. fordii could be exploited as sources of novel natural antimicrobial agents.

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Section: Discussionsupporting

confidence: 92%

Cultivable endophytic fungal community associated with the karst endemic plant Nervilia fordii and their antimicrobial activity

et al. 2022

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“…The diversity of fungal endophytes was higher in leaf than that of barks and roots. This result was similar to the endophytic fungi diversity in Croton chinensis (43). Similar research was also observed in Dillenia indica where the frequency of endophytic fungi was showing higher in the leaf tissue (8).…”

Section: Discussionsupporting

confidence: 89%

“…It was clearly indicated that endophytic fungi favour colonizing the leaf tissues because of their higher surface area, rich nutrients and thin walls (42). In contrast to the rhizome and fibrous roots, recent investigations have shown that the leaves are more significant in holding the majority of endophytic fungus (43). In previous studies, the recovery of endophytic fungi from leaf tissue was higher than that of the stem and petiole tissue demonstrating that the characteristics of leaf tissues and the size of the surface area are widely open up to the environment, favour spore deposition and dispersion that may result in endophytes in due time and conditions (44).…”

Section: Discussionmentioning

confidence: 99%

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Endophytic fungal diversity in Terminalia arjuna (Roxb.) Wight & Arn. of Tripura, Northeast India at different sampling sites and plant organs

Tripura,

Debbarma,

Paul

et al. 2024

Plant Sci. Today

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Endophytic fungi are ubiquitous in plant kingdom and play a vital role in balancing the microenvironments within the host plants. Fungal endophytes isolated from ethno-botanically important plants were the source of several secondary metabolites with potential biological activities. The present study has documented the variability of culturable endophytic fungi from Terminalia arjuna (Roxb.) Wight & Arn. (Combretaceae), a widely used medicinal plant of Tripura, North-east, India. A total of 613 fungal strains were isolated from 720 tissue segments viz. leaves, bark and root of T. arjuna. The highest numbers of endophytic fungal isolates were found to be colonizing in leaves (257) followed by barks (182) and the least number of isolates were obtained from roots (174). The fungal isolates were classified into 27 individual fungal strains based on morphological and microscopic features. A total of 9 endophytic fungal strains were identified using the nuclear ribosomal DNA internal transcribe sequence and were subjected to phylogeny analysis. Most of the identified morphotypes belonged to phylum Ascomycota. Among all the isolates, Diaporthe sp., Fusarium sp., Colletotrichum sp., Penicillium sp. were the most abundant fungal isolates from T. arjuna. The fungal orders namely Sordariomycetes was the most prevalent followed by Eurotiomycetes and Dothideomycetes. Colonization rate, isolation rate, colonization frequency and relative frequency results suggest that leaf segments harbors maximum endophytic fungi assemblage compared to bark and root tissues. The analysis of Shannon (H'), Simpson (1-D), Fisher alpha (a) and Brillouin (HB) indices had significant difference with locations and tissue type. The host plant harbor unique endophytic community composition in studied sampling locations and their colonization varied between the inoculated vegetative parts of the plant. As no such study has been undertaken in the North- eastern part of India, this pioneer study may enable to unearth novel fungal endophytes that might have beneficial role in plant growth promotion, stress tolerance etc.

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“…Conidia produced by P. litseae are oblong to ellipsoidal and aseptate with two large polar guttules ( Jiang et al 2017 ). This species as an endophytic fungus in Coptis chinensis exhibited obvious inhibition against methicillin-resistant Staphylococcus aureus ( Ming et al 2022 ). In the present study, two strains were isolated from diseased tea plant leaves.…”

Section: Resultsmentioning

confidence: 99%

Didymellaceae species associated with tea plant (Camellia sinensis) in China

Wang,

Tu,

Chen

et al. 2024

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Tea plant is one of the most important commercial crops worldwide. The Didymellaceae fungi can cause leaf blight disease of tea plant. In this study, 240 isolates were isolated from tea plant leaves of 10 provinces in China. Combined with multi-locus (ITS, LSU, RPB2 and TUB2) phylogenetic analysis and morphological characteristics, these isolates were identified as 25 species of six genera in Didymellaceae, including 19 known species Didymella coffeae-arabicae, D. pomorum, D. segeticola, D. sinensis, Epicoccum catenisporum, E. dendrobii, E. draconis, E. italicum, E. latusicollum, E. mackenziei, E. oryzae, E. poaceicola, E. rosae, E. sorghinum, E. tobaicum, Neoascochyta mortariensis, Paraboeremia litseae, Remotididymella anemophila and Stagonosporopsis caricae, of which 15 species were new record species and six novel species, named D. yunnanensis, E. anhuiense, E. jingdongense, E. puerense, N. yunnanensis and N. zhejiangensis. Amongst all isolates, D. segeticola was the most dominant species. Pathogenicity tests on tea plant leaves showed that E. anhuiense had the strongest virulence, while E. puerense had the weakest virulence. Besides, D. pomorum, D. yunnanensis, E. dendrobii, E. italicum, E. jingdongense, E. mackenziei, E. oryzae, E. rosae, E. tobaicum, N. mortariensis, N. yunnanensis, N. zhejiangensis and R. anemophila were non-pathogenic to the tea plant.

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Diversity of endophytic fungi in Coptis chinensis Franch. and their activity against methicillin-resistant Staphylococcus aureus

Cited by 3 publications

References 39 publications

Cultivable endophytic fungal community associated with the karst endemic plant Nervilia fordii and their antimicrobial activity

Cultivable endophytic fungal community associated with the karst endemic plant Nervilia fordii and their antimicrobial activity

Endophytic fungal diversity in Terminalia arjuna (Roxb.) Wight & Arn. of Tripura, Northeast India at different sampling sites and plant organs

Didymellaceae species associated with tea plant (Camellia sinensis) in China

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