Abstract:Background:Nepenthes species are used in traditional medicines to treat various health ailments. However, we do not know which types of endophytic bacteria (EB) are associated with Nepenthes spp.Objective:The objective of this study was to isolate and to identify EB associated with Nepenthes spp.Materials and Methods:Surface-sterilized leaf and stem tissues from nine Nepenthes spp. collected from Peninsular Malaysia were used to isolate EB. Isolates were identified using the polymerase chain reaction-amplified… Show more
“…Guanine and cytosine base pairs dominated the nucleotide base composition of the 16S rRNA gene in the HR11082 bacterial isolate. The bond between guanine nucleotide bases and G-C is strong because it has three hydrogen bonds, whereas the bond between A-T nucleotide bases is weak because it only has two hydrogen bonds [9]; [4]. Variations in nucleotide bases of bacterial isolate HR11082 with 16S rRNA sequences discovered more transition types than transversion types, which could be due to transversion having a higher chance of 1/140, while transitions had a 1/58 chance between the four nucleotide bases.…”
Section: Resultsmentioning
confidence: 99%
“…Nepenthes is useful as an insect biological control agent because it provides nectar to insects [3]. Nepenthes has a symbiotic relationship with chitinolytic bacteria found in Nepenthes cotton fluid, and these bacteria can be used as potential and environmentally friendly biological controllers to deal with pests and pathogens, particularly insects, in cultivated plants [4].…”
Nepenthes is a protected and unique carnivorous plant because it has a fluid-filled pouch at the end of the leaf that serves as an insect trap. The existence of Nepenthes gracillis in ecosystems is endangered, one of the reasons is caused by the attack of pathogenic bacteria, so it is necessary to identify pathogenic bacteria that attack N. gracillis as an effort to handle. The purpose of this study was to identify pathogenic bacteria in N. gracillis using 16S rRNA gene sequencing results. This study aims to identify the 16S rRNA sequence and phylogenetic of bacteria attached in N. Gracillis leaves, and the identification was confirmed using molecular data. The construction of phylogenetic topology was made based on ML and NJ method using Kimura-2 parameter model. Subsequently, molecular characterization and identification was conducted based on 720 bp of 16S rRNA gene similarity, sequence variation, genetic distance, and phylogenetic topology. According to the results, the bacteria species attached in N. Gracillis identified as Lelliottia nimipressuralis, and closely related with Erwinia aphidicola. Genetic distance between sample and related species was 0,0173, with 98,26% similarities, then strongly 100 bootstrap analyses. Thus, this study provides more information for Lelliottia nimipressuralis phylogenetic studies using different marker.
“…Guanine and cytosine base pairs dominated the nucleotide base composition of the 16S rRNA gene in the HR11082 bacterial isolate. The bond between guanine nucleotide bases and G-C is strong because it has three hydrogen bonds, whereas the bond between A-T nucleotide bases is weak because it only has two hydrogen bonds [9]; [4]. Variations in nucleotide bases of bacterial isolate HR11082 with 16S rRNA sequences discovered more transition types than transversion types, which could be due to transversion having a higher chance of 1/140, while transitions had a 1/58 chance between the four nucleotide bases.…”
Section: Resultsmentioning
confidence: 99%
“…Nepenthes is useful as an insect biological control agent because it provides nectar to insects [3]. Nepenthes has a symbiotic relationship with chitinolytic bacteria found in Nepenthes cotton fluid, and these bacteria can be used as potential and environmentally friendly biological controllers to deal with pests and pathogens, particularly insects, in cultivated plants [4].…”
Nepenthes is a protected and unique carnivorous plant because it has a fluid-filled pouch at the end of the leaf that serves as an insect trap. The existence of Nepenthes gracillis in ecosystems is endangered, one of the reasons is caused by the attack of pathogenic bacteria, so it is necessary to identify pathogenic bacteria that attack N. gracillis as an effort to handle. The purpose of this study was to identify pathogenic bacteria in N. gracillis using 16S rRNA gene sequencing results. This study aims to identify the 16S rRNA sequence and phylogenetic of bacteria attached in N. Gracillis leaves, and the identification was confirmed using molecular data. The construction of phylogenetic topology was made based on ML and NJ method using Kimura-2 parameter model. Subsequently, molecular characterization and identification was conducted based on 720 bp of 16S rRNA gene similarity, sequence variation, genetic distance, and phylogenetic topology. According to the results, the bacteria species attached in N. Gracillis identified as Lelliottia nimipressuralis, and closely related with Erwinia aphidicola. Genetic distance between sample and related species was 0,0173, with 98,26% similarities, then strongly 100 bootstrap analyses. Thus, this study provides more information for Lelliottia nimipressuralis phylogenetic studies using different marker.
“…The presence and absence of different chemicals in surface sterilization protocol were accounted as 1 and 0, respectively. The data obtained were subjected to analysis using Displayr software and a heat map was generated Bhore et al [106] for isolation of endophytes from Nepenthes species, 70% ethanol was used as surface sterilant [106]. Similarly, Schulz et al [73] used 50% ethanol for 5 min to surface sterilize plants like Anemone nemorosa, Ranunculus ficaria, Vaccinium oxycoccus, and Sambucus nigra.…”
Section: Surface Sterilization Using Single Sterilantmentioning
Endophytic microbiota opens a magnificent arena of metabolites that served as a potential source of medicines for treating a variety of ailments and having prospective uses in agriculture, food, cosmetics, and many more. There are umpteen reports of endophytes improving the growth and tolerance of plants. In addition, endophytes from lifesaving drug-producing plants such as Taxus, Nothapodytes, Catharanthus, and so forth have the ability to produce host mimicking compounds. To harness these benefits, it is imperative to isolate the true endophytes, not the surface microflora. The foremost step in endophyte
“…Meskipun demikian, sejumlah komunitas bakteri, seperti kelompok Alfaproteobakteria, dilaporkan mampu hidup dengan baik di dalamnya (Chou et al 2014). Selain cairan dalam kantung, bagian lain dari tanaman kantung semar juga dilaporkan merupakan habitat bagi sejumlah bakteri yang bersifat endofit (Bhore et al 2013). Adanya bakteri-bakteri tersebut diduga membantu tanaman dalam mencerna serangga.…”
Section: Hasil Dan Pembahasan Isolasi Bakteri Dari Cairan Kantung Semarunclassified
The genus Fusarium sp. is a pathogenic fungal for many cultivated plants. The bacteria isolated from monkey cup (Nepenthes sp.) fluid possess the ability to produce hydrolytic enzyme, such as chitinase which can be utilized to inhibit the growth of mycelia of pathogenic fungi. The aims of this study are to isolate bacteria from monkey cup liquid, to test their abilities to produce protease, chitinase, and cellulase, as well as their abilities to inhibit Fusarium. The bacteria were isolated using serial dilution method on Reasoner’s 2A agar medium. Enzymatic activities of bacterial isolates were determined by inoculating them on tested medium supplemented with casein protein, chitin, and cellulose, whereas their antifungal activities were assayed using a direct confrontation method between tested bacterial isolates and pathogenic fungal on Malt Extract Agar medium. Molecular identification of bacteria with antifungal activity was performed by analyzing the 16S rRNA gene sequences. Isolation process of bacteria from monkey cup fluid resulted in 99 bacterial isolates with the ability to produce either protease, chitinase, and/or cellulose enzymes. A total of 37 bacterial isolates were capable of producing at least two hydrolytic enzymes. Antifungal assay of those bacteria showed that as many as 25 isolates have the ability to inhibit the growth of Fusarium sp. Based on the analysis of 16S rRNA gene sequences revealed that those isolates were closely related to three Burkholderia species, namely B. arboris, B. contaminans, and B. rijonensis.
Keywords: antifungal, Burkholderia, chitinase, cellulaseN, epenthes, protease
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