Diversity of endophytic bacteria and fungi in seeds of Elymus nutans growing in four locations of Qinghai Tibet Plateau, China

Guo, Jianxiu; Bowatte, Saman; Hou, Fujiang

doi:10.1007/s11104-020-04608-y

Cited by 21 publications

(20 citation statements)

References 78 publications

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“…In agreement with this, bacterial communities in L. multiflorum seeds were dominated by members from the Proteobacteria, including the above-mentioned genera and a few other groups (e.g., Erwinia spp.). This pattern of bacterial diversity in L. multiflorum seeds is similar to those documented in seeds from other grass species such as Lolium perenne ( Tannenbaum et al, 2020 , 2021 ), Elymus nutans ( Guo et al, 2021 ), Oryza sativa ( Eyre et al, 2019 ; Wang et al, 2020a ) and Triticum aestivum ( Hone et al, 2021 ; Walsh et al, 2021 ). Several beneficial bacteria within the Pantoea and Pseudomonas genera have been isolated from plant seeds ( Feng et al, 2006 ; Díaz Herrera et al, 2016 ; Verma et al, 2018 ).…”

Section: Discussionsupporting

confidence: 79%

“…In the present study, Epichloë endophyte presence was associated with increased diversity and changes on composition of seed-associated bacterial communities, particularly in members from Pantoea and Pseudomonas . Interestingly, both genera contain species/strains described to provide a suite of benefits to plants such as growth promotion, nitrogen fixation, phosphate solubilisation and protection against pathogens/plant competitors ( Elmore et al, 2019 ; Guo et al, 2021 ). For instance, several plant-growth promoting Pseudomonas isolates increased the seed germination and seedling growth of Solanum lycopersicum plants (through production of auxins, phosphate-solubilising compounds and another growth-promoting compounds; Qessaoui et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

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Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities

et al. 2022

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Seeds commonly harbour diverse bacterial communities that can enhance the fitness of future plants. The bacterial microbiota associated with mother plant’s foliar tissues is one of the main sources of bacteria for seeds. Therefore, any ecological factor influencing the mother plant’s microbiota may also affect the diversity of the seed’s bacterial community. Grasses form associations with beneficial vertically transmitted fungal endophytes of genus Epichloë. The interaction of plants with Epichloë endophytes and insect herbivores can influence the plant foliar microbiota. However, it is unknown whether these interactions (alone or in concert) can affect the assembly of bacterial communities in the produced seed. We subjected Lolium multiflorum plants with and without its common endophyte Epichloë occultans (E+, E-, respectively) to an herbivory treatment with Rhopalosiphum padi aphids and assessed the diversity and composition of the bacterial communities in the produced seed. The presence of Epichloë endophytes influenced the seed bacterial microbiota by increasing the diversity and affecting the composition of the communities. The relative abundances of the bacterial taxa were more similarly distributed in communities associated with E+ than E- seeds with the latter being dominated by just a few bacterial groups. Contrary to our expectations, seed bacterial communities were not affected by the aphid herbivory experienced by mother plants. We speculate that the enhanced seed/seedling performance documented for Epichloë-host associations may be explained, at least in part, by the Epichloë-mediated increment in the seed-bacterial diversity, and that this phenomenon may be applicable to other plant-endophyte associations.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities

et al. 2022

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“…In our study, Ascomycota was a dominant and widespread phylum in all seed-borne fungal communities of different oat seeds. Our results demonstrated the earlier findings on Phragmites australis (Ernst et al, 2003), Elymus nutans (Guo et al, 2020), and Phragmites australis (Kalu et al, 2021), which showed this phylum as a common and dominant phylum. A total of 10 most abundant genera in different oat seeds were Alternaria, Mycosphaerella, Epicoccum, Cladosporium, Pyrenophora, Fusarium, Bipolaris, Guehomyces, Aspergillus, Stemphylium, and Nigrospora, respectively.…”

Section: Seed-borne Fungal Community Compositionsupporting

confidence: 91%

“…Therefore, these fungal species have positive and negative influences on host seeds. In nature, microbial communities are established in specific niches through mutualistic (positive), competitive (negative) and commensalistic (neutral) networks among different microbial groups (Faust and Raes, 2012;Guo et al, 2020). In this study, the interaction patterns of seedborne fungal genera in oat were also analyzed by using network analysis.…”

Section: The Interaction Patterns Of Seed-borne Fungal Genera In Oatmentioning

confidence: 99%

“…The fungal community network in oat seeds is controlled by a large number of taxa, which is also closely interconnected to have effects on the overall fungal community. These fungi also play key roles in oat seed survival and establishment of the next generation's seed-borne fungal community (Han et al, 2016;Guo et al, 2020).…”

Section: The Interaction Patterns Of Seed-borne Fungal Genera In Oatmentioning

confidence: 99%

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Effects of Oat Varieties and Growing Locations on Seed-Borne Fungal Communities

et al. 2021

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Many species of seed-borne fungi are closely allied with seed varieties and growing regions, including many seed-borne pathogens, but their species richness and distribution remain largely unknown. This study was conducted to explore the seed-borne fungal composition, abundance and diversity in Avena sativa (B7) and A. nuda (B2) seed samples collected from Baicheng (BB), Dingxi (DB) and Haibei (HB) city, using Illumina sequencing techniques. Our results show that a total of 543,707 sequences were obtained and these were assigned to 244 operational taxonomic units (OTUs) with 97% similarity. Oat varieties and growing locations had a significant difference on seed-borne fungal diversity. HB had a higher fungal diversity than BB and DB, Shannon diversity and ACE richness index of fungal in HB seeds was significantly higher than in BB and DB (P < 0.05). In different varieties, both taxon richness and evenness of B7 seeds was significantly higher than B2 (P < 0.05). A total of 4 fungal phyla and 26 fungal genera were detected. Ascomycota was the dominant phylum and Alternaria sp. was the most abundant genus in B2 and B7 oat seeds from different regions. Mycosphaerella sp. had a higher abundance in HB7 and DB7, respectively, Epicoccum sp. had a higher abundance in HB7 and BB7. The results of alpha and beta diversity analysis revealed the presence of different effects in fungal communities of different varieties and regions of oat, especially in seed pathogenic fungi distribution. Structural equation modeling also explained oat varieties and growing regions have significant influences on seed-borne fungal abundance, composition and diversity. This study demonstrated that the differences of varieties and regions are the main factors resulting in the changes of seed-borne fungal community of oat.

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Dynamic Changes of Soil Microbial Communities During the Afforestation of Pinus Armandii in a Karst Region of Southwest China

He,

Li,

Zou

et al. 2024

Microb Ecol

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Clarifying the response of soil microbial communities to vegetation restoration is essential to comprehend biogeochemical processes and ensure the long-term viability of forest development. To assess the variations in soil microbial communities throughout the growth of Pinus armandii plantations in the karst region, we utilized the “space instead of time” approach and selected four P. armandii stands with ages ranging from 10 to 47 years, along with a grassland control. The microbial community structure was determined by conducting Illumina sequencing of the 16 S rRNA gene and the ITS gene, respectively. The results demonstrated that afforestation with P. armandii significantly influenced soil microbial communities, as indicated by notable differences in bacterial and fungal composition and diversity between the plantations and the control. However, soil microbe diversity did not display significant variation across stand ages. Moreover, the bacterial community exhibited higher responsiveness to age gradients compared to the fungal community. Soil physicochemical factors play a critical role in elucidating microbial diversity and community composition variations during restoration processes. TN, AN, TP, AP, SOC, AK, and pH were the most significant influencing factors for the composition of bacterial community, while TC, SOC, pH, and TCa were the most significant influencing factors for the composition of fungal community. Our findings indicate substantial changes in soil bacterial and fungal communities across successive stages of development. Additionally, the changes in dominant bacteria and fungi characteristics across the age gradient were primarily attributed to variations in the prevailing soil conditions and chemical factors.

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Diversity of endophytic bacteria and fungi in seeds of Elymus nutans growing in four locations of Qinghai Tibet Plateau, China

Cited by 21 publications

References 78 publications

Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities

Epichloë Fungal Endophytes Influence Seed-Associated Bacterial Communities

Effects of Oat Varieties and Growing Locations on Seed-Borne Fungal Communities

Dynamic Changes of Soil Microbial Communities During the Afforestation of Pinus Armandii in a Karst Region of Southwest China

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