Diversity of dimethylsulfide‐degrading methanogens and sulfate‐reducing bacteria in anoxic sediments along the Medway Estuary, <scp>UK</scp>

Tsola, Stephania L.; Zhu, Yi‐Zhun; Ghurnee, Oshin; Economou, Chloe K.; Trimmer, Mark; Eyice, Özge

doi:10.1111/1462-2920.15637

Cited by 9 publications

(8 citation statements)

References 96 publications

(130 reference statements)

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“…Methanolobus are known DMS degraders with several strains isolated from an oil well, marine, lake and estuarine sediments 10,11 . We also recently showed Methanolobus to be the dominant DMS-degrading methanogen genus in brackish sediments from the Medway Estuary, UK 8 . Furthermore, a psychrotolerant Methanolobus strain has been isolated from a saline lake sediment in Siberia, indicating that this genus has members that can grow in low temperatures, as were measured in Baltic Sea sediments 56 .…”

Section: Discussionmentioning

confidence: 71%

“…Intriguingly, the sulfate concentrations in the sediments below 9 cm from all three sites increased significantly compared to the initial concentrations (Supplementary Figure 3). This suggests that hydrogen sulfide produced as one of the end products of DMS degradation was converted to sulfate, which led to cryptic sulfur cycling in these incubations 8 .…”

Section: Sediment Depth Profiles Of Dms Methane and Co2mentioning

confidence: 98%

“…In anoxic sediments, DMS can be degraded to potent greenhouse gases methane and carbon dioxide (CO2) by methylotrophic methanogens, further highlighting the significance of DMS 8 . Cultivation-based studies on DMS-dependent methanogenesis showed that this process is carried out by certain methanogens of the genera Methanomethylovorans, Methanolobus, Methanosarcina and Methanohalophilus [9][10][11][12] .…”

Section: Introductionmentioning

confidence: 99%

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Methanolobususe unspecific methyltransferases to produce methane from dimethylsulfide

Tsola

Chen

Sanders

et al. 2023

Preprint

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Dimethylsulfide (DMS) is the most abundant biogenic organic sulfur compound and a methane precursor in anoxic sediments. However, understanding of the microbial diversity driving DMS-dependent methanogenesis is limited, and the metabolic pathways underlying this process in the environment remain unexplored. To address this, we used anoxic incubations, amplicon sequencing, genome-centric metagenomics and metatranscriptomics of brackish sediments of the Baltic Sea. We identified Methanolobus as the dominant methylotrophic methanogens in all our sediment samples. We also showed that Methanolobus use trimethylamine- and methanol-methyltransferases, not methyl-sulfide methyltransferases, when producing methane from DMS. This demonstrated that methylotrophic methanogenesis does not require a substrate-specific methyltransferase as was previously accepted and highlights the versatility of the key enzymes in methane production in anoxic sediments.

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Section: Discussionmentioning

confidence: 71%

Section: Sediment Depth Profiles Of Dms Methane and Co2mentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Methanolobususe unspecific methyltransferases to produce methane from dimethylsulfide

Tsola

Chen

Sanders

et al. 2023

Preprint

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“…In anoxic sediments, DMS can be degraded to potent greenhouse gases methane and carbon dioxide (CO 2 ) by methylotrophic methanogens, further highlighting the significance of DMS [ 8 ]. Cultivation-based studies on DMS-dependent methanogenesis showed that this process is carried out by certain methanogens of the genera Methanomethylovorans , Methanolobus , Methanosarcina and Methanohalophilus [ 9 – 12 ].…”

Section: Introductionmentioning

confidence: 99%

Methanolobus use unspecific methyltransferases to produce methane from dimethylsulphide in Baltic Sea sediments

Tsola,

Zhu,

Chen

et al. 2024

Microbiome

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Background In anoxic coastal and marine sediments, degradation of methylated compounds is the major route to the production of methane, a powerful greenhouse gas. Dimethylsulphide (DMS) is the most abundant biogenic organic sulphur compound in the environment and an abundant methylated compound leading to methane production in anoxic sediments. However, understanding of the microbial diversity driving DMS-dependent methanogenesis is limited, and the metabolic pathways underlying this process in the environment remain unexplored. To address this, we used anoxic incubations, amplicon sequencing, genome-centric metagenomics and metatranscriptomics of brackish sediments collected along the depth profile of the Baltic Sea with varying sulphate concentrations. Results We identified Methanolobus as the dominant methylotrophic methanogens in all our DMS-amended sediment incubations (61–99%) regardless of their sulphate concentrations. We also showed that the mtt and mta genes (trimethylamine- and methanol-methyltransferases) from Methanolobus were highly expressed when the sediment samples were incubated with DMS. Furthermore, we did not find mtsA and mtsB (methylsulphide-methyltransferases) in metatranscriptomes, metagenomes or in the Methanolobus MAGs, whilst mtsD and mtsF were found 2–3 orders of magnitude lower in selected samples. Conclusions Our study demonstrated that the Methanolobus genus is likely the key player in anaerobic DMS degradation in brackish Baltic Sea sediments. This is also the first study analysing the metabolic pathways of anaerobic DMS degradation in the environment and showing that methylotrophic methane production from DMS may not require a substrate-specific methyltransferase as was previously accepted. This highlights the versatility of the key enzymes in methane production in anoxic sediments, which would have significant implications for the global greenhouse gas budget and the methane cycle.

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“…Increased sulfate availability caused by, for example, intrusion of sulfate-laden seawater to estuarine wetlands ( 10 ), will likely enable SRB to produce more H 2 S, utilizing H 2 and/or organic acids such as acetate and lactate as electron donors. The competition from SRB for H 2 and acetate may reduce methane production by hydrogenotrophic and acetoclastic methanogens ( 7 , 11 ). Characterization of these microbial interactions will provide insights into community metabolism under changing environmental conditions and allow modeling of metabolic flux rates among community members.…”

Section: Introductionmentioning

confidence: 99%

Cross-Feedings, Competition, and Positive and Negative Synergies in a Four-Species Synthetic Community for Anaerobic Degradation of Cellulose to Methane

Wang

Hunt

Candry

et al. 2023

mBio

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A synthetic community was designed using four microbial species that together performed distinct key metabolic processes in the anaerobic degradation of cellulose to methane and CO 2 . The microorganisms exhibited expected interactions, such as cross-feeding of acetate from a cellulolytic bacterium to an acetoclastic methanogen and competition of H 2 between a sulfate reducing bacterium and a hydrogenotrophic methanogen.

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Diversity of dimethylsulfide‐degrading methanogens and sulfate‐reducing bacteria in anoxic sediments along the Medway Estuary, UK

Cited by 9 publications

References 96 publications

Methanolobususe unspecific methyltransferases to produce methane from dimethylsulfide

Methanolobususe unspecific methyltransferases to produce methane from dimethylsulfide

Methanolobus use unspecific methyltransferases to produce methane from dimethylsulphide in Baltic Sea sediments

Cross-Feedings, Competition, and Positive and Negative Synergies in a Four-Species Synthetic Community for Anaerobic Degradation of Cellulose to Methane

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