Diversity of cultivable vaginal microbiota in asymptomatic women of reproductive age in Mumbai, India

Pramanick, Rinku; Parab, Shraddha; Mayadeo, Niranjan; Warke, Himangi; Aranha, Clara

doi:10.24870/cjb.2017-a115

Cited by 4 publications

(3 citation statements)

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“…This is the first report of its type from Northeast India and has implications for global comparison of Lactobacilli composition. Clearly, vaginal Lactobacillus composition among Northeast India population is different from other parts of India [22–24] and various regions/countries of the World [16–21]. Lactobacillus mucosae has been reported as less common vaginal microflora in previous studies [41, 48], however dominance of L. mucosae than L. crispatus and L. jensenii (as observed in most of the previous studies [2, 16]) observed in this study in Northeast Indian females is an intriguing finding and tentatively reflects different vaginal set-up.…”

Section: Discussionmentioning

confidence: 99%

“…A comparative study on Korean and Uganda women reported that L. fermentum was common in Korean, and L. gasseri, L. reuteri, and L. vaginalis in Ugandan women [21]. In India the biogeographical condition and population varies across different regions and as such the vaginal Lactobacillus species L. iners , L. crispatus , L. reuteri , L. gasseri , and L. jensenii have been found predominant in Mumbai (southwest region of India) [22], L. reuteri, L. fermentum, and L. salivarius in Delhi (central region of India) [23], L. crispatus, L. gasseri, and L. jenseni in Mysore (southern region of India) [24] and many of the regions still remain unexplored. A consensus of all studies explains four Lactobacillus species viz., L. crispatus, L. jenseni, L. gasseri, and L. iners [2] are predominant in vagina and among them L. crispatus and L. jenseni are considered most beneficial [25, 26] and their colonization corresponds to higher vaginal glycogen content [2].…”

Section: Introductionmentioning

confidence: 99%

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Contrasting diversity of vaginal lactobacilli among the females of Northeast India

et al. 2019

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Background Lactobacilli are gatekeepers of vaginal ecosystem impeding growth of pathogenic microbes and their diversity varies across populations worldwide. The present study investigated diversity of human vaginal microbiota among females of Northeast India, who are distinct in dietary habits, lifestyle, and genomic composition from rest of India. Results Altogether, 154 bacterial isolates were obtained from vaginal swab samples of 40 pregnant and 29 non-pregnant females. The samples were sequenced for 16 s rRNA gene and analysed for identification using a dual approach of homology search and maximum likelihood based clustering. Molecular identification based on 16S rRNA gene sequence confirmed the isolates belonging to 31 species. Lactobacilli constituted 37.7% of the bacterial isolates with 10 species and other Lactic Acid Bacteria (39.61%) represented another 10 species, some of which are opportunistic pathogens. The remaining of the communities are mostly dominated by species of Staphylococcus (14.28%) and rarely by Propionibacterium avidum (3.90%) , Bacillus subtilis, Escherchia coli, Janthinobacterium lividum, and Kocuria kristinae (each 0.64%). Interestingly Lactobacillus mucosae and Enterococcus faecalis, which are globally uncommon vaginal microbes is found dominant among women of Northeast India. This tentatively reflects adaptability of particular Lactobacillus species, in distinct population, to better compete for receptors and nutrients in vaginal epithelium than other species. Further, intrageneric 16S rRNA gene exchange was observed among Enterococcus, Staphylococcus , and two species of Lactobacillus , and deep intraspecies divergence among L. mucosae, which pinpointed possibility of emergence of new strains with evolved functionality. Lactobacilli percentage decreased from young pregnant to aged non-pregnant women with maximum colonization in trimester II. Conclusion The study highlighted importance of assessment of vaginal microbiota, Lactobacillus in particular, across different population to gain more insight on female health. Electronic supplementary material The online version of this article (10.1186/s12866-019-1568-6) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Contrasting diversity of vaginal lactobacilli among the females of Northeast India

et al. 2019

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“…Among the Japanese communities with Lactobacillus prevalence, L. crispatus was the predominant species, followed by L. iners, similar to Caucasian and African Americans in North America (Zhou et al, 2010). In India, several studies reported a geographic variation in vaginal microbiomes (Garg et al, 2009;Pramanick et al, 2017;Das Purkayastha et al, 2019): L. jensenii in the southwest region; L. reuteri, L. fermentum, and L. salivarius in the central region; and L. crispatus, L. gasseri, and L. jensenii in south India. Interestingly, the rare vaginal microbes L. mucosae and Enterococcus faecalis was found to be prevalent in Northeast India (Das Purkayastha et al, 2019).…”

Section: Vaginal Fluidmentioning

confidence: 85%

Forensic Analysis of Human Microbiome in Skin and Body Fluids Based on Geographic Location

Cho

Eom

2021

Front. Cell. Infect. Microbiol.

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High-throughput DNA sequencing technologies have facilitated the in silico forensic analysis of human microbiome. Specific microbial species or communities obtained from the crime scene provide evidence of human contacts and their body fluids. The microbial community is influenced by geographic, ethnic, lifestyle, and environmental factors such as urbanization. An understanding of the effects of these external stressors on the human microbiome and determination of stable and changing elements are important in selecting appropriate targets for investigation. In this study, the Forensic Microbiome Database (FMD) (http://www.fmd.jcvi.org) containing the microbiome data of various locations in the human body in 35 countries was used. We focused on skin, saliva, vaginal fluid, and stool and found that the microbiome distribution differed according to the body part as well as the geographic location. In the case of skin samples, Staphylococcus species were higher than Corynebacterium species among Asians compared with Americans. Holdemanella and Fusobacterium were specific in the saliva of Koreans and Japanese populations. Lactobacillus was found in the vaginal fluids of individuals in all countries, whereas Serratia and Enterobacter were endemic to Bolivia and Congo, respectively. This study is the first attempt to collate and describe the observed variation in microbiomes from the forensic microbiome database. As additional microbiome databases are reported by studies worldwide, the diversity of the applications may exceed and expand beyond the initial identification of the host.

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Diversity and the antimicrobial activity of vaginal lactobacilli: current status and future prospective

Purkayastha

Bhattacharya

Prasad

et al. 2020

Recent Advancements in Microbial Diversity

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Diversity of cultivable vaginal microbiota in asymptomatic women of reproductive age in Mumbai, India

Cited by 4 publications

References 0 publications

Contrasting diversity of vaginal lactobacilli among the females of Northeast India

Contrasting diversity of vaginal lactobacilli among the females of Northeast India

Forensic Analysis of Human Microbiome in Skin and Body Fluids Based on Geographic Location

Diversity and the antimicrobial activity of vaginal lactobacilli: current status and future prospective

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