Diversity of Bradyrhizobia from 27 Tropical Leguminosae Species Native of Senegal

Doignon-Bourcier, F; Sy, Abdoulaye; Willems, Anne; Torck, U; Dreyfus, B; Gillis, Monique; Lajudie, Philippe de

doi:10.1016/s0723-2020(99)80018-6

Cited by 41 publications

(27 citation statements)

References 47 publications

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“…We also performed ARDRA to compare the 20 new isolates with Bradyrhizobium strains representative of the ARDRA groups previously described (13,26). The results are shown as a dendrogram in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…strains from Aeschynomene. The two other main clusters, containing the type strains of the three named Bradyrhizobium species, were previously described by Doignon-Bourcier et al (13).…”

Section: Resultsmentioning

confidence: 99%

“…All strains were stored at Ϫ80°C on YEM medium adjusted to 20% (vol/vol) glycerol. Representative and type strains of Bradyrhizobium japonicum and Bradyrhizobium elkanii and various clusters of Bradyrhizobium strains previously described (13,15,26,27) were included in this study (Table 2).…”

Section: Methodsmentioning

confidence: 99%

“…Total DNA was purified by a slightly modified technique from Pitcher et al (31) described by Doignon-Bourcier et al (13). The primers and the general conditions for 16S rRNA gene PCR amplification were those described by Doignon-Bourcier et al (13) except that amplifications were carried out in a Gene Amp PCR System 2400 (Perkin-Elmer). Endonuclease restriction of PCR products was performed with HinfI, DdeI, MwoI, AluI, and HhaI.…”

Section: Methodsmentioning

confidence: 99%

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Photosynthetic Bradyrhizobia Are Natural Endophytes of the African Wild Rice Oryza breviligulata

Chaintreuil

Giraud

Prin

et al. 2000

Appl Environ Microbiol

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139

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We investigated the presence of endophytic rhizobia within the roots of the wetland wild rice Oryza breviligulata, which is the ancestor of the African cultivated rice Oryza glaberrima. This primitive rice species grows in the same wetland sites as Aeschynomene sensitiva, an aquatic stem-nodulated legume associated with photosynthetic strains of Bradyrhizobium. Twenty endophytic and aquatic isolates were obtained at three different sites in West Africa (Senegal and Guinea) from nodal roots of O. breviligulata and surrounding water by using A. sensitiva as a trap legume. Most endophytic and aquatic isolates were photosynthetic and belonged to the same phylogenetic Bradyrhizobium/Blastobacter subgroup as the typical photosynthetic Bradyrhizobium strains previously isolated from Aeschynomene stem nodules. Nitrogen-fixing activity, measured by acetylene reduction, was detected in rice plants inoculated with endophytic isolates. A 20% increase in the shoot growth and grain yield of O. breviligulata grown in a greenhouse was also observed upon inoculation with one endophytic strain and one Aeschynomene photosynthetic strain. The photosynthetic Bradyrhizobium sp. strain ORS278 extensively colonized the root surface, followed by intercellular, and rarely intracellular, bacterial invasion of the rice roots, which was determined with a lacZ-tagged mutant of ORS278. The discovery that photosynthetic Bradyrhizobium strains, which are usually known to induce nitrogen-fixing nodules on stems of the legume Aeschynomene, are also natural true endophytes of the primitive rice O. breviligulata could significantly enhance cultivated rice production.

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Section: Resultsmentioning

confidence: 99%

“…strains from Aeschynomene. The two other main clusters, containing the type strains of the three named Bradyrhizobium species, were previously described by Doignon-Bourcier et al (13).…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Photosynthetic Bradyrhizobia Are Natural Endophytes of the African Wild Rice Oryza breviligulata

Chaintreuil

Giraud

Prin

et al. 2000

Appl Environ Microbiol

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139

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“…In particular, the precise taxonomical status of many Bradyrhizobium sp. strains isolated from various legumes is not clarified (9,10,25,26,27,34). For this genus (Bradyrhizobium), several authors have reported the lack of consistency between results obtained by different taxonomic techniques (10,20,32,45).…”

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confidence: 99%

Genotypic Characterization of Bradyrhizobium Strains Nodulating Small Senegalese Legumes by 16S-23S rRNA Intergenic Gene Spacers and Amplified Fragment Length Polymorphism Fingerprint Analyses

Doignon-Bourcier

Willems

Coopman

et al. 2000

Appl Environ Microbiol

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We examined the genotypic diversity of 64 Bradyrhizobium strains isolated from nodules from 27 native leguminous plant species in Senegal (West Africa) belonging to the genera Abrus, Alysicarpus, Bryaspis, Chamaecrista, Cassia, Crotalaria, Desmodium, Eriosema, Indigofera, Moghania, Rhynchosia, Sesbania, Tephrosia, and Zornia, which play an ecological role and have agronomic potential in arid regions. The strains were characterized by intergenic spacer (between 16S and 23S rRNA genes) PCR and restriction fragment length polymorphism (IGS PCR-RFLP) and amplified fragment length polymorphism (AFLP) fingerprinting analyses. Fifty-three reference strains of the different Bradyrhizobium species and described groups were included for comparison. The strains were diverse and formed 27 groups by AFLP and 16 groups by IGS PCR-RFLP. The sizes of the IGS PCR products from the Bradyrhizobium strains that were studied varied from 780 to 1,038 bp and were correlated with the IGS PCR-RFLP results. The grouping of strains was consistent by the three methods AFLP, IGS PCR-RFLP, and previously reported 16S amplified ribosomal DNA restriction analysis. For investigating the whole genome, AFLP was the most discriminative technique, thus being of particular interest for future taxonomic studies in Bradyrhizobium, for which DNA is difficult to obtain in quantity and quality to perform extensive DNA:DNA hybridizations.Due to their nitrogen-fixing symbiosis with soil bacteria collectively named rhizobia, legumes play an important role in the nitrogen cycle, especially in the tropics. They are used to restore or increase soil fertility of degraded soils in intercropping systems, as green manure, and to produce medicinal and commercial by-products. In Senegal (West Africa), many native legumes are important plant resources for many purposes, and they are the only alternatives to the costly and pollutive mineral nitrogen fertilizers used in agriculture. Many of these legumes are well adapted to the local arid climatic conditions. As drought and soil erosion progress, they are still present as colonizers. The family Leguminosae is considered to be of tropical or subtropical origin, and many of the recently proposed new rhizobial species originate from leguminous plants from these zones that had not been previously investigated.After several prospections around the country, we focused on 27 native nodulated leguminous plants species belonging to the genera Abrus, Alysicarpus, Bryaspis, Chamaecrista, Cassia, Crotalaria, Desmodium, Eriosema, Indigofera, Moghania, Rhynchosia, Sesbania, Tephrosia, Zornia, which play an important ecological role and have agronomic potential in arid regions. As very little or no information concerning their associated rhizobia were available so far, we obtained 71 nodule isolates from these legumes in different regions in Senegal and first characterized them by sodium dodecyl sulfate-polyacrylamide gel electrophoresis whole-cell protein profiles and by 16S amplified ribosomal DNA restriction analysis (ARDRA) fing...

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Bradyrhizobium, the Ancestor of All Rhizobia: Phylogeny of Housekeeping and Nitrogen‐Fixation Genes

Hungría

Menna

Delamuta

2015

Biological Nitrogen Fixation

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Diversity of Bradyrhizobia from 27 Tropical Leguminosae Species Native of Senegal

Cited by 41 publications

References 47 publications

Photosynthetic Bradyrhizobia Are Natural Endophytes of the African Wild Rice Oryza breviligulata

Photosynthetic Bradyrhizobia Are Natural Endophytes of the African Wild Rice Oryza breviligulata

Genotypic Characterization of Bradyrhizobium Strains Nodulating Small Senegalese Legumes by 16S-23S rRNA Intergenic Gene Spacers and Amplified Fragment Length Polymorphism Fingerprint Analyses

Bradyrhizobium, the Ancestor of All Rhizobia: Phylogeny of Housekeeping and Nitrogen‐Fixation Genes

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