Diversity of bacteriocins produced by lactic acid bacteria isolated from raw milk

Rodrı́guez, E.; González, Beatriz; Gaya, Pilar; Núñez, Manuel; Médina, Margarita

doi:10.1016/s0958-6946(00)00017-0

Cited by 131 publications

(94 citation statements)

References 29 publications

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“…Numerous reports on bacteriocins from lactic acid bacteria (LAB) are available, and the literature has been reviewed extensively in recent years [10,12,20,26,32]. However, most papers deal with bacteriocins produced by various lactococci, pediococci, leuconostoc and enterococci, and relatively little is known about bacteriocins from Streptococcus thermophilus species.…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of thermophilin ST-1, a novel bacteriocin produced by Streptococcus thermophilus ACA-DC 0001

Aktypis

Kalantzopoulos

2003

Lait

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-Thermophilin ST-1 is produced by Streptococcus thermophilus ACA-DC 0001, a "wild" strain isolated from traditional Greek yogurt products. It exerts an inhibitory effect on lactic acid bacteria, several food spoilage and food-borne pathogenic microorganisms, and some Gramnegative phytopathogen bacteria, including Listeria innocua BL 86/20 , Enterococcus faecalis EF1, Staphylococcus aureus ATCC 29996, Xanthomonas campestris BPIC 1660, Pseudomonas syringae BPIC 1549 and Erwinia rubrifasciens BPIC 1710. The crude antimicrobial compound is heat-labile (60°C for 10 min) and sensitive to the proteolytic enzymes pronase and trypsin and high acidic and alkaline conditions, and shows a bactericidal mode of action against the indicator strain Lactococcus lactis ssp. cremoris CNRZ-117. Production of thermophilin ST-1 starts during the early growth of the producer strain and reaches a maximum titer of 2560 AU·mL -1 at the end of the exponential growth. Thermophilin ST-1 was partially purified by ammonium sulfate precipitation, ionexchange and size-exclusion chromatography. SDS-PAGE electrophoresis of purified thermophilin ST-1 showed a single protein band with a molecular mass of 30 kg·mol -1 , classifying this novel bacteriocin with the large heat-labile proteins. Until now, however, the molecular mass of bacteriocins reported in the species of S. thermophilus was less than 10 kg·mol -1 (small, heat-stable peptides). Curing experiments did not result in the loss of bacteriocin production, suggesting that the genetic determinant is probably located on the chromosome. Streptococcus thermophilus

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Section: Introductionmentioning

confidence: 99%

Purification and characterization of thermophilin ST-1, a novel bacteriocin produced by Streptococcus thermophilus ACA-DC 0001

Aktypis

Kalantzopoulos

2003

Lait

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“…Almost 70% of the isolates exhibited antimicrobial activity against one or more indicator bacteria. This corresponds to a higher proportion than often described in other investigations (9,12,21). Among lactic acid bacteria isolated from raw milk, 82 of 298 strains (27.5%) displayed bacteriocin activity (21), while 8.7% of S. aureus strains isolated from cattle produced antimicrobial substances (17).…”

Section: Discussionmentioning

confidence: 68%

Screening for antimicrobial activity among bacteria isolated from the Amazon Basin

Motta¹,

Cladera-Olivera²,

Brandelli³

2004

Braz. J. Microbiol.

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Bacteria producing antimicrobial activity were identified among 86 isolates from aquatic environments of Brazilian Amazon Basin. Antimicrobial activity against at least one indicator strain was detected for 59 isolates (68.6%). Inhibitory activity was mostly against Gram-positive bacteria, such as Listeria monocytogenes and Bacillus cereus. The antimicrobial substances produced by 19 strains that showed higher inhibitory activity were partially characterized. These antimicrobial substances showed thermal resistance for up to 100°C and partial resistance to proteolytic treatment. Some antimicrobials were partially resistant to pronase E at 2 mg ml -1 or to treatment with trichloroacetic acid. Detection of antimicrobial activity on polyacrylamide gels revealed that the molecular weight of the compounds was lower than 14 kDa. Several strains showed antibacterial activity, which in some cases appear to be related to antimicrobial peptides. The potential of these microorganisms to produce useful antimicrobial compounds is great and must be better explored.

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“…In this study in order to rule out the antagonistic effect of acidity of the culture supernatant, neutralization of CFCS was done using NaOH. All the three isolates tested in this study showed activity against L. monocytogenes, food spoilage bacteria which is relevant in the food and dairy industries [33] [34].…”

Section: Discussionmentioning

confidence: 78%

Isolation and Identification of Potential Probiotic Bacteria from Cattle Farm Soil in Dibrugarh District

Siraj¹,

Sood²,

Yadav³

2017

AiM

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Several studies have been done to isolate probiotic bacteria from different sources. In this present study, an attempt was made to isolate, screen and identify potential probiotic bacteria from cattle farm soil in Dibrugarh district, Assam, India. At the level of screening, the result showed the isolates designated as DUA4, DUD3 and DUE2 showed a percent survival rate of 75.36, 69.14 and 52.36 respectively at a pH of 2.5. Similarly survival rate of the same isolates in 0.5% bile salt condition was found to be 117.17%, 144.59% and 118.10% for the isolates DUA4, DUD3 and DUE2 respectively. Antimicrobial activity of the isolates towards the indicator organisms tested showed that DUA4 inhibited gram positive organisms while DUD3 showed activity against both gram positive and gram negative bacteria. All the three isolates showed activity against L. monocytogenes. Autoaggregation ability of the isolates DUA4, DUD3 and DUE2 was found to be 44.15%, 54.11% and 9.42% respectively. The adhesion ability of the isolates DUD3, DUA4 and DUE2 to xylene was 61.78%, 45.37% and 14.83% respectively. Antimicrobial susceptibility test of the isolates showed that the isolates are in general sensitive to antibiotics tested. Phenotypic and genotypic analysis based on the 16S rRNA gene sequence of the isolates DUA4, DUD3 and DUE2 resulted in the identification and designation of the isolate DUA4 as Bacillus spp., DUD3 as Enterococcus faecium and DUE2 as Enterobacter sp. In conclusion, the study has indicated the possibility of isolating potential probiotic bacterial strains from cattle farm soil.

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Diversity of bacteriocins produced by lactic acid bacteria isolated from raw milk

Cited by 131 publications

References 29 publications

Purification and characterization of thermophilin ST-1, a novel bacteriocin produced by Streptococcus thermophilus ACA-DC 0001

Purification and characterization of thermophilin ST-1, a novel bacteriocin produced by Streptococcus thermophilus ACA-DC 0001

Screening for antimicrobial activity among bacteria isolated from the Amazon Basin

Isolation and Identification of Potential Probiotic Bacteria from Cattle Farm Soil in Dibrugarh District

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