Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, China

Yu, Yang; Shi, Wuyang; Wan, Minxi; Zhang, Yanfei; Zou, Lihong; Huang, Jufang; Qiu, Guanzhou; Liu, Xuedan

doi:10.2225/vol11-issue1-fulltext-6

Cited by 15 publications

(9 citation statements)

References 27 publications

(43 reference statements)

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“…Our result is opposite with it, and the reason probably is that the correlation between dsr abundance and metals is metal type dependent. As for sulfur oxidation genes, it is well known that metals and S always coexist in extreme environments 35 , like sulfide minerals ore or acid mine drainage (AMD), which can explain the correlation between sulfur oxidation genes and metals.…”

Section: Discussionmentioning

confidence: 99%

An integrated insight into the response of sedimentary microbial communities to heavy metal contamination

Yin

Niu

Ren

et al. 2015

Sci Rep

210

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Response of biological communities to environmental stresses is a critical issue in ecology, but how microbial communities shift across heavy metal gradients remain unclear. To explore the microbial response to heavy metal contamination (e.g., Cr, Mn, Zn), the composition, structure and functional potential of sedimentary microbial community were investigated by sequencing of 16S rRNA gene amplicons and a functional gene microarray. Analysis of 16S rRNA sequences revealed that the composition and structure of sedimentary microbial communities changed significantly across a gradient of heavy metal contamination, and the relative abundances were higher for Firmicutes, Chloroflexi and Crenarchaeota, but lower for Proteobacteria and Actinobacteria in highly contaminated samples. Also, molecular ecological network analysis of sequencing data indicated that their possible interactions might be enhanced in highly contaminated communities. Correspondently, key functional genes involved in metal homeostasis (e.g., chrR, metC, merB), carbon metabolism, and organic remediation showed a higher abundance in highly contaminated samples, indicating that bacterial communities in contaminated areas may modulate their energy consumption and organic remediation ability. This study indicated that the sedimentary indigenous microbial community may shift the composition and structure as well as function priority and interaction network to increase their adaptability and/or resistance to environmental contamination.

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Section: Discussionmentioning

confidence: 99%

An integrated insight into the response of sedimentary microbial communities to heavy metal contamination

Yin

Niu

Ren

et al. 2015

Sci Rep

210

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“…While the neutral pH optimum for thiobacilli between 6 and 8 is consistent with the soil pH of the 6‐year‐old site, acidithiobacilli are obligate acidiphiles reported from acidic mine drainage waters, biofilms or mine sediments (Johnson et al ., 2001; Johnson & Hallberg, 2003; Macalady et al ., 2007) with pH between 0 and 4. In such highly acidic environments, A. ferrooxidans was reported to dominate bacterial communities, with abundances ranging up to 70% (Gonzalez‐Toril et al ., 2003; Mendez et al ., 2008; Yang et al ., 2008). This study is the first report on A. ferrooxidans being highly abundant in an apparently neutral pH and indicates the probable existence of pH gradients associated with acidic microniches (Robertson & Kuenen, 2006).…”

Section: Discussionmentioning

confidence: 99%

Development of bacterial community during spontaneous succession on spoil heaps after brown coal mining

Urbanová

Kopecký

Valášková

et al. 2011

FEMS Microbiology Ecology

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Changes in the abundance of bacteria and fungi and in the composition of bacterial communities during primary succession were investigated in a brown coal mine deposit area near Sokolov, the Czech Republic, using phospholipid fatty acids analysis, microarray and 16S rRNA gene sequencing. The study considered a chronosequence of sites undergoing spontaneous succession: 6-, 12-, 21- and 45-year-old and a 21-year-old site revegetated with Alnus glutinosa. During succession, organic carbon and the total nitrogen content increased while the pH and the C/N ratio decreased. Microbial biomass and bacterial diversity increased until 21 years and decreased later; bacteria dominated over fungi in the initial and late phases of succession. Bacterial community composition of the 6-year-old site with no vegetation cover largely differed from the older sites, especially by a higher content of Gammaproteobacteria, Cyanobacteria and some Alphaproteobacteria. Bacteria belonging to the genera Acidithiobacillus, Thiobacillus and related taxa, the CO(2) and N(2) fixers, dominated the community at this site. In the later phases, bacterial community development seemed to reflect more the changes in soil nutrient content and pH than vegetation with a decrease of Actinobacteria and an increase of Acidobacteria. The site revegetated with A. glutinosa resembled the 45-year-old primary succession site and exhibited an even lower pH and C/N ratio, indicating that recultivation is able to accelerate soil development.

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“…The low microbial diversity according to SSCP analysis was consistent with the results of previous work in a similar environment. Yang et al (2008) have used ARDRA (amplified ribosomal DNA restriction analysis) analysis to characterize the diversity and community structure of Acide Mine drainage. They showed that pH and polluant concentrations exert a considerable influence on the phylogenetic diversity of microbial communities in mining sites.…”

Section: Resultsmentioning

confidence: 99%

“…and Ralstonia pickettii. These bacteria are known for their distinctive ability to live in low-nutrient environments, a characteristic of most heavily metalcontaminated sites (Yang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Analysis of phosphoric ore bacterial and eucaryal microbial diversity by single strand conformation polymorphism (SSCP) and small-subunit (SSU) sequencing

Omri¹,

Manaii²,

Tlili³

et al. 2014

Afr. J. Biotechnol.

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The aim of this study was to investigate the phosphoric ore bacterial and eucaryal microbial diversity by using the single strand conformation polymorphism (SSCP) technique and small-subunit (SSU) sequencing. PCR-SSCP patterns showed a remarkably simple microbial community, mainly for bacterial community, but also for the eukaryotic community. According to the highly bacterial variable V3 region of 16S rRNA sequences, five bacterial species were identified: an unidentified species belonged to proteo gamma phylum, an unidentified species belonging to proteo delta phylum, one belonged to Moraxellaceae and two closed to Pseudomonadaceae as predominant bacteria in the mining residue. The study showed for the first time that phosphoric ore harbors major bacterial groups related to organisms having a wide range of environmentally significant functional attributes. These findings provided new opportunities into phosphoric ore microbiology that could be useful in biological system removing waste gases generated from the phosphoric industry.

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Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, China

Cited by 15 publications

References 27 publications

An integrated insight into the response of sedimentary microbial communities to heavy metal contamination

An integrated insight into the response of sedimentary microbial communities to heavy metal contamination

Development of bacterial community during spontaneous succession on spoil heaps after brown coal mining

Analysis of phosphoric ore bacterial and eucaryal microbial diversity by single strand conformation polymorphism (SSCP) and small-subunit (SSU) sequencing

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