Diversity of auxin‐producing bacteria associated to Pseudomonas savastanoi ‐induced olive knots

Abstract

Forty three strains were isolated from knots induced by Pseudomonas savastanoi in different olive cultivars. All the selected bacteria were shown to produce variable amounts of the plant growth hormone indole-3-acetic acid (IAA). Amplification of the intergenic transcribed spacers (ITS) between 16S and 23S rDNA genes, allowed the clustering of the isolates into seven distinct groups. All isolates from ITS group 1 were positive to the Pseudomonas savastanoi pv. savastanoi specific iaa L gene as shown by PCR. Partial sequencing of 16S rDNA gene confirmed the identity of these isolates to Pseudomonas savastanoi strains and allowed to tentatively assign the other isolates from the remaining ITS groups to Pantoea oleae/agglomerans, Burkholderia cepacia, Pseudomonas putida, Stenotrophomonas maltophilia and Hafnia alvei. Identification of endophytic knot-derived isolates revealed association of various saprophytic and putative human pathogenic bacteria with P. savastanoi pv. savastanoi in knot environment of olive infected trees.

Diversity and Antimicrobial Properties of Lactic Acid Bacteria Isolated from Rhizosphere of Olive Trees and Desert Truffles of Tunisia

“…Different other microbial species were used in antimicrobial testing. Pseudomonas savastanoi knW2, Pantoea agglomerans kn45, and Stenotrophomonas maltophilia KnT2 were previously isolated from olive knots [22]. Listeria monocytogenes L15 and Botrytis cinerea were obtained from the Laboratory of Microorganisms and Active Biomolecules (LMBA), Faculty of Sciences of Tunis.…”

Influence of Biological Treatment and Ultraviolet Disinfection System on Pseudomonas spp. Diversity in Wastewater as Assessed by Denaturing Gradient Gel Electrophoresis

“…16S rDNA genes from nine strains used to compare DGGE profiles are also amplified with the same primers (PsF, PsR, and F968‐GC, Psr). * PsWw.128: P. fluorescens (I), PsS.83: P. putida (I), PsS.73: P. fluorescens (II), PsS.79: P. putida (II), PsWt.146: P. putida (II), PsTp.171: P. mosselii , PsWw.118: P. otitidis , PsC.132: P. aeruginosa and Syr: P. syringae . Access numbers of selected strains are respectively: HM627582‐HM627581‐HM627594‐HM627629‐HM627604‐HM627603‐HM627606‐HM627574‐AM265392.…”

“…16S rDNA genes from nine strains used to compare DGGE profiles are also amplified with the same primers (PsF, PsR, and F968-GC, Psr). [24] and Syr: P. syringae [25]. Access numbers of selected strains are respectively: HM627582-HM627581-HM627594-HM627629-HM627604-HM627603-HM627606-HM627574-AM265392.…”

Isolation and characterization of non-Frankia actinobacteria from root nodules of Alnus glutinosa, Casuarina glauca and Elaeagnus angustifolia

“…One hundred microlitres of the resulting cell-free supernatants (CFS) were placed in the prepared wells. The inhibition zone diameters were measured, after sufficient growth of the indicator strain, as described in Ouzari et al (2008).…”