Diversity of arbuscular mycorrhizal fungi in asphalt post-mining land in Buton Island, Indonesia

Abstract

Abstract. Tuheteru FD, Husna, Albasri, Effendy HM, Arif A, Basrudin, Tuheteru EJ, Mulyono S, Irianto RSB. 2022. Diversity of arbuscular mycorrhizal fungi in asphalt post-mining land in Buton Island, Indonesia. Biodiversitas 23: 6327-6334. Studies on the presence and diversity of Arbuscular Mycorrhizal Fungi (AMF) on overburden (OB) and asphalt post-mining areas in Buton Island are still limited. The purpose of this study was to examine the presence and diversity of AMF on OB and asphalt post-mining stockpiles in Buton Island, Southeast Sulawesi Province. The sampling of soil and roots of successional plants was carried out at eight post-mining sites at the IUP Kabungka (Pasarwajo) (6 sites) PT. WIKA Aspal and IUP Lawele (2 sites), PT. WIKA Bitumen. The AMF spores were isolated using the wet filter pour method, followed by the identification of AMF which was done by observing the morphology of AMF spores. The results showed that all successional plants were in symbiosis with AMF, which was indicated by the existence of AMF structure in plant roots with the percentage of AMF colonization ranging from 5 - 95%. The number of spores per 50 g ranged from 1 to 9 spores. A total of 9 AMF species were found to belong to five genera, i.e., Claroideoglomus, Haloatospora, Glomus, Gigaspora, and Cetraspora from three families. Claroideoglomus claroideum was found to be the most dominant species isolated with the high occurrence and the highest Importance and Shannon-Wiener Diversity Indices followed by Haloatospora cf. pansihalos, Glomus sp. 5, and Glomus sp. 4. This study also showed that the AMF diversity index differed among locations. The presence of AMF can accelerate the emergence and development of adaptive plants on post-asphalt mining land in Buton Island.

Soil properties change, and arbuscular mycorrhizal fungi associated with plants growing on the post-gold mining land of Bombana, Indonesia

“…Results of this study showed that the dominant AMF family was Glomeraceae at a percentage of 70%. Several studies also showed the same results, in which Glomeraceae was dominant in the post-mining area of nickel (Prayudyaningsih et al, 2019), coal (Ezeokoli et al, 2020), asphalt (Tuheteru et al, 2022), gold (Tuheteru et al, 2020), and limestone (Suting and Devi, 2021). Glomeracecae was reported to be dominant in the rhizosphere of several tropical tree species .…”

“…AMF served as a tool for restoring the degraded land and the disturbed ecosystem (Asmelash et al, 2016), and for the post-mining land (de Moura et al, 2022) in particular. Several studies showed that AMF was capable of accelerating the succession of natural vegetation in several post-mining land conditions worldwide, including coal (Husin et al, 2017), gold (Tuheteru et al, 2020), and asphalt (Tuheteru et al, 2022). According to previous reports, AMF could improve the growth of tropical tree species in soil media from a post-gold mining land at a nursery and field scale in Bombana (Arif et al, 2021;Husna et al, 2021a).…”

Reliability of the Impregnated Boron Compounds, Citric Acid- and Heat-Treated Samama (Anthocephalus macrophyllus) Wood against the Fungal and Termite Attacks

“…Havil) (Cahyono et al, 2015). Samama wood belongs to strong class II-III and durability class IV (Halawane, 2015;Tuheteru et al, 2019). This means that Samama wood has low durability and needs protection or preservation against biodeterioration agents.…”