Diversity in photosynthetic electron transport under [CO2]-limitation: the cyanobacterium Synechococcus sp. PCC 7002 and green alga Chlamydomonas reinhardtii drive an O2-dependent alternative electron flow and non-photochemical quenching of chlorophyll fluorescence during CO2-limited photosynthesis

Shimakawa, Ginga; Akimoto, Seiji; Ueno, Yoshifumi; Wada, Ayumi; Shaku, Keiichiro; Takahashi, Yuichiro; Miyake, Chikahiro

doi:10.1007/s11120-016-0253-y

Cited by 20 publications

(39 citation statements)

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“…In this study, a simultaneous measurement of oxygen concentration and Chl fluorescence was performed in S. 7002 Dflv1/3 grown under ambient [CO 2 ] (Supplemental Fig. S4), indicating that FLV1/3 is the molecular mechanism of the oxygen-dependent AEF we found in S. 7002 (Shimakawa et al, 2016).…”

Section: Effects Of Flv On the Photosynthetic Parameters Of Psii And mentioning

confidence: 76%

“…3; Supplemental Fig. S4; Shimakawa et al, 2016). In S. 7002, the lack of FLV-mediated AEF resulted in P700 reduction, photosynthesis suppression, PSI photoinhibition, and growth retardation (Figs.…”

Section: Effects Of Flv On the Photosynthetic Parameters Of Psii And mentioning

confidence: 95%

“…Recently, we found that S. 7002 drives an oxygen-dependent AEF to restore linear electron transport during CO 2 -limited photosynthesis. This process is particularly evident in cells grown under ambient [CO 2 ] (Shimakawa et al, 2016). In this study, a simultaneous measurement of oxygen concentration and Chl fluorescence was performed in S. 7002 Dflv1/3 grown under ambient [CO 2 ] (Supplemental Fig.…”

Section: Effects Of Flv On the Photosynthetic Parameters Of Psii And mentioning

confidence: 99%

“…Finally, plastoquinol accumulation inhibits the Q-cycle turnover in the Cyt b 6 /f complex, which suppresses electron flow from the Cyt b 6 /f complex to P700. This reaction is called the reduction-induced suppression of electron flow (RISE; Shaku et al, 2016). Overall, these molecular mechanisms contribute to P700 oxidation, thereby preventing PSI photoinhibition and enabling oxygenic phototrophs to thrive.…”

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Oxidation of P700 in Photosystem I Is Essential for the Growth of Cyanobacteria

2016

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The photoinhibition of photosystem I (PSI) is lethal to oxygenic phototrophs. Nevertheless, it is unclear how photodamage occurs or how oxygenic phototrophs prevent it. Here, we provide evidence that keeping P700 (the reaction center chlorophyll in PSI) oxidized protects PSI. Previous studies have suggested that PSI photoinhibition does not occur in the two model cyanobacteria, Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942, when photosynthetic CO 2 fixation was suppressed under low CO 2 partial pressure even in mutants deficient in flavodiiron protein (FLV), which mediates alternative electron flow. The lack of FLV in Synechococcus sp. PCC 7002 (S. 7002), however, is linked directly to reduced growth and PSI photodamage under CO 2 -limiting conditions. Unlike Synechocystis sp. PCC 6803 and S. elongatus PCC 7942, S. 7002 reduced P700 during CO 2 -limited illumination in the absence of FLV, resulting in decreases in both PSI and photosynthetic activities. Even at normal air CO 2 concentration, the growth of S. 7002 mutant was retarded relative to that of the wild type. Therefore, P700 oxidation is essential for protecting PSI against photoinhibition. Here, we present various strategies to alleviate PSI photoinhibition in cyanobacteria.Low CO 2 fixation efficiency in the Calvin-Benson cycle prevents the utilization of NADPH and ATP in photosynthesis and causes these molecules to accumulate, resulting in oxidative photosynthetic cell damage. High light, low temperature, and CO 2 limitation increase NADPH and ATP levels beyond the Calvin-Benson cycle requirements. Electrons and H +

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Section: Effects Of Flv On the Photosynthetic Parameters Of Psii And mentioning

confidence: 76%

Section: Effects Of Flv On the Photosynthetic Parameters Of Psii And mentioning

confidence: 95%

Section: Effects Of Flv On the Photosynthetic Parameters Of Psii And mentioning

confidence: 99%

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confidence: 99%

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Oxidation of P700 in Photosystem I Is Essential for the Growth of Cyanobacteria

2016

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“…PCC 7002), under suppressed photosynthesis conditions equilibrated with air, AEF is driven by FLVs, not by photorespiration (Hayashi et al, 2014;Shimakawa et al, 2015Shimakawa et al, , 2016bShaku et al, 2016). Furthermore, some eukaryotic algae, including the green alga Chlamydomonas reinhardtii and the diatom Phaeodactylum tricornutum, do not utilize photorespiration as the main alternative electron sink under suppressed photosynthesis conditions (Shimakawa et al, 2016a(Shimakawa et al, , 2017. These facts suggest that oxygenic phototrophs that reside in or are exposed to aqueous environments use FLV in AEF to oxidize P700 in PSI.…”

Section: Resultsmentioning

confidence: 99%

The Liverwort, Marchantia, Drives Alternative Electron Flow Using a Flavodiiron Protein to Protect PSI

et al. 2017

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The diffusion efficiency of oxygen in the atmosphere, like that of CO 2 , is approximately 10 4 times greater than that in aqueous environments. Consequently, terrestrial photosynthetic organisms need mechanisms to protect against potential oxidative damage. The liverwort Marchantia polymorpha, a basal land plant, has habitats where it is exposed to both water and the atmosphere. Furthermore, like cyanobacteria, M. polymorpha has genes encoding flavodiiron proteins (FLV). In cyanobacteria, FLVs mediate oxygen-dependent alternative electron flow (AEF) to suppress the production of reactive oxygen species. Here, we investigated whether FLVs are required for the protection of photosynthesis in M. polymorpha. A mutant deficient in the FLV1 isozyme (DMpFlv1) sustained photooxidative damage to photosystem I (PSI) following repetitive short-saturation pulses of light. Compared with the wild type (Takaragaike-1), DMpFlv1 showed the same photosynthetic oxygen evolution rate but a lower electron transport rate during the induction phase of photosynthesis. Additionally, the reaction center chlorophyll in PSI, P700, was highly reduced in DMpFlv1 but not in Takaragaike-1. These results indicate that the gene product of MpFlv1 drives AEF to oxidize PSI, as in cyanobacteria. Furthermore, FLV-mediated AEF supports the production of a proton motive force to possibly induce the nonphotochemical quenching of chlorophyll fluorescence and suppress electron transport in the cytochrome b 6 /f complex. After submerging the thalli, a decrease in photosystem II operating efficiency was observed, particularly in DMpFlv1, which implies that species living in these sorts of habitats require FLV-mediated AEF.

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Interaction of pretilachlor with PS‐II activity of the cyanobacterium Desmonostoc muscorum PUPCCC 405.10

et al. 2020

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Interaction of pretilachlor with photosystem (PS)‐II of the cyanobacterium Desmonostoc muscorum PUPCCC 405.10 has been studied in this paper. Pretilachlor negatively affected growth, chlorophyll a (Chl a), photosynthesis, and carbon dissimilation in a dose‐dependent manner. Effects were also observed in PSs, especially PS‐II (an 11–35% decrease), as well as the whole photosynthetic electron transport activity. The fluorescence emission spectrum of Chl a revealed a dose‐dependent effect of pretilachlor on both the antenna and the core complex of PSs, with more severe effect on the former. Data of O‐J‐I‐P fluorescence transient of Chl a revealed that pretilachlor interfered with electron flow between QA and QB sites of PS‐II. It was further observed that pretilachlor decreased maximum fluorescence, variable and relative variable fluorescence, maximum quantum yield, quantum yield of electron transport, the rate of trapped exciton movement, quantum yield of electron transfer, and performance index of primary photochemistry; however, there was a progressive increase in the net rate of PS‐II closure, quantum yield of energy dissipation, and effective antenna size per active reaction center. A decrease in photosynthetic activity leads to a decrease in carbon dissimilation, as evidenced by low activity of glucose‐6‐phosphate dehydrogenase and pyruvate kinase. Thus, pretilachlor, which is otherwise known to kill weeds by interfering with cell division, affected the growth of the cyanobacteria by interacting with PS‐II.

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Diversity in photosynthetic electron transport under [CO2]-limitation: the cyanobacterium Synechococcus sp. PCC 7002 and green alga Chlamydomonas reinhardtii drive an O2-dependent alternative electron flow and non-photochemical quenching of chlorophyll fluorescence during CO2-limited photosynthesis

Cited by 20 publications

References 63 publications

Oxidation of P700 in Photosystem I Is Essential for the Growth of Cyanobacteria

Oxidation of P700 in Photosystem I Is Essential for the Growth of Cyanobacteria

The Liverwort, Marchantia, Drives Alternative Electron Flow Using a Flavodiiron Protein to Protect PSI

Interaction of pretilachlor with PS‐II activity of the cyanobacterium Desmonostoc muscorum PUPCCC 405.10

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