Diversity, function and assembly of the Trifolium repens L. root-associated microbiome under lead stress

Gong, Li; Gan, Deping; Li, Xinying; Yao, Jiaxuan; Wang, Lei; Qu, Jianhua; Cong, Jingmin; Zhang, Ying

doi:10.1016/j.jhazmat.2022.129510

Cited by 19 publications

(5 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As a Pb-resistant strain, Proteobacteria was tolerant to Pb(II). Therefore, when algal-bacterial granular sludge was exposed to Pb(II) for a long time, the relative abundance of Proteobacteria increased [34]. The algae in the algal-bacterial granular sludge used in this study were cyanobacteria.…”

Section: Changes In Microbial Communitiesmentioning

confidence: 78%

Over-Produced Extracellular Polymeric Substances and Activated Antioxidant Enzymes Attribute to Resistance of Pb(II) for Algal–Bacterial Granular Sludge in Municipal Wastewater Treatment

Yang,

Zhang,

Wang

2023

Water

View full text Add to dashboard Cite

Algal–bacterial granular sludge technology is a new type of wastewater treatment and resource regeneration process, which has received widespread attention due to its excellent nitrogen and phosphorus removal performance, and energy-saving and emission reduction effects. Although algal–bacterial granular sludge technology has achieved an ideal nutrient removal ability, some pol-lutants in wastewater might affect the symbiotic relationship between algae and bacteria. This study investigated the impact of coexisting Pb(II) on the symbiosis of algal–bacterial granular sludge. It was found that 2.5–10.0 mg/L of Pb(II) exposure increased the relative abundance of Pro-teobacteria. In addition, more protein in extracellular polymeric substances (EPS-PN) was secreted at 2.5 mg/L of Pb(II) exposure while EPS-PN content reduced at a rate of 5.0–10.0 mg/L of Pb(II). Under different concentrations of Pb(II), the damage degree of algal–bacterial granular sludge was exacerbated, evidenced by increased malondialdehyde (MDA) content. To cope with these adverse circumstances, the antioxidant enzyme activity of both super-oxide dismutase (SOD) and peroxidase dismutase (CAT) was boosted. With the help of these adaptive strategies, the symbiosis of algal–bacterial granular sludge was stable. Moreover, the performance of algal–bacterial granular sludge in treating COD, ammonia-N and phosphate-P was kept at above 95%. This study approved that a Pb(II) concentration less than 10.0 mg/L had little effect on the performance of algal–bacterial granular sludge in wastewater treatment. It is hoped that this study can provide useful information for an improved engineering feasibility of algal–bacterial granular sludge process.

show abstract

Section: Changes In Microbial Communitiesmentioning

confidence: 78%

Over-Produced Extracellular Polymeric Substances and Activated Antioxidant Enzymes Attribute to Resistance of Pb(II) for Algal–Bacterial Granular Sludge in Municipal Wastewater Treatment

Yang,

Zhang,

Wang

2023

Water

View full text Add to dashboard Cite

show abstract

“…These core generalist species were persistent through plant developmental stages. Furthermore, at the genus-level annotation, majority of the identified core microbes (e.g., Sphingomonas , Aeromicrobium , Pseudarthrobacter, and Rhodanobacter ) were present in various plant hosts, such as rice [ 19 ], Trifolium repens L. [ 56 ], sugarcane [ 57 ], sweetpotato [ 58 ], and Arabidopsis thaliana [ 41 ]. This indicates that these core microbes may be widespread in different plant species.…”

Section: Discussionmentioning

confidence: 99%

Nodulation number tempers the relative importance of stochastic processes in the assembly of soybean root-associated communities

Wang,

Jiao,

et al. 2023

ISME Communications

Self Cite

View full text Add to dashboard Cite

Identifying the ecological forces that structure root-associated microbial communities is an essential step toward more sustainable agriculture. Legumes are widely utilized as model plants to study selective forces and their functioning in plant-microbial interactions owing to their ability to establish mutualism with rhizobia. Root nodules act as symbiotic organs to optimize the cost-benefit balance in this mutualistic relationship by modulating the number of nodules. However, it is not known whether the number of nodules is related to the structure of root-associated bacterial communities. Here, the root-associated bacterial communities of soybean grown in native soil by means of soybean cultivars with super- or normal nodulation were investigated across four developmental stages. We compared ecological processes between communities and found decreased relative importance of neutral processes for super-nodulating soybean, although the overall structures resembled those of normal-nodulating soybean. We identified the generalist core bacterial populations in each root-associated compartment, that are shared across root-associated niches, and persist through developmental stages. Within core bacterial species, the relative abundances of bacterial species in the rhizosphere microbiome were linked to host-plant functional traits and can be used to predict these traits from microbes using machine learning algorithms. These findings broaden the comprehensive understanding of the ecological forces and associations of microbiotas in various root-associated compartments and provide novel insights to integrate beneficial plant microbiomes into agricultural production to enhance plant performance.

show abstract

“…Most of the statistical analyses were carried out by R software (V4.3.2), 17 including microbial co-occurrence network analysis, the normalized stochasticity ratio analysis (NST), the neutral community model analysis (NCM), the random forest analysis (RF), correlation analysis, the canonical correlation analysis (CCA), the redundancy analysis (RDA) and the Mantel’s test ( Wang et al, 2022 ). Partial statistical analyses were analyzed on the online platform of Majorbio Cloud Platform (see text footnote 6), including the α- diversity index (Chao index and Shannon index), the principal coordinate analysis (PCoA), microbial diversity composition, linear discriminant analysis effect size (LEfSe), linear discriminant analysis (LDA) and Procrustes analysis.…”

Section: Methodsmentioning

confidence: 99%

Enriched rhizospheric functional microbiome may enhance adaptability of Artemisia lavandulaefolia and Betula luminifera in antimony mining areas

Xing,

Gai,

Xue

et al. 2024

Front. Microbiol.

View full text Add to dashboard Cite

Dominant native plants are crucial for vegetation reconstruction and ecological restoration of mining areas, though their adaptation mechanisms in stressful environments are unclear. This study focuses on the interactions between dominant indigenous species in antimony (Sb) mining area, Artemisia lavandulaefolia and Betula luminifera, and the microbes in their rhizosphere. The rhizosphere microbial diversity and potential functions of both plants were analyzed through the utilization of 16S, ITS sequencing, and metabarcoding analysis. The results revealed that soil environmental factors, rather than plant species, had a more significant impact on the composition of the rhizosphere microbial community. Soil pH and moisture significantly affected microbial biomarkers and keystone species. Actinobacteria, Proteobacteria and Acidobacteriota, exhibited high resistance to Sb and As, and played a crucial role in the cycling of carbon, nitrogen (N), phosphorus (P), and sulfur (S). The genes participating in N, P, and S cycling exhibited metabolic coupling with those genes associated with Sb and As resistance, which might have enhanced the rhizosphere microbes’ capacity to endure environmental stressors. The enrichment of these rhizosphere functional microbes is the combined result of dispersal limitations and deterministic assembly processes. Notably, the genes related to quorum sensing, the type III secretion system, and chemotaxis systems were significantly enriched in the rhizosphere of plants, especially in B. luminifera, in the mining area. The phylogenetic tree derived from the evolutionary relationships among rhizosphere microbial and chloroplast whole-genome resequencing results, infers both species especially B. luminifera, may have undergone co-evolution with rhizosphere microorganisms in mining areas. These findings offer valuable insights into the dominant native rhizosphere microorganisms that facilitate plant adaptation to environmental stress in mining areas, thereby shedding light on potential strategies for ecological restoration in such environments.

show abstract

Diversity, function and assembly of the Trifolium repens L. root-associated microbiome under lead stress

Cited by 19 publications

References 58 publications

Over-Produced Extracellular Polymeric Substances and Activated Antioxidant Enzymes Attribute to Resistance of Pb(II) for Algal–Bacterial Granular Sludge in Municipal Wastewater Treatment

Over-Produced Extracellular Polymeric Substances and Activated Antioxidant Enzymes Attribute to Resistance of Pb(II) for Algal–Bacterial Granular Sludge in Municipal Wastewater Treatment

Nodulation number tempers the relative importance of stochastic processes in the assembly of soybean root-associated communities

Enriched rhizospheric functional microbiome may enhance adaptability of Artemisia lavandulaefolia and Betula luminifera in antimony mining areas

Contact Info

Product

Resources

About