Diversity effect of capsaicin on different types of skeletal muscle

Abstract

Capsaicin is a major pungent content in green and red peppers which are widely used as spice, and capsaicin may activate different receptors. To determine whether capsaicin has different effects on different types of skeletal muscle, we applied different concentrations (0, 0.01, and 0.02%) of capsaicin in the normal diet and conducted a four-week experiment on Sprague-Dawley rats. The fiber type composition, glucose metabolism enzyme activity, and different signaling molecules' expressions of receptors were detected. Our results suggested that capsaicin reduced the body fat deposition, while promoting the slow muscle-related gene expression and increasing the enzyme activity in the gastrocnemius and soleus muscles. However, fatty acid metabolism was significantly increased only in the soleus muscle. The study of intracellular signaling suggested that the transient receptor potential vanilloid 1 (TRPV1) and cannabinoid receptors in the soleus muscle were more sensitive to capsaicin. In conclusion, the distribution of TRPV1 and cannabinoid receptors differs in different types of muscle, and the different roles of capsaicin in different types of muscle may be related to the different degrees of activation of receptors.

TRPV1 Activation by Capsaicin Mediates Glucose Oxidation and ATP Production Independent of Insulin Signalling in Mouse Skeletal Muscle Cells

“…We evaluated the effect of capsaicin on GSK3β phosphorylation in mouse myotubes and observed that capsaicin does not significantly affect GSK3β phosphorylation. This is in agreeance with previous work which reported that GSK3β phosphorylation was not affected by capsaicin administration in rat skeletal muscles [44].…”

“…Following glucose uptake in skeletal muscle cells, glucose molecules can be added to the glycogen chain for storage through glycogenesis or produce ATP during glycolysis [43]. Glycogen synthase kinase-3 β (GSK-3β) is a serine/threonine kinase that is involved in several biological processes including glucose and glycogen metabolism and insulin action in skeletal muscle cells [44]. Insulin inhibits the activation of GSK-3β through the PI3K/AKT pathway which subsequently promotes glycogen synthase (GS) activity and glycogen synthesis in skeletal muscle cells.…”

Succinate induces skeletal muscle fiber remodeling via SUCNR1 signaling

“…Succinate dehydrogenase staining was performed as previously described . Briefly, muscle sections (10 μM) were incubated in liquid (6 mM CaCl 2 , 0.3% glacial acetic acid, pH 4.4) for 10 min, flushed by Tris‐CaCl 2 eluent buffer (0.1 M Tris, 18 mM CaCl 2 ) twice, 1 min per flush, and then incubated in 37°C pre‐heated SDH eluent buffer (0.1 M sodium succinate, 0.18 mM tetranitroblue tetrazolium chloride (NBT), 0.81% N,N‐dimethylformamide, 0.23 M Tris, pH 7.4) for 45 min.…”

Zoledronic Acid Modulation of TRPV1 Channel Currents in Osteoblast Cell Line and Native Rat and Mouse Bone Marrow-Derived Osteoblasts: Cell Proliferation and Mineralization Effect

“…This mechanism may contribute to the anti-osteoporotic effect of zoledronic acid, as well as the pain-relieving effect. Beneficial effects of zoledronic acid can be expected in skeletal muscle where TRPV1 channel appear to play a role in activating hypertrophic signaling and cytoprotection [51,52].…”