Diversity, distribution, and nest characteristics of stingless bees (Hymenoptera: Meliponini) in Baluran National Park, East Java, Indonesia

Rachmawati, Rini; Agus, Ali; Umami, Nafiatul; Agussalim, Agussalim; Purwanto, Hari

doi:10.13057/biodiv/d230805

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…This species frequently nested in human constructs in rural and suburban areas [23], in walls, trees, and pole cavities in South Gujarat, India [24]. Accordingly, the distribution of T. laeviceps in Baluran National Park, East Java, Indonesia, revealed that the bees were distributed in all sampling sites with different habitat types: teak forest and secondary forest, savanna, mangrove habitat, and rural area [25].…”

Section: Discussionmentioning

confidence: 99%

Bees and wasps in the tropical lowland rainforest transformation in Jambi, Sumatra

Soegiarso,

Raffiudin,

Sayusti

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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Bees and wasps are beneficial insects for the stability of ecosystem function. This study aimed to explore the bees and wasps and their relationship with the environmental factors in the three habitats, i.e., oil palm and rubber plantation, jungle rubber and adjacent villages in Harapan Forest, Jambi, Sumatra, Indonesia. Bees and wasps were collected using sweeping method and the environmental factors were recorded during collection time. The correlation between the number of species and environmental factors was analyzed using Principal Component Analysis (PCA). Apis and Tetragonula were the two most common bees in all habitats and villages. The number of bee and wasp species in oil palm and rubber plantations was positively correlated with temperature and light intensity but negatively correlated with humidity. We found a higher number of bee species, i.e., 26 species, compared to seven species of wasps. The highest number of bees and wasps (16 species) was found in the oil palm plantation. Both jungle rubber and adjacent villages showed the lowest number of nine species of bees and wasps. Our study implied the importance of bees and wasps representing the herbivores and predators in these transformation habitats.

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Section: Discussionmentioning

confidence: 99%

Bees and wasps in the tropical lowland rainforest transformation in Jambi, Sumatra

Soegiarso,

Raffiudin,

Sayusti

et al. 2024

IOP Conf. Ser.: Earth Environ. Sci.

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“…Thummajitsakul et al (2013) mempublikasikan hasil urutan beberapa gen mikokondria pada jenis Tetragonula pagdeni, termasuk di dalamnya gen ATPase, COI, COII, COIII, Cytb, dan 16S rRNA. Penelitian keanekaragaman lebah tanpa sengat dengan menggunakan gen 16S rRNA juga telah dilaksanakan di Indonesia, yaitu pada Taman Nasional Halimun Salak, Jawa Barat (Pratama et al, 2023), Taman Nasional Baluran, Jawa Timur (Rachmawati et al, 2022), Kabupaten Ciamis, Jawa Barat (Purwanto dan Trianto, 2021), Yogyakarta (Trianto dan Purwanto, 2020a), dan Kota Sawahlunto, Sumatera Barat (Herwina et al, 2022).…”

Section: Amplifikasi Dnaunclassified

Qualitative Analysis of Partial 16S rRNA Amplicon of Mitochondrial Gene of Stingless Bees in Pesawaran

Priyambodo,

Rustiati,

Pratiwi

et al. 2023

JBT

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Pesawaran is one of the areas that has the potential to cultivate stingless bees as producers of honey and propolis. This study was aimed at qualitatively analysing partial amplicons of the 16S rRNA gene from individuals found at the research site. The research was performed from April to September 2023 in an explorative approach. Based on the exploration that has been done in Harapan Jaya Village, Way Ratai District, Pesawaran Regency, individual stingless bees were obtained from 5 different colonies. Individual samples of stingless bees were further extracted and amplified by commercial kits. Each individual from 5 different colonies has successfully obtained its DNA amplicons. Based on qualitative analysis using 1% agarose gel, the size of the partial gene ranged from 400bp to 500bp. This result is in accordance with the data contained in the genbank, where the 16S rRNA gene size is more than 500bp. Therefore, it has been concluded that the size of the partial amplicons of the 16S rRNA gene of individual stingless bees from the 5 colonies that were obtained is actually in range of the size of the 16S rRNA gene of stingless bees that have been recorded in the genbank.

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“…PCR profiles for A. mellifera 16SrRNA are as follows: predenaturation at 95°C for 2 min, 35 cycles (denaturation at 95°C for 30 s, annealing at 52°C for 30 s, extension at 72°C for 30 s), final extension at 72°C for 5 min and final hold at 4°C (Thummajitsakul et al 2013; Nuraini and Purwanto 2021;Rachmawati et al 2022). PCR conditions for N. apis and N. ceranae 16S rRNA are as follows: predenaturation at 94°C for 5 min, 35 cycles (denaturation at 94°C for 30 s, annealing at 58°C (N. ceranae) or 66°C (N. apis) for 30 s, extension at 72°C for 1 min), and final extension at 72°C for 10 min (Ansari et al 2017;Salkova et al 2018).…”

Section: Acknowledgementsmentioning

confidence: 99%

Molecular detection of the pathogen of Apis mellifera (Hymenoptera: Apidae) in honey in Indonesia

Aditya

Purwanto

2023

Biodiversitas

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Abstract. Aditya IGMRA, Purwanto H. 2023. Molecular detection of the pathogen of Apis mellifera (Hymenoptera: Apidae) in honey in Indonesia. Biodiversitas 24: 2612-2622. Apis honey bees play a major role in global crop pollination. The honey bee population faces various threats, one being entomopathogens, responsible for 13% of honey bees' declining population cases. Some major pathogens are Nosema sp., Melissococcus plutonius, and Paenibacillus larvae. Data on the prevalence of these pathogens is not available in Indonesia. Therefore, this study aimed to detect entomopathogen in Java, Bali, and Sumatra honey. We extracted 30 honey samples from Java, Bali, and Sumatra in this study. DNA from honey amplified by conventional Polymerase Chain Reaction (PCR) to detect Nosema apis, Nosema ceranae, M. plutonius, and P. larvae. Amplicons were sequenced to confirm the species of pathogens detected. Results showed that N. ceranae has a 20% prevalence, found in Java and Bali; M. plutonius was, for the first time, found in Indonesia and only in Java, with a 3.33% prevalence. In comparison, this study didn't find N. apis and P. larvae. Environmental factors were studied for their correlation with the incidence of pathogens. From several factors, only precipitation significantly correlates with the occurrence of N. ceranae and M. plutonius, while other factors do not. This research concludes that honey can be used as source of DNA for pathogens detection and monitoring in a certain area globally.

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Diversity, distribution, and nest characteristics of stingless bees (Hymenoptera: Meliponini) in Baluran National Park, East Java, Indonesia

Cited by 7 publications

References 21 publications

Bees and wasps in the tropical lowland rainforest transformation in Jambi, Sumatra

Bees and wasps in the tropical lowland rainforest transformation in Jambi, Sumatra

Qualitative Analysis of Partial 16S rRNA Amplicon of Mitochondrial Gene of Stingless Bees in Pesawaran

Molecular detection of the pathogen of Apis mellifera (Hymenoptera: Apidae) in honey in Indonesia

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