Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white Pieris canidia (Lepidoptera: Pieridae)

Wang, Ying; Zhu, Jianqing; Fang, Jie; Shen, Li; Ma, Shuojia; Zhao, Zimiao; Yu, Weidong; Jiang, Weibin

doi:10.3390/life10110254

Cited by 14 publications

(8 citation statements)

References 35 publications

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“…For example, previous work on the diamondback moth Plutella xylostella identified two highly abundant Enterobacter species in the lepidopteran gut that played critical functional roles in detoxification and synthesis pathways (Xia et al, 2017). This result is consistent with recent work in the Indian Cabbage white Pieris canidia which reported high abundance of cellulose‐degrading Enterobacter species in the gut (Wang et al, 2020). Furthermore, some species of Enterobacter serve a protective role against Bt toxin (Raymond et al, 2009).…”

Section: Discussionsupporting

confidence: 93%

Experimental removal of extracellular egg‐associated microbes has long‐lasting effects for larval performance

et al. 2022

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Maternally transmitted microbes are ubiquitous. In insects, maternal microbes can play a role in mediating the insect immune response. Less is known about how ecological factors, such as resource use, interact with maternal microbes to affect immunity. In the context of a recent colonization of a novel host plant by the Melissa blue butterfly Lycaeides melissa, we investigated the interaction between host plant use and vertically transmitted, extracellular egg‐associated microbes in determining the strength of the insect immune response. We reared larvae on two different host plant species: a native host Astragalus canadensis and a novel host Medicago sativa. Egg‐associated microbes were removed through a series of antimicrobial egg washes prior to hatching. Immune response was measured through three assays: standing phenoloxidase (PO), total PO and melanization. We detected strong effects of microbial removal. Egg washing resulted in larvae with an increased immune response as measured by total PO—contrary to reports from other taxa. The effect of washing was especially strong for larvae consuming the native host plant. This result may explain why consumption of the egg casing is not a universal behaviour in insects, due to negative effects on larval immunity. Read the free Plain Language Summary for this article on the Journal blog.

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Section: Discussionsupporting

confidence: 93%

Experimental removal of extracellular egg‐associated microbes has long‐lasting effects for larval performance

et al. 2022

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“…The change of the intestinal bacterial community structure from Diabrotica virgifera virgifera helped promote the rapid adaptation of insects in a managed ecosystem [ 34 ]. When Pieris canidia was treated with antibiotics for gut-associated bacteria, the host’s weight was reduced after the treatment, and the effect increased with increased concentrations of the antibiotics [ 35 ]. The gut bacterial communities resulting from NSC and SAC treatments were assessed to determine the relationships between the gut microbiota, diet, and the self-regulation ability of L. xylina .…”

Section: Discussionmentioning

confidence: 99%

Gut Bacterial Communities of Lymantria xylina and Their Associations with Host Development and Diet

Cui

Chu

et al. 2021

Microorganisms

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The gut microbiota of insects has a wide range of effects on host nutrition, physiology, and behavior. The structure of gut microbiota may also be shaped by their environment, causing them to adjust to their hosts; thus, the objective of this study was to examine variations in the morphological traits and gut microbiota of Lymantria xylina in response to natural and artificial diets using high-throughput sequencing. Regarding morphology, the head widths for larvae fed on a sterilized artificial diet were smaller than for larvae fed on a non-sterilized host-plant diet in the early instars. The gut microbiota diversity of L. xylina fed on different diets varied significantly, but did not change during different development periods. This seemed to indicate that vertical inheritance occurred in L. xylina mutualistic symbionts. Acinetobacter and Enterococcus were dominant in/on eggs. In the first instar larvae, Acinetobacter accounted for 33.52% of the sterilized artificial diet treatment, while Enterococcus (67.88%) was the predominant bacteria for the non-sterilized host-plant diet treatment. Gut microbe structures were adapted to both diets through vertical inheritance and self-regulation. This study clarified the impacts of microbial symbiosis on L. xylina and might provide new possibilities for improving the control of these bacteria.

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“…After being fed with tetracycline at three different concentrations, the larval and pupal stages were significantly prolonged, and the adult stage was significantly shortened. With an increase in antibiotic concentration, the influence on the development period of P. canidia was more significant [49].…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Treatment Reduced the Gut Microbiota Diversity, Prolonged the Larval Development Period and Lessened Adult Fecundity of Grapholita molesta (Lepidoptera: Tortricidae)

Zhang

Wang

Guo

et al. 2022

Insects

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Grapholita molesta, the oriental fruit moth, is a serious pest of fruit trees with host transfer characteristics worldwide. The gut microbiota, which plays a crucial part in insect physiology and ecology, can be influenced by many elements, such as antibiotics, temperature, diet, and species. However, the effects of antibiotics on G. molesta gut microbiota are still unclear. In this study, we selected five common antibiotic agents to test the inhibition of G. molesta gut microbiota, and found ciprofloxacin shown the best antibacterial activity. After feeding 1 μg/mL of ciprofloxacin, the relative abundance of Actinobacteria and Cyanobacteria decreased significantly, while that of Firmicutes and Bacteroidetes increased. PICRUSt2 analysis indicated that most functional prediction categories were enriched in the G. molesta gut, including amino acid transport and metabolism, translation, ribosomal structure and biogenesis, carbohydrate transport and metabolism, transcription, cell wall/membrane/envelope biogenesis, and energy production and conversion. Finally, ciprofloxacin feeding significantly affected larval growth, development, and reproduction, resulting in prolonged larval development duration, shortened adult longevity, and significantly decreased single female oviposition and egg hatchability. In addition, we isolated and purified some culturable bacteria belonging to Proteobacteria, Firmicutes, Actinobacteria, and cellulase-producing bacteria from the G. molesta midgut. In brief, our results demonstrate that antibiotics can have an impact on G. molesta gut bacterial communities, which is beneficial for host growth and development, as well as helping female adults produce more fertile eggs. These results will thus provide a theoretical reference for developing new green control technology for G. molesta.

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Diversity, Composition and Functional Inference of Gut Microbiota in Indian Cabbage white Pieris canidia (Lepidoptera: Pieridae)

Cited by 14 publications

References 35 publications

Experimental removal of extracellular egg‐associated microbes has long‐lasting effects for larval performance

Experimental removal of extracellular egg‐associated microbes has long‐lasting effects for larval performance

Gut Bacterial Communities of Lymantria xylina and Their Associations with Host Development and Diet

Antibiotic Treatment Reduced the Gut Microbiota Diversity, Prolonged the Larval Development Period and Lessened Adult Fecundity of Grapholita molesta (Lepidoptera: Tortricidae)

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