Diversity, community distribution and growth promotion activities of endophytes associated with halophyte Lycium ruthenicum Murr

Abstract

The purpose of this study was to investigate the composition, diversity, distribution, and growth promotion activity of endophytic bacteria isolated from L. ruthenicum Murr. Consequently, a total of 109 endophytic bacteria affiliated to 3 phyla, 12 orders and 36 genera were isolated using nine different selective media, from which, Actinobacteria was the dominant taxon containing seven orders at the phylum level; Micrococcales showed the highest diversity containing 12 genera at the family level. Based on PAST and SPSS analysis, species diversity and abundance were mostly isolated from nutritious soil condition (22 genera) and root tissue (27 genera). Furthermore, growth phase showed significant effect on the endophytic bacteria community (28 genera at dormancy and 17 genera at fluorescence stage). With regard to ex situ plant growth-promoting activities, Streptomyces dominated and exhibited broad ability in terms of their potential to grow on nitrogen-free media, synthesize cellulase and lipase enzymes. Characterization of potential plant-beneficial traits indicate that endophytic bacteria exhibited a number of positive activities, including potential diazotrophy (n = 66), phosphate-solubilizing (n = 6), production of lipase (n = 21) and cellulose (n = 35). Two strains, representing Bacillus sp. EGI 63071 and EGI 63106, were found to be effective in promoting the growth of Triticum aestivum (wheat: Xindong No.18) seedling under salt stress conditions.

Diversity and Antimicrobial Potential of Cultivable Endophytic Actinobacteria Associated With the Medicinal Plant Thymus roseus

“…Endophytes are microorganisms that inhabit healthy plant tissues at specific growth stages, or whole stages of their life cycle, and establish a symbiotic relationship with the host without causing any apparent disease symptoms (Petrini, 1991;Duan et al, 2013;Castro et al, 2014;Egamberdieva et al, 2017bEgamberdieva et al, , 2018Li et al, 2018;Liu et al, 2019). Endophytic actinobacteria are considered to be a vital resource of microbial biodiversity (Matsumoto and Takahashi, 2017).…”

Screening of Phosphate-Solubilizing Fungi From Air and Soil in Yunnan, China: Four Novel Species in Aspergillus, Gongronella, Penicillium, and Talaromyces

“…actinomyces, bacteria, fungi, and yeasts have been screened for phosphate solubilization. They were shown to enhance the solubilization of insoluble P compounds ( Gizaw et al, 2017 ; Nandimath et al, 2017 ; Mohamad et al, 2018 ; Wang et al, 2018 ; Chen and Liu, 2019 ; Liu et al, 2019 ). For fungi, Rhizopus stolonifer var.…”

Knock, knock-let the bacteria in: enzymatic potential of plant associated bacteria

“…The production of lipases by endophytic bacteria has been detected in several analysis, being also related to their ability in plant protection, being the presence of these enzymes in strains with broad antimicrobial activity (Mohamad et al, 2018;Liu et al, 2019). Lipases and other hydrolytic enzymes studied here are related to degradation of fungal cell wall or cell membrane to protect the plants against pathogens, but also with the degradation of extracellular virulence factors and stimulating the plant immune system (Mohamad et al, 2018).…”