Diversity and temporal variations of the Hemiptera Auchenorrhyncha fauna in the Ajaccio region (France, Corsica)

Abstract

During a one-year survey of the Auchenorrhyncha fauna in a maquis habitat of the Ajaccio region (Corsica), 37 species were listed including 3 alien species recorded in Europe in the last decades. The standard 658 bp mitochondrial COI barcode was obtained for 32 species, including 14 species never previously barcoded. Neighbor-Joining analyses confirmed the monophyly for all of them. However, comparisons with available sequences from the mainland Europe showed that 4 species presented a significant intraspecific divergence (>3%) whereas a reduced interspecific divergence was found for another set of species.Complementary studies are therefore necessary to better assess their taxonomic levels. Three species -Euscelis lineolata (Cicadellidae), Philaenus spumarius (Aphrophoridae) and Latilica maculipes (Issidae) -account for up to 74.3% of the total insects collected. Although phylogenetic and taxonomic interpretations using restricted sampling and COI sequences alone are not robust, the proportion of new sequences highlight the global lack of knowledge about the Auchenorrhyncha as a whole and the potentially presence of cryptic taxa in different clades. 2 Résumé. Diversité et variations temporelles de la faune Auchenorrhynque dans la région Ajacienne (Corse). Un suivi de la faune Auchenorrhynque dans un maquis de la région d'Ajaccio (Corse) effectué durant une année a permis l'enregistrement de 37 espèces, incluant 3 espèces introduites en Europe ces dernières décennies. Le fragment standard de 658 pb du gene mitochondrial COI utilisé pour le barcoding a été obtenu pour 32 espèces, parmi lesquelles 14 ont été nouvellement séquencées. La monophylie de chacune des espèces a été confirmée par les analyses en Neighbor-Joining. Dans le cas de 4 espèces, les séquences obtenues pour les spécimens de Corse présentent une divergence intraspécifique significative (>3%) par rapport aux séquences de spécimens d'Europe continentale; au contraire, une faible divergence interspécifique a été mise en évidence pour un autre groupe d'espèces. Des études complémentaires sont cependant nécessaires afin de clarifier le statut taxonomique de chacune de ces entités. Trois espèces -Euscelis lineolata (Cicadellidae), Philaenus spumarius (Aphrophoridae) et Latilica maculipes (Issidae)représentent à elles-seules 74.3% des insectes collectés. Bien que les interprétations phylogénétiques et taxonomiques basées sur un échantillonage limité et sur un unique gène mitochondrial ne sont pas robustes, la proportion de séquences nouvelles et la présence d'entités génétiques divergentes mettent en évidence le manque global de connaissances concernant les Auchenorrhynques.

Genetic and endosymbiotic diversity of Greek populations of Philaenus spumarius, Philaenus signatus and Neophilaenus campestris, vectors of Xylella fastidiosa

“…In addition, a phylogenetic tree containing the Greek COI haplotypes of N. campestris and available COI sequences of Neophilaenus sp. (Supplementary Table S3 ) 12 , 91 was constructed using the BI method implemented in MRBAYES v.3.2.1 89 . Based on the Akaike Information Criterion (AIC) as implemented in MEGA v. 10.0.5 90 , the HKY (Hasegawa-Kishino-Yano) model was chosen as the most statistically appropriate model for the data.…”

Species Separation within, and Preliminary Phylogeny for, the Leafhopper Genus Anoscopus with Particular Reference to the Putative British Endemic Anoscopus duffieldi (Hemiptera: Cicadellidae)

“…Some Canadian Anoscopus spp. have been barcoded previously based on specimens collected in Canada and Corsica [ 59 , 60 ], but here we used DNA barcoding for the first time to separate all the known species of Anoscopus in the UK, with unexpected results.…”

“…Anoscopus assimilis has been reported from meadows, pastures and undergrowth of mixed forests at low to montane elevations of the western Mediterranean region, and its distribution seems to extend in western France as far north as to Brittany [ 20 , 22 , 63 ]. We were able to download and examine two identical CO1 sequences thought to be A. assimilis collected in Corsica [ 60 ] (GenBank accession numbers MK816310 and MK188564), but these were acquired from females and hence, the authors acknowledge, impossible to accurately identify morphologically. They shared an identical haplotype with both A. duffieldi and A. albifrons (h1 in Figure 2 ).…”

PM 3/82 (2) Inspection of places of production for Xylella fastidiosa

“…Cicadidae and Tibicinidae species in the EPPO region should also be considered potential vectors (EFSA, 2019a), although Cornara et al (2020) found no evidence for a role of Cicadas in the epidemiology of X. fastidiosa . EFSA (2019a) lists potential European vectors drawn from the Fauna Europaea database (de Jong, 2013, see also Albre & Gibernau, 2019; Morente et al , 2018a,b).…”