Diversity and structural characteristics of soil microbial communities in different habitats of wild Lilium regale Wilson in Wenchuan area

Abstract

Lilium regale Wilson ( L.regale ), originated in the Minjiang River basin in Sichuan, China, has different phenotypic characteristics in different environments. To analyze the correlation between the phenotypes of L.regale and its soil micro-ecological environment, wild habitat soil of L.regale at the two altitudes were selected to analyze the diversity and community structure of microorganisms in soil, and measure the soil physicochemical factors and enzyme activities. The structural composition and diversity of fungal and bacterial communities in hillside and valley soils were significantly different ( p < 0.01). Soil available potassium (AK) and soil enzyme activities such as urease (S_UE), sucrase (S_SC), and catalase (S_CAT) differed significantly different between hillsides and valleys ( p < 0.01), while organic matter (OM), total phosphorus (TP), and polyphenol oxidase (S_PPO) had no great variances. Correlation analysis was conducted between the common and differential microorganisms and the morphological characteristics, soil physicochemical factors and soil enzyme activities of L.regale in both hillside and valley. The results showed that both of the fungal and bacterial could be clustered into two distinct groups by positive and negative correlations, suggesting that the representative microorganism may have structural characteristics that are directly related to soil physicochemical properties and enzyme activities, which conversely affect the phenotype of Lily. Therefore, the study on the native species of horticultural plants and the local soil microhabitat environment will benefit the conservation of wild Lily and provide theoretical guidance for the domestication and breeding of horticultural plants.

Integrated multi-omics investigation revealed the importance of phenylpropanoid metabolism in the defense response of Lilium regale Wilson to fusarium wilt

“…In Z. mays , ZmCCoAOMT2 and ZmCAD , which are involved in lignin biosynthesis, were confirmed to encode components of the QTR mechanism protecting against leaf diseases [ 28 , 29 ]. As a highly resistant wild lily, L. regale is valuable for breeding disease-resistant lily varieties [ 30 ]. Although previous research has identified several WRKY TFs that positively regulate L. regale resistance to fusarium wilt, the QTR mechanism has not been thoroughly investigated.…”

Structural characteristics and diversity of the rhizosphere bacterial communities of wild Fritillaria przewalskii Maxim. in the northeastern Tibetan Plateau

“…Soil samples were collected from 12 areas within the range of wild F. przewalskii between late July and early August, including Weiyuan County (WY), Linxia County (LX), Diebu County (DB), Ruoergai County (RG), Ganzi Tibetan Autonomous Prefecture (GZ), Shiqu County (SQ), Luhuo County (LH), Zeku County (ZK), Yushu City (YS), Zaduo County (ZD), Maqin County (MQ), and Ledu District (LD) (Figure 1). At each site, five F. przewalskii plants were randomly selected by five-point sampling method (Xie et al, 2021). We only chose plants that had grown for more than 3 years.…”

“…We found that the specific distribution at the genus level was a major factor contributing to the differences of rhizosphere bacterial communities among sites (Figure 6A). When the relative abundance greater than 1% in each sample, we classified the common genera (Xie et al, 2021). In total, there were 9 common genera: Gp4 (2.57-12.59%), Spartobacteria (1.02-6.46%), Pseudomonas (1.08-5.27%), Gp6 (1.70-5.89%), Aridibacter (1.02-4.37%), Chryseolinea (1.12-3.98%), Saccharibacteria (1.23-3.08%), Nitrospira (1.23-3.39%), and Terrimonas (1.07-2.69%) (Figure 6B).…”

“…Host plant associated soil microhabitats influence rhizosphere microorganism enrichment (Xiong and Lu, 2022). For example, in recent studies in lilies the rhizosphere microbial community composition differed significantly in different habitats (Xie et al, 2021). This can be explained by the fact that root secretions and soil physicochemical properties vary in different microhabitats, thus selectively assembling specific microbiomes.…”