Diversity and seasonal changes in lactic acid bacteria in the intestinal tract of cultured freshwater fish

Hagi, Tatsuro; Tanaka, Daichi; Iwamura, Yasutada; Hoshino, Takayuki

doi:10.1016/j.aquaculture.2004.01.018

Cited by 142 publications

(130 citation statements)

References 25 publications

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“…In our study, both traditional culturedependent and culture-independent molecular techniques were used to investigate the composition of the autochthonous microbial communities of C. idellus. Our results showed total viable counts In general, these results are consistent with those reported by Hagi, Tanaka, Lwamura and Hoshino (2004), who observed total viable counts from the intestinal tracts of silver carp (Hypophthalmichthys molitrix), common carp (Cyprinus carpio) and crucian carp (Carassius cuvieri) of approximately 1.6 9 10 8 , 1.9 9 10 9 and 5.2 9 10 8 CFU g À1 respectively. These values were slightly higher than those reported by Kim et al (2007) and Wu et al (2010), who detected bacterial numbers from the mucosa of rainbow trout (Oncorhynchus mykiss) and yellow catfish (Pelteobagrus fulvidraco) of 3.0 AE 4.6 9 10 6 and 2.1 9 10 7 CFU g À1 respec-…”

Section: Discussionsupporting

confidence: 92%

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Zhong

Wirth

et al. 2014

Aquac Res

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Traditional culture-based technique and 16S rDNA sequencing method were used to investigate the mucosa-associated autochthonous microbiota of grass carp (Ctenopharyngodon idellus). Twenty-one phylotypes were detected from culturable microbiota, with Aeromonas, Shewanella, Lactococcus, Serratia, Brevibacillus, Delftia, Pseudomonas, Pantoea, Enterobacter, Buttiauxella and Yersinia as their closest relatives. Genomic DNA was directly extracted from the gut mucosa of C. idellus originating from six different geographical regions, and used to generate 609 random bacterial clones from six clone libraries and 99 archaeal clones from one library, which were grouped into 67 bacterial and four archaeal phylotypes. Sequence analysis revealed that the intestinal mucosa harboured a diversified bacterial microbiota, where Proteobacteria, Firmicutes and Bacteroidetes were dominant, followed by Actinobacteria, Verrucomicrobia and DeinococcusThermus. The autochthonous bacterial communities in the gut mucosa of fish from different aquatic environments were not similar (C s < 0.80), but c-Proteobacteria was a common bacterial class. In comparison to bacterial communities, the archaeal community obtained from one library consisted of Crenarchaeota and Euryarchaeota. These results demonstrate that molecular methods facilitate culture-independent studies, and that fish gut mucosa harbours a larger bacterial diversity than previously recognized. The grass carp intestinal habitat selects for specific bacterial taxa despite pronounced differences in host environments.

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Section: Discussionsupporting

confidence: 92%

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Zhong

Wirth

et al. 2014

Aquac Res

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“…Populations of lactic acid bacteria (LAB) are highly variable in the intestines of fish and change as the aquatic environment does, i.e., farming or wilderness (Hagi et al, 2004). LAB is a group widely investigated in animals because it plays an important role on the health and nutrition of the host (Vázquez et al, 2005;Lauzon & Ringø, 2012).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the intestinal microbiota of wild caught and farmed fine flounder (Paralichthys adspersus)

Salas‐Leiva

Opazo

Remond

et al. 2017

lajar

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ABSTRACT.A comparative analysis of the cultivable intestinal microbiota of farmed (AF) and wild-caught (WF) Paralichthys adspersus was performed. The 16S rRNA gene was used for taxa identification, and the ITS region for strain differentiation. We detected the presence of Vibrio, Bacillus, Photobacterium, Staphylococcus and Carnobacterium in AF, and Exiguobacterium, Klebsiella, Arthrobacter, Raoultella, Kluyvera, Myroides, Streptococcus, Vagococcus, Staphylococcus, Acinetobacter, Psychrobacter, Lactobacillus, Weissella and Lactococcus in WF. The microbial community was more diverse in WF than in AF. Some bacterial groups were only found in wild-caught fish and may be studied as potential beneficial agents for improving production traits in farmed fish. As the first study of microbiota of P. adspersus, it provides significant information that can potentially help improve farming practices by using strains as species-specific probiotics.

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“…Authors reported that total viable counts on Tryptone soya agar varied from 3 to 8 log CFU/g and are in correlation with total microscopic counts after DAPI straining, indicating that cultivable microorganisms dominated in the microflora. Total viable counts determined by Hagi et al (2004) in the intestinal tract of four freshwater fish species were less variable (6-9 log CFU/g) and dependent on water temperature. LAB counts varied between 5 and 7 log CFU/g but lactobacilli were determined only in one sample of Carassius cuvieri (Temminck et Schlegel, 1846).…”

Section: Discussionmentioning

confidence: 92%

“…LAB seem to be good probiotic candidates for fish because they are naturally present in fish gut. Genera of LAB often isolated from fish include Lactobacillus, Lactococcus, Enterococcus, Leuconostoc, Carnobacterium, Enterococcus and Vagococcus (González et al 2000;Hagi et al 2004;Perez-Sanchez et al 2011). Also, LAB of non-fish origin were tested as probiotics in aquaculture (Kesarcodi-Watson et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of bifidobacteria and lactobacilli in digestive tract of some freshwater fishes

et al. 2012

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Distal parts of the fish intestine were analyzed for presence of bifidobacteria and lactobacilli using selective agars. Seventy seven samples from Cyprinus carpio, Oncorhynchus mykiss, Carassius auratus, Tinca tinca, Perca fluviatilis, Rutilus rutilus, Scardinius erythrophthalmus, Oreochromis niloticus, and Squalius cephalus were collected randomly throughout years 2008 and 2009. Bifidobacteria were detected in 5 samples from 4 fish species at counts 2.18-4.29 log CFU/g, lactobacilli were present in 6 fish species at counts 1.21-3.65 log CFU/g. Seven bifidobacterial isolates were identified to the species level using biochemical tests and by sequencing of 16S rRNA gene. Three strains belonged to species B. longum, two isolates were identified as B. dentium, one strain as B. asteroides and one isolate was not determined to the species level by employed methods. As identified bifidobacterial species are considered to be of human, animal or honeybee origin, they probably derived as contamination from sewage or other sources. After further more detail testing, the possible use of isolated bifidobacteria as probiotics is promising since they were able to pass through the digestive tract successfully.

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Diversity and seasonal changes in lactic acid bacteria in the intestinal tract of cultured freshwater fish

Cited by 142 publications

References 25 publications

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Diversity of autochthonous bacterial communities in the intestinal mucosa of grass carp (Ctenopharyngodon idellus) (Valenciennes) determined by culture-dependent and culture-independent techniques

Characterization of the intestinal microbiota of wild caught and farmed fine flounder (Paralichthys adspersus)

Occurrence of bifidobacteria and lactobacilli in digestive tract of some freshwater fishes

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