Diversity and prevalence of Brazilian bipartite begomovirus species associated to tomatoes

Fernandes, Fernanda Rausch; Albuquerque, Leonardo Cunha de; Giordano, Leonardo de B.; Boiteux, L. S.; Ávila, A. C. de; Inoue‐Nagata, Alice K.

doi:10.1007/s11262-007-0184-y

Cited by 85 publications

(116 citation statements)

References 26 publications

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“…An example is the introduction of the B-biotype (B. tabaci) in Brazil which was responsible of vectoring of viruses present in native plants only onto cultured tomato crops, leading to new virus diseases in this crop (Fernandes et al 2008). Whether antagonists will also extend their ranges, thereby following the herbivores, is unknown for the moment.…”

Section: Insectsmentioning

confidence: 99%

Robust cropping systems to tackle pests under climate change. A review

Lamichhane¹,

Barzman²,

Booij³

et al. 2014

Agron. Sustain. Dev.

123

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Agriculture in the twenty-first century faces the challenge of meeting food demands while satisfying sustainability goals. The challenge is further complicated by climate change which affects the distribution of crop pests (intended as insects, plants, and pathogenic agents injurious to crops) and the severity of their outbreaks. Increasing concerns over health and the environment as well as new legislation on pesticide use, particularly in the European Union, urge us to find sustainable alternatives to pesticide-based pest management. Here, we review the effect of climate change on crop protection and propose strategies to reduce the impact of future invasive as well as rapidly evolving resident populations. The major points are the following: (1) the main consequence of climate change and globalization is a heightened level of unpredictability of spatial and temporal interactions between weather, cropping systems, and pests; (2) the unpredictable adaptation of pests to a changing environment primarily creates uncertainty and projected changes do not automatically translate into doom and gloom scenarios; (3) faced with uncertainty, policy, research, and extension should prepare for worst-case scenarios following a "no regrets" approach that promotes resilience vis-à-vis pests which, at the biophysical level, entails uncovering what currently makes cropping systems resilient, while at the organizational level, the capacity to adapt needs to be recognized and strengthened; (4) more collective approaches involving extension and other stakeholders will help meet the challenge of developing more robust cropping systems; (5) farmers can take advantage of Web 2.0 and other new technologies to make the exchange of updated information quicker and easier; (6) cooperationThe views expressed by Stephen R. H. Langrell are purely his own and may not in any circumstances be regarded as stating an official position of the European Commission.

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Section: Insectsmentioning

confidence: 99%

Robust cropping systems to tackle pests under climate change. A review

Lamichhane¹,

Barzman²,

Booij³

et al. 2014

Agron. Sustain. Dev.

123

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“…O gênero Begomovirus é constituído por espécies em que o genoma possui uma ou duas moléculas de DNA circular de fita simples, como é o caso da espécie com genoma bipartido Tomato yellow vein streak virus (TYVSV) (Matos et al, 2003), Tomato severe rugose virus (TSRV) e Tomato mottle leaf curl virus (ToMLCV), que são espécies encontradas no Brasil (Fernandes et al, 2008).…”

Section: Introductionunclassified

Capacidade combinatória de híbridos de tomateiro de crescimento determinado, resistentes a Begomovirus e Tospovirus

Raphael

Pádua

Gomes

et al. 2010

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar a capacidade combinatória de linhagens de tomateiro com hábito de crescimento determinado, com resistência múltipla a espécies dos gêneros Begomovirus e Tospovirus, e identificar combinações híbridas superiores. O experimento foi realizado em casa de vegetação, com 14 híbridos obtidos do cruzamento de sete linhagens femininas (grupo I) com duas linhagens masculinas (grupo II), em um dialelo parcial. As seguintes características agronômicas foram avaliadas: produção total, produção precoce, massa média de frutos, formato, firmeza inicial e firmeza na meia-vida. As linhagens genitoras TOM-680 e TOM-682, do grupo I, se destacaram por exibir as maiores estimativas de capacidade geral de combinação (CGC) quanto às características produção total, produção precoce e massa média de frutos, enquanto a linhagem TOM-585 se destacou quanto aos maiores valores de produção total e massa média de frutos. No grupo II, a linhagem TOM-698 apresentou estimativas superiores de CGC para as características de produção total, produção precoce, massa média dos frutos e firmeza inicial dos frutos. O híbrido TOM-682xTOM-698 apresenta as maiores estimativas de capacidade geral e específica de combinação para produção total, produção precoce e meia-vida da firmeza, e é o genótipo mais promissor entre os materiais testados.Termos para indexação: Solanum lycopersicon, análise dialélica, geminivírus, melhoramento, resistência. Combining ability of determinate-growth-habit tomato hybrids resistant to Begomovirus and TospovirusAbstract -The objective of this work was to evaluate the combining ability of determinate-growth-habit tomato inbred lines with multiple resistance to Tospovirus and Begomovirus species, ant to identify superior hybrid combinations. The experiment was carried out in greenhouse, with 14 hybrids obtained from crosses of seven lines of the group I (female parents) with two lines of the group II (male parent), in a partial diallel.The following agronomical traits were assessed: total production, early production, mean fruit mass, shape, initial firmness and half life firmness. The parental lines TOM-680 and TOM-682, from group I, stand out with the largest general combining ability (GCA) concerning total production, early production and average fruit mass, while line TOM-585 stood out as to higher values of total production and average fruit mass. In group II, TOM-698 showed higher estimates of GCA as to total production, early production, average fruit mass and initial consistence of the fruits. The hybrid TOM-682xTOM-698 had the highest estimate of general and specific combining ability to total production, early production and average fruit mass, and it is the most promising genotype among the materials tested.

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“…PCR analysis using the universal primers suggested by Rojas et al (1993), particularly the primer pair pARc715 and pALv1978, enables a comparison of this terminal cp 5' end, the intergenic region and the terminal rep 5' end. This analysis has been used for diversity study on Brazilian begomovirus (Faria & Maxwell, 1999;Ribeiro et al, 2003;Fernandes et al, 2008). Thus far, it has only been possible after sequencing analysis of the amplified DNA fragment.…”

mentioning

confidence: 99%

“…Nucleotide sequence of PCR products after reaction using primers pARc715 and pALv1978 (Rojas et al, 1993) of many tomato samples were analyzed (Fernandes et al, 2008). Nine ToSRV-like sequences were randomly selected and aligned with six ToYVSV-like sequences using ClustalW (Thompson et al,1994), one ToCMoV sequence (NC003664), one ToYVSV sequence (AY751742), one ToSRV (AY029750) sequence and one ToRMV sequence (AF291705).…”

mentioning

confidence: 99%

“…In order to validate the primers, fifty begomovirus isolates were selected, which were preliminarily identified by PCR product direct sequencing (Fernandes et al, 2008). They all shared a partial genome sequence higher than 90% to either ToSRV or ToYVSV, thus being preliminarily classified as ToSRV-like (34 isolates) and ToYVSV-like (16 isolates) viruses, respectively.…”

mentioning

confidence: 99%

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Development of a species-specific detection method for three Brazilian tomato begomoviruses

Fernandes¹,

Albuquerque²,

Inoue‐Nagata³

2010

Trop. plant pathol.

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The begomoviruses (fam. Geminiviridae) have an ssDNA genome, are transmitted by whiteflies, and cause significant losses in tomato fields in Brazil. Nowadays, begomovirus species identification is carried out through the analysis of their complete genome sequence. Due to the high costs and difficulty in applying this technique to large-scale analysis, we aimed to develop a species-specific detection method based on PCR. Three species were targeted for amplification: Tomato severe rugose virus (ToSRV), Tomato rugose mosaic virus (ToRMV) and Tomato yellow vein streak virus (ToYVSV). Thirteen primer combinations were designed, and four were finally selected and tested against a group of 82 samples, including cloned DNA and viral DNA from infected plants previously identified by direct sequencing of PCR products. Three primer combinations were selected that could distinguish the three species, and confirmed by sequencing of amplified products. These combinations were validated by evaluation of field-collected tomato samples infected by begomovirus and this demonstrated that PCR can be a useful tool to distinguish the viruses and detect mixed infections caused by three begomovirus species. Keywords: Solanum lycopersicum, PCR detection, geminivirus, ToSRV, ToYVSV. RESUMO Desenvolvimento de método de detecção específico à espécie para três begomovirus no BrasilOs begomovírus (fam. Geminiviridae) possuem um genoma de ssDNA, são transmitidos por mosca-branca, e causam danos importantes em campos de produção de tomate no Brasil. Atualmente, a identificação de espécies de begomovírus é realizada pela análise da seqüência completa do genoma. Devido ao alto custo e dificuldade em se aplicar essa técnica para análises em larga escala, o objetivo desse trabalho foi desenvolver um método de detecção espécie-específico baseado em PCR. Três espécies foram avaliadas para amplificação: Tomato severe rugose virus (ToSRV), Tomato rugose mosaic virus (ToRMV) e Tomato yellow vein streak virus (ToYVSV). Foram testadas treze combinações de primers, e quatro foram selecionadas e avaliadas em um grupo de 82 amostras, incluindo DNA clonado e DNA viral total extraído de plantas infectadas com begomovírus previamente identificados por seqüenciamento direto de produto de PCR. Três combinações de primers capazes de distinguir as três espécies foram selecionadas, e confirmadas por seqüenciamento dos produtos amplificados. Estas combinações foram validadas com amostras de tomate infectadas por begomovírus em campo, demonstrando que a PCR pode ser uma ferramenta útil para separar as espécies virais e detectar infecções mistas causadas pelas três espécies de begomovírus.

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Diversity and prevalence of Brazilian bipartite begomovirus species associated to tomatoes

Cited by 85 publications

References 26 publications

Robust cropping systems to tackle pests under climate change. A review

Robust cropping systems to tackle pests under climate change. A review

Capacidade combinatória de híbridos de tomateiro de crescimento determinado, resistentes a Begomovirus e Tospovirus

Development of a species-specific detection method for three Brazilian tomato begomoviruses

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