Diversity and Plasticity of Sex Determination and Differentiation in Fishes

Kobayashi, Yasuhisa; Nagahama, Yoshitaka; Nakamura, Masaru

doi:10.1159/000342009

Cited by 238 publications

(182 citation statements)

References 183 publications

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“…The diversity and plasticity of the sex-determining mechanisms in the animal kingdom have long been appreciated (30,31). In vertebrates, sex can be determined genetically by different systems of sex chromosomes (including XY and ZW) or through environmental cues such as temperature or social situation.…”

Section: Discussionmentioning

confidence: 99%

Structure–function analysis of mouse Sry reveals dual essential roles of the C-terminal polyglutamine tract in sex determination

Zhao

Davidson

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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The mammalian sex-determining factor SRY comprises a conserved high-mobility group (HMG) box DNA-binding domain and poorly conserved regions outside the HMG box. Mouse Sry is unusual in that it includes a C-terminal polyglutamine (polyQ) tract that is absent in nonrodent SRY proteins, and yet, paradoxically, is essential for male sex determination. To dissect the molecular functions of this domain, we generated a series of Sry mutants, and studied their biochemical properties in cell lines and transgenic mouse embryos. Sry protein lacking the polyQ domain was unstable, due to proteasomal degradation. Replacing this domain with irrelevant sequences stabilized the protein but failed to restore Sry's ability to up-regulate its key target gene SRY-box 9 (Sox9) and its sex-determining function in vivo. These functions were restored only when a VP16 transactivation domain was substituted. We conclude that the polyQ domain has important roles in protein stabilization and transcriptional activation, both of which are essential for male sex determination in mice. Our data disprove the hypothesis that the conserved HMG box domain is the only functional domain of Sry, and highlight an evolutionary paradox whereby mouse Sry has evolved a novel bifunctional module to activate Sox9 directly, whereas SRY proteins in other taxa, including humans, seem to lack this ability, presumably making them dependent on partner proteins(s) to provide this function.sex development | Y chromosome | testis | TESCO

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Section: Discussionmentioning

confidence: 99%

Structure–function analysis of mouse Sry reveals dual essential roles of the C-terminal polyglutamine tract in sex determination

Zhao

Davidson

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…Sex dimorphic growth pattern has been found in many species of teleost fish (Gui 2007;Mei and Gui 2014), and the significant growth difference between female and male and its application implications have been noted in some farmed fish (Taranger et al 2010;Gui and Zhou 2010;Gui and Zhu 2012;Kobayashi et al 2013;Liu et al 2013a). Yellow catfish (Pelteobagrus fulvidraco), one of the important freshwater fish species in Asia, has been widely cultured for its delicious flesh and high nutritive value (Huang et al 1999).…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Transcriptome Provides Candidate Genes for Sex Determination/Differentiation and SSR/SNP Markers in Yellow Catfish

Chen

Mei

et al. 2014

Mar Biotechnol

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Sex dimorphic growth pattern has significant theory and application implications in fish. Recently, a Y-and Xspecific allele marker-assisted sex control technique has been developed for mass production of all-male population in yellow catfish (Pelteobagrus fulvidraco), but the genetic information for sex determination and sex control breeding has remained unclear. Here, we attempted to provide the first insight into a comprehensive transcriptome covering multiple tissues from XX females, XY males, and YY super-males of yellow catfish by using 454 GS-FLX platform, for a better assembly and gene coverage. A total of 1,202,933 high quality reads (about 540 Mbp) were obtained and assembled into 28,297 contigs and 141,951 singletons. BLASTX searches against the NCBI non-redundant protein database (nr) led a total of 52,564 unique sequences including 18,748 contigs and 33,816 singletons to match 25,669 known or predicted unique proteins. All of them with annotated function were categorized by gene ontology (GO) analysis, and 712 were assigned to reproduction and reproductive process. Some potential genes relevant to reproductive system including steroid hormone biosynthesis and GnRH (gonadotropin-releasing hormone) signaling pathway were further identified by Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis; and at least 21 sex determination and differentiation-related genes, such as Dmrt1, Sox9a/b, Cyp19b, WT1, and AMH were identified and characterized. Additionally, a total of 82,794 simple sequence repeats (SSRs), 26,450 single nucleotide polymorphisms (SNPs), and 4,145 insertions and deletions (INDELs) were revealed from the transcriptome data. Therefore, the current transcriptome resources highlight further studies on sex-control breeding in yellow catfish and will benefit future studies on reproduction and sex determination in teleost fish.

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“…Once determined, the sex of an individual remains the same for the remainder of the life. However, some fishes can change their sexes naturally (Devlin and Nagahama 2002;Frisch 2004;Kobayashi et al 2013). As sex change in such hermaphroditic fishes involves gonadal reconstruction after reaching adulthood, they serve as good animal models for the investigation of gonadal sex determination/differentiation in vertebrates.…”

Section: Introductionmentioning

confidence: 99%

Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse

et al. 2014

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Doublesex-mab 3-related transcription factor 1 (DMRT1) has been identified as the first conserved gene involved in the testicular differentiation of vertebrates. However, the precise role of DMRT1 in spermatogenesis has not been made clear. In this study, immunohistochemical method was used to observe DMRT1 protein localization in order to resolve cellular profile of DMRT1 in the adult testis of three-spot wrasse. DMRT1 protein was clearly and specifically localized in the Sertoli cells of all spermatogenic cells and epithelial cells comprising the efferent duct, but not in the germ cells. In addition, adult males were treated with aromatase inhibitor (AI) for investigating the role of estrogen on the transcription of DMRT1. AI treatment caused an increase in the levels of DMRT1 transcripts in the efferent duct region, concomitant with a decrease in spermatogonia and spermatocytes.Keywords DMRT1 Á Spermatogenesis Á Efferent duct Á Wrasse Á Aromatase inhibitor Á Sertoli cell Abbreviations DMRT1 Doublesex-mab 3-related transcription factor 1 E2

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Diversity and Plasticity of Sex Determination and Differentiation in Fishes

Cited by 238 publications

References 183 publications

Structure–function analysis of mouse Sry reveals dual essential roles of the C-terminal polyglutamine tract in sex determination

Structure–function analysis of mouse Sry reveals dual essential roles of the C-terminal polyglutamine tract in sex determination

A Comprehensive Transcriptome Provides Candidate Genes for Sex Determination/Differentiation and SSR/SNP Markers in Yellow Catfish

Histological observation of doublesex-mab 3-related transcription factor 1 (DMRT1) localization in the adult testis of three-spot wrasse

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