Diversity and Plant Growth-Promotion of Endophytic Fungi Isolated from the Roots of Plants in Dokdo Islands

Abstract

Endophytic fungi were isolated from the roots of plants growing naturally on the island of Dokdo.Plant samples, such as Miscanthus sinensis, Achyranthus japonica and Echinochloa crusgali were isolated from Dongdo, and those such as Honkenya peploides and Artemsia koidzumii were isolated from Seodo.Twenty one strains of endophytic fungi were isolated from these plants. To identify the strains, PCR (polymerase chain reaction) amplification of the partial ITS (Internal Transcribed Spacer) regions was done with universal primers ITS-1 and ITS-4 to determine the nucleotide sequence of the ITS regions. (19%). As a result of bioassay, Ec-3-1 strain isolated from Echinochloa crusgalli showed plant growth-promotion activity. Of all the endophytic fungi isolated, Penicillium sp. was the most abundantly distributed fungal strain in all plants used in this study.

Diversity of Endophytic Fungi from the Roots of Halophytes Growing in Go-chang Salt Marsh

“…그리고 염생식물과 공생관계 에 있으며, 내염성을 가질 수 있도록 영향을 주는 것이 알 려져 있다 (Rodriguez et al, 2004;Rodriguez et al, 2008 (Vazquez et al, 2000;Yamada et al, 2001). 그리고 배양 된 염생식물 뿌리의 끝 단면에서 내생진균을 potato dextrose agar (PDA)에 계대배양하여 순수 분리하였다 (Khan et al, 2008;Kil et al, 2009;You et al, 2011). 염 생식물의 뿌리에서 순수 분리한 내생진균들을 배양하기 위 하여 potato dextrose broth (PDB)에서 7~14일 동안 100 rpm으로 25…”

“…그리고 templete 조건은 10 mM Tris-HCl(pH 8. You et al, 2011). 그리고 증폭된 PCR 산물은 1.5% agarose gel에서 전기영동 후, ethidium bromide (EtBr)을 사용하여 10분 염색 후, UV transilluminator로 전기영동 밴드를 확인 하였다 (You et al, 2011 (Tamura et al, 2007).…”

Gibberellin A4Producted by Fusarium solani Isolated from the Roots of Suaeda japonica Makino

“…그리고 GA는 diterpenoid 복합체이고 식물의 생장, 종자의 발아, 줄기신장, 개화촉진, 과육의 성숙 등을 조절한다고 알려져 있다 [5,10,11,28]. GA는 G. fujikuroi 외에도 Phaeosphaeria sp.…”

Genetic Diversity of Culturable Endophytic Fungi Isolated from Halophytes Naturally Growing in Muan Salt Marsh

“…식물시료는 염습지 자생식물인 해홍나물 (Suaeda maritima), 갯질경(Limonium tetragonum), 칠면초 [19,21]. 그리고 potato dextrose agar (PDA)에 계대배양하 여 순수 분리하였다 [8,10,22].…”