Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species

Li, A.-H.; Yuan, Feng; Groenewald, Marizeth; Bensch, Konstanze; Yurkov, Andrey; Li, K.; Han, Pei‐Jie; Guo, Liangdong; Aime, M. Catherine; Sampaio, José Paulo; Jindamorakot, Sasitorn; Turchetti, Benedetta; Inácio, João; Fungsin, Bundit; Wang, Q.-M.; Bai, Feng‐Yan

doi:10.1016/j.simyco.2020.01.002

Cited by 103 publications

(161 citation statements)

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“…Interestingly, repeat testing by Vitek2 at the central laboratory still yielded correct identification for only 18 of the 42 isolates, suggesting the ability of phenotypic methods to misidentify even common yeast pathogens on some occasions [35]. The Vitek2 also misidentifies Papiliotrema aspenensis as P. laurentii [36, 37].…”

Section: Discussionmentioning

confidence: 99%

Molecular identification, genotypic heterogeneity and comparative pathogenicity of environmental isolates of Papiliotrema laurentii

Asadzadeh

Ahmad

Khan

et al. 2020

Journal of Medical Microbiology

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Introduction. Papiliotrema laurentii, formerly Cryptococcus laurentii, is typically isolated from environmental sources, but also occasionally from clinical specimens. Other close relatives may be misidentified as P. laurentii by phenotypic methods. P. laurentii usually lacks melanin; however, melanin-forming strains have also been isolated. Hypothesis/Gap Statement. Although melanin production by encapsulated budding yeasts is considered a major virulence factor, the comparative pathogenicity of melanin-forming and non-melanized environmental strains of P. laurentii has rarely been studied. Aim. We performed phenotypic and molecular identification and determined the genotypic heterogeneity among P. laurentii isolates. We also studied the pathogenicity of melanin-forming and non-melanized strains in normal and immunosuppressed mice. Methodology. Eleven environmental isolates were tested for their identity by Vitek2 and/or ID32C systems, and by PCR-sequencing of the internal transcribed spacer (ITS) region and D1/D2 domains of ribosomal DNA (rDNA). Genotypic heterogeneity was studied by sequence comparisons. The pathogenicity of melanized and non-melanized P. laurentii strains was studied in intravenously infected normal and immunosuppressed BALB/c mice. Results. Phenotypic methods identified seven of the environmental isolates, while PCR-sequencing of the ITS region and D1/D2 domains of rDNA detected two and five isolates, respectively, as P. laurentii. Sequence comparisons demonstrated genotypic heterogeneity among P. laurentii. The remaining four environmental isolates yielded expected results. None of the normal mice infected with 105 cells of melanized/non-melanized P. laurentii strains died. Infection of immunosuppressed mice with 107 cells caused higher mortality with non-melanized P. laurentii, while viable counts in brain/lung tissue were higher in mice infected with a melanized strain and were detectable for up to 14 days. Conclusion. Phenotypic methods lacked specificity, but PCR-sequencing of D1/D2 domains correctly identified P. laurentii and sequence comparisons demonstrated the genotypic heterogeneity of the isolates. Both melanized and non-melanized strains at a higher dose caused mortality in immunosuppressed mice and persisted in brain/lung tissue up to 14 days post-infection.

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Section: Discussionmentioning

confidence: 99%

Molecular identification, genotypic heterogeneity and comparative pathogenicity of environmental isolates of Papiliotrema laurentii

Asadzadeh

Ahmad

Khan

et al. 2020

Journal of Medical Microbiology

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“…nov. can be distinguished from its closest relative, T. tronadorensis, not only on the basis of the D1/D2 region of the LSU rRNA and the ITS region but also by various phenotypic characteristics as shown in Table 1. Members of the genus Teunia have been found in diverse habitats: T. korlaensis, the type species of the genus Teunia, was proposed based on a strain isolated from soil collected in Xinjiang province, PR China [1]; T. globosa was obtained from a leaf of an unidentified plant collected in Lulang, Tibet [1]; and T. helanensis was found in soil collected from Helan Shan (the Helan Mountains), China [1]. Furthermore, two closely related species, T. cuniculi and T. tronadorensis, were proposed based on strains isolated from a rabbit's faecal sample collected in Korea [26] and from glacial melt-water in Argentina [2], respectively.…”

Section: Phenotypic Characterizationmentioning

confidence: 99%

“…Consequently, C. cuniculi, F. tronadorensis and F. betulae were transferred to the genus Teunia and renamed as Teunia cuniculi, Teunia tronadorensis and Teunia betulae, respectively. Teunia korlaensis was designated as the type species of this genus [1]. In the seven-gene dataset and rDNA phylogeny, the genus Teunia was located in the Cryptococcaceae (Tremellales, Tremellomycetes, Agaricomycotina and Basidiomycota) and clustered with the genera Cryptococcus and Kwoniella with 96-100 % bootstrap and 1.0 posterior probability supports but still separated from them [1].…”

mentioning

confidence: 98%

“…The genus Teunia was proposed in early 2020 by Li et al [1], based on the results of phylogenetic analysis of a seven-gene dataset. This consisted of the internal transcribed spacer (ITS) region rRNA; the D1/D2 domain of the LSU rRNA; the small subunit (SSU) rRNA; the two subunits of RNA polymerase II (RPB1 and RPB2); the translation elongation factor 1-α (TEF1); and cytochrome b (CYTB) genes.…”

mentioning

confidence: 99%

“…In the seven-gene dataset and rDNA phylogeny, the genus Teunia was located in the Cryptococcaceae (Tremellales, Tremellomycetes, Agaricomycotina and Basidiomycota) and clustered with the genera Cryptococcus and Kwoniella with 96-100 % bootstrap and 1.0 posterior probability supports but still separated from them [1]. However, Li et al [1], when comparing D1/D2 region sequences, noted that more than 30 undescribed or incorrectly identified strains may represent more than 20 species in the Teunia clade. Presently, the genus Teunia contains six described species: T. betulae, T. cuniculi, T. globosa, T. helanensis, T. korlaensis and T. tronadorensis.…”

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confidence: 99%

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Teunia siamensis f.a., sp. nov., a novel tremellaceous yeast species isolated from soil in a secondary peat swamp forest area

Khunnamwong

Lertwattanasakul

Limtong

2020

International Journal of Systematic and Evolutionary Microbiology

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The strain DMKU-XD44, representing an anamorphic novel yeast species, was isolated from soil collected in a peat swamp forest (PSF) area in Rayong Botanical Garden in eastern Thailand. On the basis of sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, Teunia cuniculi CBS 10309T was the most closely related species. The novel species differed from the T. cuniculi type by 2.5 % (14 nucleotide substitutions) in the D1/D2 region of the LSU rRNA gene and by 8.0 % (40 nucleotide substitutions) in the ITS region. The results of a phylogenetic analysis, based on the combined sequences of the ITS region and the D1/D2 region, indicated that DMKU-XD44 represents a member of the Teunia clade in the Cryptococcaceae (Tremellales, Tremellomycetes, Agaricomycotina and Basidiomycota) and is phylogenetically distinct from other species of the genus Teunia in the clade. Therefore, DMKU-XD44 represents a novel species of the genus Teunia. The name Teunia siamensis f.a., sp. nov. is proposed. The holotype is DMKU-XD44, while the MycoBank number is MB 832816.

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Yeasts from tropical forests: Biodiversity, ecological interactions, and as sources of bioinnovation

Rosa,

Lachance,

Limtong

et al. 2023

Yeast

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Tropical rainforests and related biomes are found in Asia, Australia, Africa, Central and South America, Mexico, and many Pacific Islands. These biomes encompass less than 20% of Earth's terrestrial area, may contain about 50% of the planet's biodiversity, and are endangered regions vulnerable to deforestation. Tropical rainforests have a great diversity of substrates that can be colonized by yeasts. These unicellular fungi contribute to the recycling of organic matter, may serve as a food source for other organisms, or have ecological interactions that benefit or harm plants, animals, and other fungi. In this review, we summarize the most important studies of yeast biodiversity carried out in these biomes, as well as new data, and discuss the ecology of yeast genera frequently isolated from tropical forests and the potential of these microorganisms as a source of bioinnovation. We show that tropical forest biomes represent a tremendous source of new yeast species. Although many studies, most using culture‐dependent methods, have already been carried out in Central America, South America, and Asia, the tropical forest biomes of Africa and Australasia remain an underexplored source of novel yeasts. We hope that this review will encourage new researchers to study yeasts in unexplored tropical forest habitats.

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Diversity and phylogeny of basidiomycetous yeasts from plant leaves and soil: Proposal of two new orders, three new families, eight new genera and one hundred and seven new species

Cited by 103 publications

References 67 publications

Molecular identification, genotypic heterogeneity and comparative pathogenicity of environmental isolates of Papiliotrema laurentii

Molecular identification, genotypic heterogeneity and comparative pathogenicity of environmental isolates of Papiliotrema laurentii

Teunia siamensis f.a., sp. nov., a novel tremellaceous yeast species isolated from soil in a secondary peat swamp forest area

Yeasts from tropical forests: Biodiversity, ecological interactions, and as sources of bioinnovation

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