Diversity and pathogenicity of Colletotrichum species causing strawberry anthracnose in Taiwan and description of a new species, Colletotrichum miaoliense sp. nov.

Abstract

Strawberry is a small fruit crop with high economic value. Anthracnose caused by Colletotrichum spp. poses a serious threat to strawberry production, particularly in warm and humid climates, but knowledge of pathogen populations in tropical and subtropical regions is limited. to investigate the diversity of infectious agents causing strawberry anthracnose in taiwan, a disease survey was conducted from 2010 to 2018, and Colletotrichum spp. were identified through morphological characterization and multilocus phylogenetic analysis with internal transcribed spacer, glyceraldehyde 3-phosphate dehydrogenase, chitin synthase, actin, beta-tubulin, calmodulin, and the intergenic region between Apn2 and MAT1-2-1 (ApMAT). Among 52 isolates collected from 24 farms/nurseries in taiwan, a new species, Colletotrichum miaoliense sp. nov. (6% of all isolates), a species not previously known to be associated with strawberry, Colletotrichum karstii (6%), and three known species, Colletotrichum siamense (75%), Colletotrichum fructicola (11%), and Colletotrichum boninense (2%), were identified. The predominant species C. siamense and C. fructicola exhibited higher mycelial growth rates on potato dextrose agar and caused larger lesions on wounded and non-wounded detached strawberry leaves. Colletotrichum boninense, C. karstii, and C. miaoliense only caused lesions on wounded leaves. Understanding the composition and biology of the pathogen population will help in disease management and resistance breeding. Strawberry (Fragaria × ananassa Duch.) is a popular small fruit crop with high economic and nutritive value. Strawberry is in high demand globally. From 2008 to 2018, the annual worldwide cultivation of strawberries increased from approximately 400 to 483 thousand hectares 1. Although strawberries are native to temperate regions, they can also be grown in tropical and subtropical regions (sometimes under high-altitude conditions). The land areas devoted to strawberry cultivation in Colombia,

Biocontrol Ability and Mechanism of a Broad-Spectrum Antifungal Strain Bacillus safensis sp. QN1NO-4 Against Strawberry Anthracnose Caused by Colletotrichum fragariae

“…Among the causal agents, C. fragariae is an important fungus which causes the disease named anthracnose crown rot (Miller-Butler et al, 2018). The fungi can infect the whole strawberry plant containing fruit, and it has become more destructive in the past decade (Chen et al, 2019;Chung et al, 2020). Currently, the control of strawberry anthracnose is done primarily through chemical fungicides to reduce post-harvest losses (Dukare et al, 2019).…”

Characterization of Colletotrichum siamense causing crown rot of strawberry in Jingzhou, Hubei Province

“…Pathogens causing strawberry root and crown rot included Colletotrichum, Phytophthora, Neopestalotiopsis and Macrophomina (Noh et al, 2018;Park et al, 2019;Zhang et al, 2020;Chung et al, 2020). The pathogen of Colletotrichum sp.…”

Morphological and Molecular Characteristics of Fungal Species Associated with Crown Rot of Strawberry in South Korea

“…With the progress in molecular analysis and bioinformatics, the crystal explicit knowledge regarding fungi and their association with the host has increased over the last decade (Chung et al, 2020). The multigene phylogenetic approach uses sophisticated and reliable tools for the classi cation of cryptic fungal taxa and fungi, including plant pathogen.…”