Diversity and natural selection in Plasmodium vivax Duffy binding protein gene

Cole-Tobian, Jennifer L.; King, Christopher L.

doi:10.1016/s0166-6851(02)00327-4

Cited by 97 publications

(134 citation statements)

References 49 publications

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“…To evaluate the association between positive natural selection and host immune evasion, we examined the genetic diversity in predicted or known B- and T-cell epitopes [14] and MHC binding regions [30] in PvDBPII. Most predicted B- and T-cell epitopes and MHC binding regions were polymorphic, and most had a higher rate of non-synonymous mutations ( K n) than that of synonymous mutations ( K s) with the exceptions being the regions within peptides 13 and Ic (Table 3).…”

Section: Resultsmentioning

confidence: 99%

“…To assess the possibility that diversity in PvDBPII within the Myanmar P. vivax isolates may have arisen from host's immune pressure, the genetic diversity in predicted or known B- and T-cell epitopes [14] and MHC binding regions [30] in PvDBPII was examined. Polymorphism of each region was analysed by DnaSP ver.…”

Section: Methodsmentioning

confidence: 99%

“…Critical binding motifs in PvDBPII have been mapped to a 170 amino acid stretch (amino acids 291-460), which includes cysteines 5-8 [11,12]. PvDBPII shows the highest genetic diversity compared to the remaining PvDBP regions and appears to be under strong selective pressure [13,14]. Analysis of genetic variation of PvDBPII among P. vivax field isolates from different geographical regions, including Brazil, Colombia, South Korea, Papua New Guinea, Thailand, showed that the PvDBPII is highly polymorphic, but the cysteine residues are conserved within and between P. vivax populations from different geographic regions [14-21].…”

Section: Introductionmentioning

confidence: 99%

“…PvDBPII shows the highest genetic diversity compared to the remaining PvDBP regions and appears to be under strong selective pressure [13,14]. Analysis of genetic variation of PvDBPII among P. vivax field isolates from different geographical regions, including Brazil, Colombia, South Korea, Papua New Guinea, Thailand, showed that the PvDBPII is highly polymorphic, but the cysteine residues are conserved within and between P. vivax populations from different geographic regions [14-21]. Although it has been suggested that these polymorphisms do not significantly alter host-parasite binding [17,22], some of them alter immune recognition of PvDBP [23] and most of the PvDBP-specific antibodies detected in infected individuals recognize PvDBPII, rather than other PvDBP regions [7,15].…”

Section: Introductionmentioning

confidence: 99%

“…Although it has been suggested that these polymorphisms do not significantly alter host-parasite binding [17,22], some of them alter immune recognition of PvDBP [23] and most of the PvDBP-specific antibodies detected in infected individuals recognize PvDBPII, rather than other PvDBP regions [7,15]. Consequently, the polymorphic nature of PvDBP, particularly PvDBPII, represents a major impediment to the successful design of a protective vaccine against vivax malaria [14]. Therefore, understanding the nature and genetic polymorphism in PvDBPII among P. vivax isolates from distinct geographic areas, particularly where a large proportion of P. vivax infections occurs, is important for the rational design of vaccines against vivax malaria.…”

Section: Introductionmentioning

confidence: 99%

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Genetic polymorphism and natural selection of Duffy binding protein of Plasmodium vivax Myanmar isolates

Kang

Moon

Kim

et al. 2012

Malar J

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BackgroundPlasmodium vivax Duffy binding protein (PvDBP) plays an essential role in erythrocyte invasion and a potential asexual blood stage vaccine candidate antigen against P. vivax. The polymorphic nature of PvDBP, particularly amino terminal cysteine-rich region (PvDBPII), represents a major impediment to the successful design of a protective vaccine against vivax malaria. In this study, the genetic polymorphism and natural selection at PvDBPII among Myanmar P. vivax isolates were analysed.MethodsFifty-four P. vivax infected blood samples collected from patients in Myanmar were used. The region flanking PvDBPII was amplified by PCR, cloned into Escherichia coli, and sequenced. The polymorphic characters and natural selection of the region were analysed using the DnaSP and MEGA4 programs.ResultsThirty-two point mutations (28 non-synonymous and four synonymous mutations) were identified in PvDBPII among the Myanmar P. vivax isolates. Sequence analyses revealed that 12 different PvDBPII haplotypes were identified in Myanmar P. vivax isolates and that the region has evolved under positive natural selection. High selective pressure preferentially acted on regions identified as B- and T-cell epitopes of PvDBPII. Recombination may also be played a role in the resulting genetic diversity of PvDBPII.ConclusionsPvDBPII of Myanmar P. vivax isolates displays a high level of genetic polymorphism and is under selective pressure. Myanmar P. vivax isolates share distinct types of PvDBPII alleles that are different from those of other geographical areas. These results will be useful for understanding the nature of the P. vivax population in Myanmar and for development of PvDBPII-based vaccine.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%