Diversity and molecular characterization of novel hemoplasmas infecting wild rodents from different Brazilian biomes

Gonçalves, Luiz Ricardo; Roque, André Luiz Rodrigues; Matos, Carlos António; Fernandes, Sérgio Rodrigo; Olmos, Isabella Delamain Fernandez; Machado, Rosangela Zacarias; André, Marcos Rogério

doi:10.1016/j.cimid.2015.10.006

Cited by 23 publications

(38 citation statements)

References 36 publications

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“…The found occurrence of Mycoplasma among wild rodents sampled in the present study was lower (1/110) than that found in a previous recent study involving wild rodents (4/32) in the Pantanal, Brazil (Gonçalves et al, 2015). However, the occurrence of Mycoplasma infection found among wild rodents in Pantanal biome in the above mentioned study was significantly lower than that found in the others three Brazilian biomes (Cerrado, Amazon Forest, and Atlantic Forest) (Gonçalves et al, 2015). Additionally, high occurrence of hemoplasmas was found among capybaras (64%), synanthropic and laboratory rodents (Rattus novergicus) (63.5%) from urban areas in the state of Paraná, Brazil (Vieira et al, 2009;Conrado et al, 2015).…”

Section: Discussioncontrasting

confidence: 88%

“…A closer contact among rodents from urban areas with different host species and arthropods could explain the differences observed in prevalence rates. In spite of that, a high diversity of Mycoplasma genotypes has been detected in rodents from Japan, Hungary and Brazil (Vieira et al, 2009;Sashida et al, 2013;Conrado et al, 2015;Gonçalves et al, 2015;Hornok et al, 2015). A genotype closely related to M. haemomuris was detected in a wild rodent (O. mamorae) in the present study.…”

Section: Discussioncontrasting

confidence: 75%

“…Although hemoplasmas have been detected in domestic cats (Braga et al, 2012;Miceli et al, 2013;André et al, 2014;Santis et al, 2014) and dogs (Ramos et al, 2010;Alves et al, 2014;Valle et al, 2014;Soares et al, 2016) from several localities in Brazil, few reports have documented the occurrence of hemoplasma species in wild animals. For instance, hemoplasmas have been detected in wild carnivores maintained in captivity in zoos (Willi et al, 2007;Guimarães et al, 2007;André et al, 2011), wild rodents (Vieira et al, 2009;Conrado et al, 2015;Gonçalves et al, 2015), monkeys (Bonato et al, 2015), and deer (Grazziotin et al, 2011) in Brazil.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland

et al. 2017

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Hemotropic mycoplasmas are known to cause anemia in several mammalian species. The present work aimed to investigate the occurrence of Mycoplasma spp. in wild mammals, domestic dogs and their respective ectoparasites, in southern Pantanal region, central-western Brazil. Between August 2013 and March 2015, 31 Nasua nasua, 78 Cerdocyon thous, seven Leopardus pardalis, 42 dogs, 110 wild rodents, and 30 marsupials were trapped and ectoparasites (ticks and fleas) found parasitizing the animals were collected. Mammals and ectoparasites DNA samples were submitted to conventional PCR assays for Mycoplasma spp. targeting 16S rRNA and RnaseP genes. Twenty-four N. nasua, three C. thous, two domestic dogs, one L. pardalis and one wild rodent were positive for 16S rRNA PCR protocols. Fourteen N. nasua samples were also positive in RnaseP PCR. No marsupial or arthropod showed positivity for Mycoplasma spp. The phylogenetic analyses based on 16S rRNA gene showed that all sequences obtained from dogs, two sequences obtained from C. thous and ten sequences obtained from N. nasua showed to be closely related to Mycoplasma haemocanis/Mycoplasma haemofelis species. Genotypes closely related to 'Candidatus Mycoplasma haemominutum' and Mycoplasma haemomuris were detected in the L. pardalis and in the wild rodent, respectively. Probably a novel Mycoplasma genotype, closely related to a sequence obtained from a Brazilian capybara was detected in 14 N. nasua, based on a concatenated phylogenetic analysis of 16S rRNA and RnaseP genes. The present study revealed that wild animals in southern Pantanal region, Brazil, are exposed to different species of hemoplasmas.

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Section: Discussioncontrasting

confidence: 88%

Section: Discussioncontrasting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

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Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland

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“…in the present study (54). Consequently, a given genotype may circulate in different rodent species coexisting in a certain biome, as described for hemoplasmas, another group of pathogens also transmitted between rodents by arthropods (55). Additionally, the number of Bartonella spp.…”

Section: Discussionmentioning

confidence: 99%

Association of Bartonella Species with Wild and Synanthropic Rodents in Different Brazilian Biomes

Gonçalves

Favacho

Roque

et al. 2016

Appl Environ Microbiol

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Bartonella spp. comprise an ecologically successful group of microorganisms that infect erythrocytes and have adapted to different hosts, which include a wide range of mammals, besides humans. Rodents are reservoirs of about two-thirds of Bartonella spp. described to date; and some of them have been implicated as causative agents of human diseases. In our study, we performed molecular and phylogenetic analyses of Bartonella spp. infecting wild rodents from five different Brazilian biomes. In order to characterize the genetic diversity of Bartonella spp., we performed a robust analysis based on three target genes, followed by sequencing, Bayesian inference, and maximum likelihood analysis. Bartonella spp. were detected in 25.6% (117/457) of rodent spleen samples analyzed, and this occurrence varied among different biomes. The diversity analysis of gltA sequences showed the presence of 15 different haplotypes. Analysis of the phylogenetic relationship of gltA sequences performed by Bayesian inference and maximum likelihood showed that the Bartonella species detected in rodents from Brazil was closely related to the phylogenetic group A detected in other cricetid rodents from North America, probably constituting only one species. Last, the Bartonella species genogroup identified in the present study formed a monophyletic group that included Bartonella samples from seven different rodent species distributed in three distinct biomes. In conclusion, our study showed that the occurrence of Bartonella bacteria in rodents is much more frequent and widespread than previously recognized. IMPORTANCEIn the present study, we reported the occurrence of Bartonella spp. in some sites in Brazil. The identification and understanding of the distribution of this important group of bacteria may allow the Brazilian authorities to recognize potential regions with the risk of transmission of these pathogens among wild and domestic animals and humans. In addition, our study accessed important gaps in the biology of this group of bacteria in Brazil, such as its low host specificity, high genetic diversity, and relationship with other Bartonella spp. detected in rodents trapped in America. Considering the diversity of newly discovered Bartonella species and the great ecological plasticity of these bacteria, new studies with the aim of revealing the biological aspects unknown until now are needed and must be performed around the world. In this context, the impact of Bartonella spp. associated with rodents in human health should be assessed in future studies.

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“…have been detected in a variety of wild animals all over the world. In Brazil, these agents have been reported in several wild mammals, such as wild carnivores [15][16][17], deer [18], peccary [19], non-human primates [20,21], and rodents [22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Evidence and molecular characterization ofBartonellaspp. and hemoplasmas in neotropical bats in Brazil

et al. 2017

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The order Chiroptera is considered the second largest group of mammals in the world, hosting important zoonotic virus and bacteria. Bartonella and hemotropic mycoplasmas are bacteria that parasite different mammals' species, including humans, causing different clinical manifestations. The present work aimed investigating the occurrence and assessing the phylogenetic positioning of Bartonella spp. and Mycoplasma spp. in neotropical bats sampled from Brazil. Between December 2015 and April 2016, 325 blood and/or tissues samples were collected from 162 bats comprising 19 different species sampled in five states of Brazil. Out of 322 bat samples collected, while 17 (5·28%) were positive to quantitative PCR for Bartonella spp. based on nuoG gene, 45 samples (13·97%) were positive to cPCR assays for hemoplasmas based on 16S rRNA gene. While seven sequences were obtained for Bartonella (nuoG) (n = 3), gltA (n = 2), rpoB (n = 1), ftsZ (n = 1), five 16S rRNA sequences were obtained for hemoplasmas. In the phylogenetic analysis, the Bartonella sequences clustered with Bartonella genotypes detected in bats sampled in Latin America countries. All five hemoplasmas sequences clustered together as a monophyletic group by Maximum Likelihood and Bayesian Inference analyses. The present work showed the first evidence of circulation of Bartonella spp. and hemoplasmas among bats in Brazil.

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Diversity and molecular characterization of novel hemoplasmas infecting wild rodents from different Brazilian biomes

Cited by 23 publications

References 36 publications

Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland

Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland

Association of Bartonella Species with Wild and Synanthropic Rodents in Different Brazilian Biomes

Evidence and molecular characterization ofBartonellaspp. and hemoplasmas in neotropical bats in Brazil

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