Diversity and functional analysis of luxS genes in Vibrios from marine sponges Mycale laxissima and Ircinia strobilina

Zan, Jindong; Fuqua, Clay; Hill, Russell T.

doi:10.1038/ismej.2011.31

Cited by 24 publications

(21 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This diversity of luxS genes in our study agree with previous studies (Gattiker et al, 2002;Zan et al, 2011) which reported that, LuxS genes are greatly divergent among Gram-positive and Gram-negative bacteria, but may have the same functions and are considered to be conserved in many bacterial species.…”

Section: Discussionsupporting

confidence: 93%

Quorum sensing signal production by sponge-associated bacteria isolated from the Red Sea, Egypt

Yahia¹,

Hanora²,

Mahmoud³

et al. 2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Bacteria regulate their social activities and physiological processes through a quorum sensing (QS) mechanism. Many bacteria inhabit the mesohyl matrix of sponges. This symbiotic relationship is believed to contribute to the health and nutrition of sponges by production of protective antibiotics, the acquisition of limiting nutrients and nitrogen fixation. Marine isolates were analysed by bioassays using two different acylated homoserine lactone (AHL) reporter bacteria Chromobacterium violaceum 026 (CV026) and Agrobacterium tumefaciens KYC55. Thirty four marine isolates activated the A. tumefaciens and produce blue color. Using degenerate LuxS primers, partial LuxS gene sequences were detected in both Gram-negative and Gram-positive species including Bacillus species, Proteus species, uncultured Bacterium species, Micrococcus species and Bacterium NLAE-zl-H221. Using degenerate LuxR primers, partial LuxR gene sequences were detected in Proteus spp., Providencia species and Bacterium NLAE-zl-H221. Partial LuxS gene sequences were detected in both Gramnegative and Gram-positive. The results thus assure the fact that LuxS gene has been proposed to be a universal interspecies signaling molecule. This study showed that, Proteus mirabilis strain SZH18, Proteus vulgaris and Bacterium NLAE-zl-H221 have both luxS and luxR genes, thus it was claimed that these isolates have the potential to use two different QS systems, for intraspecies communication and communication with other species.

show abstract

Section: Discussionsupporting

confidence: 93%

Quorum sensing signal production by sponge-associated bacteria isolated from the Red Sea, Egypt

Yahia¹,

Hanora²,

Mahmoud³

et al. 2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Samples of M. laxissima were collected by SCUBA diving at Conch Reef, Key Largo, Florida in late October 2008 at a depth of c. 20 m as described by Zan et al . (2011a).…”

Section: Methodsmentioning

confidence: 99%

“…Samples of M. laxissima were collected by SCUBA diving at Conch Reef, Key Largo, Florida in late October 2008 at a depth of c. 20 m as described by Zan et al (2011a). Sponge samples were rinsed 3¥ with sterile artificial seawater (ASW) and were stored in RNAlater solution (Qiagen, Valencia, CA, USA) immediately for RNA extraction.…”

Section: Rna Extraction and Rt-pcr From Sponge Tissuementioning

confidence: 99%

“…Although certain bacteria serve as nutrients, there is evidence for stable symbionts, including several that are vertically transmitted (Enticknap et al ., 2006; Sharp et al ., 2007; Schmitt et al ., 2008). Within the densely colonized sponge, there is also ample opportunity for both inter‐species and intra‐species signalling (Zan et al ., 2011a).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A complex LuxR–LuxI type quorum sensing network in a roseobacterial marine sponge symbiont activates flagellar motility and inhibits biofilm formation

Zan

Cicirelli

Mohamed

et al. 2012

Molecular Microbiology

Self Cite

View full text Add to dashboard Cite

Summary Bacteria isolated from marine sponges, including the Silicibacter–Ruegeria (SR) subgroup of the Roseobacter clade, produce N‐acylhomoserine lactone (AHL) quorum sensing signal molecules. This study is the first detailed analysis of AHL quorum sensing in sponge‐associated bacteria, specifically Ruegeria sp. KLH11, from the sponge Mycale laxissima. Two pairs of luxR and luxI homologues and one solo luxI homologue were identified and designated ssaRI, ssbRI and sscI (sponge‐associated symbiont locus A, B and C, luxR or luxI homologue). SsaI produced predominantly long‐chain 3‐oxo‐AHLs and both SsbI and SscI specified 3‐OH‐AHLs. Addition of exogenous AHLs to KLH11 increased the expression of ssaI but not ssaR, ssbI or ssbR, and genetic analyses revealed a complex interconnected arrangement between SsaRI and SsbRI systems. Interestingly, flagellar motility was abolished in the ssaI and ssaR mutants, with the flagellar biosynthesis genes under strict SsaRI control, and active motility only at high culture density. Conversely, ssaI and ssaR mutants formed more robust biofilms than wild‐type KLH11. AHLs and the ssaI transcript were detected in M. laxissima extracts, suggesting that AHL signalling contributes to the decision between motility and sessility and that it may also facilitate acclimation to different environments that include the sponge host.

show abstract

“…This dense microbial community associated with sponges provides conditions in which quorum sensing (QS) may be important (3,8). QS is a process by which bacteria coordinate group activities such as bioluminescence, antibiotic production, and virulence at high cell density via signal molecules, typified by N-acylhomoserine lactone (AHL) in Proteobacteria (2).…”

mentioning

confidence: 99%

Genome Sequence of Ruegeria sp. Strain KLH11, anN-Acylhomoserine Lactone-Producing Bacterium Isolated from the Marine Sponge Mycale laxissima

et al. 2011

Self Cite

View full text Add to dashboard Cite

Ruegeria sp. strain KLH11, isolated from the marine sponge Mycale laxissima, produces a complex profile of N-acylhomoserine lactone quorum-sensing (QS) molecules. The genome sequence provides insights into the genetic potential of KLH11 to maintain complex QS systems, and this is the first genome report of a cultivated symbiont from a marine sponge.Sponges can harbor diverse assemblages of microbes, which in some cases can comprise up to 60% of the total biomass of the holobiont (6). This dense microbial community associated with sponges provides conditions in which quorum sensing (QS) may be important (3,8). QS is a process by which bacteria coordinate group activities such as bioluminescence, antibiotic production, and virulence at high cell density via signal molecules, typified by N-acylhomoserine lactone (AHL) in Proteobacteria (2). We isolated a Ruegeria sp. strain, KLH11 [99% 16S rRNA gene sequence similarity with Ruegeria lacuscaerulensis (GenBank accession no. HQ908678)], which can produce a complex profile of long-chain AHLs (3). The Silicibacter-Ruegeria subgroup belongs to the Roseobacter clade, which is widespread and numerous in surface waters of the oceans and includes organisms that are involved in global sulfur cycling (1). Many roseobacters have been fully sequenced and annotated (5). Ruegeria sp. strain KLH11 is the first readily culturable sponge symbiont to have its genome sequenced. This microorganism has been isolated from several samples of the sponge Mycale laxissima and is termed a symbiont using the term as defined by Taylor et al. (6).The genome was sequenced by a combination of Roche/454 and Sanger whole-genome shotgun sequencing. Sequences were assembled with a Celera assembler (4), and genes were predicted and annotated using the automated CloVR-Microbe pipeline (7).The draft genome has a total assembly size of ca. 4.5 Mb, with an average GϩC content of 57.3 mol%, and is predicted to have 4,493 coding sequences (CDS), of which 3,243 (ca. 72.0%) were functionally annotated. This assembly is comprised of 13 scaffolds, of which the largest is ca. 3.1 Mb, having 3,193 CDS. One scaffold of 81 kbp shows similarity to the megaplasmid identified in Ruegeria pomeroyi DSS-3 (accession number CP000032) and could indicate the presence of extrachromosomal elements in the genome. Approximately 82.0% of all the predicted CDS have ATG sequences at the presumptive start position, and 17.8% may use GTG or TTG. One complete rRNA operon, one partial 16S rRNA gene, two 5S rRNA genes, and 52 tRNAs were identified. About 34 genes were found to be related to the synthesis of bacterial flagella and regulation of bacterial motility. No candidates for chemotaxis functions were observed in the genome. Two sets of quorum-sensing circuits were identified, implying the potential for a complex quorum-sensing mechanism. Roughly 44 genes were predicted to function in the biosynthesis and degradation of polysaccharides.Comparison of KLH11 genome sequences to closely related free-living bacteria, such as R. pomeroyi ...

show abstract

Diversity and functional analysis of luxS genes in Vibrios from marine sponges Mycale laxissima and Ircinia strobilina

Cited by 24 publications

References 51 publications

Quorum sensing signal production by sponge-associated bacteria isolated from the Red Sea, Egypt

Quorum sensing signal production by sponge-associated bacteria isolated from the Red Sea, Egypt

A complex LuxR–LuxI type quorum sensing network in a roseobacterial marine sponge symbiont activates flagellar motility and inhibits biofilm formation

Genome Sequence of Ruegeria sp. Strain KLH11, anN-Acylhomoserine Lactone-Producing Bacterium Isolated from the Marine Sponge Mycale laxissima

Contact Info

Product

Resources

About