Diversity and EPS Production Potential of Halotolerant Bacteria from Veraval and Dwarka

Nanjani, Sandhya

doi:10.9790/3008-0222025

Cited by 7 publications

(5 citation statements)

References 24 publications

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“…Moderately tolerant, up to 18–20% (w/v). Extremely tolerant, those microbes that grow over the whole range of salt concentrations from zero up to saturation 3 .…”

Section: Introductionmentioning

confidence: 99%

Plant growth promoting characteristics of halophilic and halotolerant bacteria isolated from coastal regions of Saurashtra Gujarat

Reang

Bhatt

Tomar

et al. 2022

Sci Rep

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Halophiles are a class of microorganisms that thrive in environments with very high salt concentrations. The coastal regions of Saurashtra Gujarat host a diverse group of microorganisms including halophilic and halotolerant bacteria that may have plant growth promoting characteristics. Microorganisms with plant growth promoting characteristics are of immense importance in the field of agriculture and the present study was conducted to investigate the characteristics of halophilic and halotolerant bacteria isolated from agricultural soils of coastal regions of Junagadh and Porbandar districts of Saurashtra, Gujarat. A total of 15 isolated strains showed indole acetic acid production, solubilization of phosphate and potash, and nitrogen fixing capacity ranging from 18.77–33.48 μg ml−1, 50.10–106.10%, 180.42–239.92% and 0.170–0.480 g kg−1 of Jensen’s agar medium, respectively, while two isolates were also found positive for siderophore production. Besides, nine out of fifteen isolates also showed positive ACC deaminase activity ranging from 0.92-5.78 µM α-ketobutyrate mg−1 h−1. The isolates were further characterized by physiological, microscopic, and biochemical tests. The halophilic and halotolerant bacterial isolates were identified by 16S rRNA gene sequencing as belonging to Halomonas pacifica, H. stenophila, and Bacillus haynesii, B. licheniformis and Oceanobacillus aidingensis respectively. The 16S rRNA partial gene sequence of two isolates belonging to H. pacifica and H. stenophila were submitted to NCBI with accession number MK955347 and MK961217 respectively. The findings of the present investigation showed that isolated bacterial halophiles possess promising plant growth promoting characteristics. Their potential as bioinoculants to alleviate salinity stress in crops and for bioremediation deserves further investigation.

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“…Moderately tolerant, up to 18–20% (w/v). Extremely tolerant, those microbes that grow over the whole range of salt concentrations from zero up to saturation 3 .…”

Section: Introductionmentioning

confidence: 99%

Plant growth promoting characteristics of halophilic and halotolerant bacteria isolated from coastal regions of Saurashtra Gujarat

Reang

Bhatt

Tomar

et al. 2022

Sci Rep

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“…The 80 strains with emulsifications (EI 24 ) ≥50% and/or with a surface tension by the drop-collapse ≥ 4 mm, were evaluated at concentrations of 1% to 20% NaCl ( Table S1 ), considering halotolerant those that grow at concentrations above 1% of salt [ 36 ]; therefore 94% are halotolerant and only 4% do not tolerate salt. Most active strains grow between 2.5% and 20%, classifying them as moderately halotolerant, more frequent than those growing at 10% NaCl, while 21% border on extreme halotolerant [ 19 , 37 ].…”

Section: Resultsmentioning

confidence: 99%

“…The 80 strains with emulsifications (EI24) ≥50% and/or with a surface tension by the drop-collapse ≥4 mm, were evaluated at concentrations of 1% to 20% NaCl (Table S1), considering halotolerant those that grow at concentrations above 1% of salt [36]; therefore BE activity was observed with a similar percentage of bacteria in both sinkholes, TZ with 85% and XH with 83% (Figure 1); of these, 33.5% produce EI 24 ≥ 50%. This suggests that the cultivable microorganisms from these sites can produce metabolites with BE activity, which may be a mechanism to efficiently solubilize low nutrients in this oligotrophic environment [18].…”

Section: Stability Of the Bs And Be Activity Under Salinity Conditionsmentioning

confidence: 99%

Bacteria Halotolerant from Karst Sinkholes as a Source of Biosurfactants and Bioemulsifiers

et al. 2022

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Halotolerant bacteria with biosurfactant (BS) and bioemulsifiers (BE) activity can coexist in Karstic sinkholes with marine influence. Two sinkholes in the Yucatan peninsula were selected to isolate bacteria with BE and BS activity stable in NaCl. The optimal time, the effect of nitrogen and carbon source in the medium, and the conditions (agitation, pH and salinity) for the production of BS and BE compounds in planktonic and sessile (stimulate the formation of biofilms in cell roller) culture were determined. Eighty strains showed the highest emulsification activity (EI24 ≥ 50%) and drop-collapse ≥ 4 mm. 87% of the strains are moderately halotolerant, and 21% bordered the limit of extreme halotolerance. Twenty-four strains maintained or improved their BS and BE activity under salinity conditions at 5% and 10%, being the most active genera Bacillus, Paenibacillus and Lysinibacillus, identified by sequencing of the 16S rRNA gene. The results show that the nitrogen source positively affects the BS and BE activity, regardless of the type of culture. The sessile culture markedly stimulated BS activity with significant differences. However, we did not find a greater influence on the culture conditions. The results suggest that halotolerant bacteria from sinkholes could be implemented in bioremediation and other biotechnological applications.

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“…There are some reports on the diversity study of marine salterns near Bhavnagar and from the coastal region of Dwarka-Veraval. According to that the microbial diversity and growth of the microorganisms found to decrease with higher salt concentration (Dave and Desai, 2006;Nanjani and Soni, 2012). Gram positive bacteria were more in numbers between 10-15% of NaCl concentrations; whereas, gram negative isolates showed dominance between 20-25% of NaCl concentrations.…”

Section: Figure 1amentioning

confidence: 99%

Diversity Assessment and Eps Production Potential of Cultivable Bacteria From the Samples of Coastal Site of Alang

Upadhyay¹,

Vaishnav²,

Tipre³

et al. 2016

J microb biotech food sci

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In this study diversity of cultivable bacteria and their exopolysaccharide production potential were investigated from the water and sediment samples of coastal site of ALang. The studied ten samples represented diversity in pH, conductivity, salinity and TDS, in the range of 7.25 to 8.92, 9.4 to 76.8 mS, 3570 to 35100 mg/L and 15120 to 53100 mg/L respectively. Total 141 bacteria were isolated from the collected ten samples, from which 105 were gram positive and 36 were gram negative in nature. Amongst the isolates 33% were able to produce variety of pigments. The diversity indices, including Shannon-Wiener index (H’), Richness, and Evenness Indices based on the metabolic characteristics of the organisms were calculated. The isolates were characterized morphologically and biochemically. Identification of 41 isolates was confirmed by 16S rRNA gene sequencing. They represented 22 genera and various species of these genera. The bacterial isolates were able to grow in the range of 3-25% NaCl concentrations. When 141 cultures were grown in liquid medium, the viscosity of the medium ranged between 9.20x10-6 to 2.09x10-4 m.pa.s-1. The EPS yield in terms of dry weight also ranged from 0.76 to 10.7g/L.

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Diversity and EPS Production Potential of Halotolerant Bacteria from Veraval and Dwarka

Abstract: The microbial EPS are used in several biotechnological applications viz., cosmetics, textiles, pharmaceuticals, agricultural, paints

Cited by 7 publications

References 24 publications

Plant growth promoting characteristics of halophilic and halotolerant bacteria isolated from coastal regions of Saurashtra Gujarat

Plant growth promoting characteristics of halophilic and halotolerant bacteria isolated from coastal regions of Saurashtra Gujarat

Bacteria Halotolerant from Karst Sinkholes as a Source of Biosurfactants and Bioemulsifiers

Diversity Assessment and Eps Production Potential of Cultivable Bacteria From the Samples of Coastal Site of Alang

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