Diversity and ecology of fungal assemblages present in lake sediments at Clearwater Mesa, James Ross Island, Antarctica, assessed using metabarcoding of environmental DNA

Gonçalves, Vívian N.; Souza, Láuren Machado Drumond de; Lirio, Juan Manuel; Coria, Silvia H.; Lopes, Fabyano Álvares Cardoso; Convey, Peter; Carvalho‐Silva, Micheline; Oliveira, Fábio Soares de; Câmara, Paulo Eduardo Aguiar Saraiva; Rosa, Luiz Henrique

doi:10.1016/j.funbio.2022.08.002

Cited by 11 publications

(10 citation statements)

References 51 publications

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“…Comparable results were obtained by Perini et al [30], who calculated fungal diversity in waters of a lake from Ny-Ålesund with a Shannon index H = 3.27, corresponding to the mean value obtained in this study (H = 3.3), while greater values (comprised between H = 3.83 and 5.24) were obtained by Zhang et al [2]. The diversity data of fungal sequence assemblages detected in the sediment of Antarctic lakes LA, LB, LS, LT, and LZ studied here were greater than those reported in previous culture-based studies [1,10,24], and comparable with those reported in DNA metabarcode study by Ogaki et al [25], de Souza et al [26], Rosa et al [27], and Gonçalves et al [37] for other Antarctic lakes. However, results obtained by de Souza et al [26] in sediment of Soto Lake, located in Deception Island (Antarctic Peninsula), which hosts three of the lakes examined in the present study (i.e., Balleneros, Telefon and Zapatilla), showed lower diversity indices (Fischer = 10.27).…”

Section: Fungal Diversitysupporting

confidence: 87%

“…The most represented identified phyla were, in order, Chytridiomycota, Rozellomycota, Ascomycota, Basidiomycota, which were commonly reported by Comeau et al [29], Zhang et al [2] and Perini et al [30] in Arctic lakes. Less frequent, instead, were Monoblepharomycota, Aphelidiomycota, Mortierellomycota, Neocallimastigomycota and Mucoromycota which were never reported in Arctic lakes, but (excluding the phylum Neocallimastigomycota) were previously detected in Antarctic lakes [25][26][27]37].…”

Section: Fungal Phylamentioning

confidence: 88%

“…The genus Betamyces (Chytridiomycota) was retrieved in freshwater ecosystem in Ny-Ålesund (Arctic) [2] and in sediment of Antarctic lakes, where Betamyces spp. dominated the assemblages [37]. The genus includes only one known species, Betamyces americae-meridionalis, which was isolated from pollen baits at the Paraná River (Buenos Aires, Argentina) and in soil in Costa Rica [51].…”

Section: Fungal Generamentioning

confidence: 99%

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A Deep Insight into the Diversity of Microfungal Communities in Arctic and Antarctic Lakes

Marchetta,

Papale,

Rappazzo

et al. 2023

JoF

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We assessed fungal diversity in water and sediment samples obtained from five Arctic lakes in Ny-Ålesund (Svalbard Islands, High Arctic) and five Antarctic lakes on Livingston and Deception Islands (South Shetland Islands), using DNA metabarcoding. A total of 1,639,074 fungal DNA reads were detected and assigned to 5980 ASVs amplicon sequence variants (ASVs), with only 102 (1.7%) that were shared between the two Polar regions. For Arctic lakes, unknown fungal taxa dominated the sequence assemblages, suggesting the dominance of possibly undescribed fungi. The phylum Chytridiomycota was the most represented in the majority of Arctic and Antarctic samples, followed by Rozellomycota, Ascomycota, Basidiomycota, and the less frequent Monoblepharomycota, Aphelidiomycota, Mortierellomycota, Mucoromycota, and Neocallimastigomycota. At the genus level, the most abundant genera included psychrotolerant and cosmopolitan cold-adapted fungi including Alternaria, Cladosporium, Cadophora, Ulvella (Ascomycota), Leucosporidium, Vishniacozyma (Basidiomycota), and Betamyces (Chytridiomycota). The assemblages displayed high diversity and richness. The assigned diversity was composed mainly of taxa recognized as saprophytic fungi, followed by pathogenic and symbiotic fungi.

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Section: Fungal Diversitysupporting

confidence: 87%

Section: Fungal Phylamentioning

confidence: 88%

Section: Fungal Generamentioning

confidence: 99%

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A Deep Insight into the Diversity of Microfungal Communities in Arctic and Antarctic Lakes

Marchetta,

Papale,

Rappazzo

et al. 2023

JoF

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“…Ciliophora is a genus of Ascomycota reported to be saprophytic and recovered from tropical environments of Central America 42 , with currently only two known species, C. cryptica and C. quercus ( www.mycobank.org ). In Antarctica, sequences assigned to Ciliophora have been detected with low and medium relative abundance in DNA metabarcoding studies of lake sediments 43 , 44 and soils 45 from James Ross Island, and in wood samples from historic anthropogenic structures on Deception Island 46 . The fungal-like Straminopila genus Chaetoceros was described by C. Ehrenberg in 1844 based on Antarctic material containing the type species C. dichaeta , and also occurs in other oceans including in the Arctic 47 .…”

Section: Discussionmentioning

confidence: 99%

Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding

Silva

Souza

Vieira

et al. 2022

Sci Rep

Self Cite

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We assessed the fungal and fungal-like sequence diversity present in marine sediments obtained in the vicinity of the South Shetland Islands (Southern Ocean) using DNA metabarcoding through high-throughput sequencing (HTS). A total of 193,436 DNA reads were detected in sediment obtained from three locations: Walker Bay (Livingston Island) at 52 m depth (48,112 reads), Whalers Bay (Deception Island) at 151 m (104,704) and English Strait at 404 m (40,620). The DNA sequence reads were assigned to 133 distinct fungal amplicon sequence variants (ASVs) representing the phyla Ascomycota, Basidiomycota, Mortierellomycota, Chytridiomycota, Glomeromycota, Monoblepharomycota, Mucoromycota and Rozellomycota and the fungal-like Straminopila. Thelebolus balaustiformis, Pseudogymnoascus sp., Fungi sp. 1, Ciliophora sp., Agaricomycetes sp. and Chaetoceros sp. were the dominant assigned taxa. Thirty-eight fungal ASVs could only be assigned to higher taxonomic levels, and may represent taxa not currently included in the available databases or represent new taxa and/or new records for Antarctica. The total fungal community displayed high indices of diversity, richness and moderate to low dominance. However, diversity and taxa distribution varied across the three sampling sites. In Walker Bay, unidentified fungi were dominant in the sequence assemblage. Whalers Bay sediment was dominated by Antarctic endemic and cold-adapted taxa. Sediment from English Strait was dominated by Ciliophora sp. and Chaetoceros sp. These fungal assemblages were dominated by saprotrophic, plant and animal pathogenic and symbiotic taxa. The detection of an apparently rich and diverse fungal community in these marine sediments reinforces the need for further studies to characterize their richness, functional ecology and potential biotechnological applications.

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“…The use of recent high‐throughput next‐generation sequencing techniques in metabarcoding, which enables an efficient large‐scale analysis of DNA samples (Taberlet et al., 2012), has led to a better understanding of fungal diversity (Picard, 2017; Polinski et al., 2019; Rämä et al., 2016). Environmental metabarcoding thus offers a powerful technique to analyze still unexplored benthic habitats (da Silva et al., 2022; Gonçalves et al., 2022; Li et al., 2016; Polinski et al., 2019; Wu et al., 2023). These techniques have rarely been employed in the study of coastal benthic fungal diversity, particularly in the brackish Baltic Sea and at large spatial scales (i.e.…”

Section: Introductionmentioning

confidence: 99%

Salinity and resource availability as drivers of Baltic benthic fungal diversity

Lobo,

Izabel‐Shen,

Albertsson

et al. 2024

Environmental DNA

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Marine biodiversity consists of a complex network of organisms responsible for keeping the ecosystem's balance. Fungi are an understudied group of organisms despite their recognized importance for ecosystem processes and diversity. How fungi respond to environmental change remains poorly understood, especially in marine benthic habitats. The Baltic Sea is a brackish coastal ecosystem with steep environmental gradients in a relatively limited geographical area and is therefore a particularly good system to investigate the impact of different abiotic factors on benthic fungal diversity. This study used environmental DNA (eDNA) metabarcoding to analyze the spatial dynamics of benthic fungal diversity in the Baltic Sea and quantify the environmental drivers that shape these dynamics. Based on 59 stations spreading over 1145 km the results showed that benthic fungal communities were dominated by the phylum Chytridiomycota, and the fungal species Alphamyces chaetifer and Operculomyces laminatus from this phylum were the main drivers of the community structure dissimilarities observed between regions. Water depth and salinity were the main predictors of the benthic fungal community composition. The impact of nutrient availability was also significant, possibly related to the known role of Chytridiomycota species such as A. chaetifer and O. laminatus in nutrient cycling. Our results indicate that the benthic fungal diversity of the Baltic Sea is shaped by salinity gradients and nutrient availability and highlights that the current fungal biodiversity is at risk of species shift or decline with predicted changes in salinity due to climate change and intensified eutrophication.

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Diversity and ecology of fungal assemblages present in lake sediments at Clearwater Mesa, James Ross Island, Antarctica, assessed using metabarcoding of environmental DNA

Cited by 11 publications

References 51 publications

A Deep Insight into the Diversity of Microfungal Communities in Arctic and Antarctic Lakes

A Deep Insight into the Diversity of Microfungal Communities in Arctic and Antarctic Lakes

Fungal and fungal-like diversity in marine sediments from the maritime Antarctic assessed using DNA metabarcoding

Salinity and resource availability as drivers of Baltic benthic fungal diversity

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