Diversity and ecology of culturable marine fungi associated with Posidonia oceanica leaves and their epiphytic algae Dictyota dichotoma and Sphaerococcus coronopifolius

Pasqualetti, Marcella; Giovannini, Valeria; Barghini, Paolo; Gorrasi, Susanna; Fenice, Massimiliano

doi:10.1016/j.funeco.2019.100906

Cited by 18 publications

(25 citation statements)

References 55 publications

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“…The phylogram clearly grouped IG 100 with two different strains of M. humicola with a sequence similarity of 98%. The phylogenetic analysis con rmed previous taxonomical identi cation [30]. In addition, a certain degree of β-tubulin gene differentiation in the marine strain IG100 was observed with respect to other species of M. humicola, namely CBS 102628 (KM232013, from decayed wood), and CBS 740.95 (KM232012, from soil).…”

Section: Resultssupporting

confidence: 81%

“…Phylogenetic Analysis of the fungus The strain IG100 is well adapted to the sea conditions and its growth preferences permitted to considered this fungus as a marine strain of M. humicola. Actually, the strain is a facultative halophile (range of salinity tolerance 0-152% NaCl -optimum at 20%) with a sub-mesophilic behaviour, being able to grow in the range of 10-35 °C, with optimum at 25°C [30]. The taxonomical identi cation was based on the ITS (GenBank accession number: MG976446) and β-tubulin gene (GenBank accession number: MH001469) markers [30].…”

Section: Resultsmentioning

confidence: 99%

“…Actually, the strain is a facultative halophile (range of salinity tolerance 0-152% NaCl -optimum at 20%) with a sub-mesophilic behaviour, being able to grow in the range of 10-35 °C, with optimum at 25°C [30]. The taxonomical identi cation was based on the ITS (GenBank accession number: MG976446) and β-tubulin gene (GenBank accession number: MH001469) markers [30]. In this study a phylogenetic analysis based on the β-tubulin gene was carried out within the Mariannaea genus using the GenBank database, which already include the IG100 strain ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…In particular, fungi related to leaf district are characterized by a high content of tannic acids and represent an interesting source of new bioactive molecules [29]. A great heterogeneity of fungal colonisers from different posidonia meadows in Tyrrhenian Sea was reported [30][31][32], and their biotechnological application is still increasing [20,29]. Mariannaea humicola, belonging to Ascomycetes (Hypocreales, Nectriaceae), is generally recognised as a soil saprophytic fungus (ethimology: name refers to the soil substrate from which this fungus was isolated), [33].…”

Section: Introductionmentioning

confidence: 99%

“…Mariannaea humicola, belonging to Ascomycetes (Hypocreales, Nectriaceae), is generally recognised as a soil saprophytic fungus (ethimology: name refers to the soil substrate from which this fungus was isolated), [33]. Recently, the rst marine strain of the species (M. humicola IG100) was isolated within an ecological study on the marine microbial biodiversity in a P. oceanica meadow [30]. Here we report the puri cation and isolation of two secondary metabolites of the terpenoid family from M. humicola IG100, characterized by a signi cant antifungal activity against Penicillium griseofulvum and Aspergillus avus.…”

Section: Introductionmentioning

confidence: 99%

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Production and identification of two antifungal terpenoids from the Posidonia oceanica epiphytic Ascomycota Mariannaea humicola IG100

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Background Marine fungi are an important repository of bioactive molecules with great potential in different technological fields, the annual number of new compounds isolated from marine fungi is impressive and the general trend indicates that it is still on the rise. In this context, the antifungal and antimicrobial activity of the marine strain Mariannaea humicola IG100 was evaluated and two active terpenoids were isolated and characterized. Methods Preliminary screening of activity of marine strain IG100 was carried out by agar plug diffusion methods against fungal (P. griseofulvum TSF04) and bacterial (Bacillus pumilus KB66 and Escherichia coli JM109) strains. Subsequently, inhibition tests were done by using the cultural broth and the organic extract (ethyl acetate, EtOAc) by the agar well diffusion methods. The main active fractions were identified and tested for their antifungal activity against P. griseofulvum TSF04 in a 24 wells microplate at different concentrations (1000, 100, 10 and 1.0 µg/mL). Two active compounds were characterized and their relative MIC measured by the broth micro-dilution methods in a 96-well microplate against A. flavus IG133, P. griseofulvum TSF04, and T. pleuroticola IG137. Results Marine strain IG100 presented significant antifungal activity associated to two active compounds, the terpenoids terperstacin 1 and 19-acetyl-4-hydroxydictyodiol 2. Their MIC values were measured for Aspergillus flavus (MIC of 7.9 µg/mL and 31.3 µg/mL for 1 and 2, respectively), P. griseofulvum (MIC of 25 µg/mL and 100 µg/mL for 1 and 2, respectively) and T. pleuroticola (MIC > 500 µg/mL and 125 µg/mL for 1 and 2, respectively). They showed a rather good fungistatic effect. Conclusions In this study, the first marine strain of M. humicola (IG100) was investigated for the production of bioactive molecules. Strain IG100 produced significant amounts of two bioactive terpenoids, terperstacin 1 and 19-acetyl-4-hydroxydictyodiol 2. The two compounds showed significant antifungal activities against A. flavus IG133, T. pleuroticola IG137 and P. griseofulvum TSF04. Compound 2 was identified for the first time in fungi.

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Section: Resultssupporting

confidence: 81%

Section: Resultsmentioning

confidence: 99%