Diversity and ecological tolerance of bacteria isolated from the rhizosphere of halophyton plants living nearby Kiskunság soda ponds, Hungary

Abstract

Many halophytes and halophilic microorganisms are capable to adapt to the extremities of saline habitats. This study reveals the taxonomic diversity and ecological tolerance of bacteria isolated from the rhizosphere of three different halophytes (Bolboschoenus maritimus, Puccinellia limosa and Aster tripolium) living in the vicinity of Kiskunság soda ponds. Following a sampling in September 2013, altogether 76 bacterial strains were isolated using two different media. The strains were identifi ed on the basis of 16S rRNA gene sequencing following ARDRA grouping. Salt and pH tolerance of the strains were examined by measuring their growth in broths containing 0-15% NaCl (w/V) and characterized with pH 7-12 values. Among the strains genera of Anaerobacillus, Bacillus and Exiguobacterium (Firmicutes), Agromyces, Isoptericola, Microbacterium, Micrococcus, Nocardiopsis, Nesterenkonia and Streptomyces (Actinobacteria), Halomonas and Idiomarina (Proteobacteria) and Anditalea (Bacteroidetes) were identifi ed. The Bolboschoenus and Puccinellia samples characterized with the highest pH and electric conductivity values were dominated by Bacillus, Halomonas and Nesterenkonia, respectively. The salt tolerance of the bacterial strains was strongly dependent on the sampling location and plant species. In contrast, growth of bacterial strains in broths with alkaline pH values was more balanced. The strains from the Puccinellia sample showed the widest salt and pH tolerance.

Rhizobacterial communities of five co-occurring desert halophytes

“…While community composition also seemed to vary, differences were not significant (Table 2). Halomonas was the dominant genera in Halocnemum strobilaceum communities (32.4% of total abundance), a significantly higher proportion than in the other plant-associated communities; this trend is consistent with previous studies (Al-Mailem et al, 2010; Marasco et al, 2016), including of other halophytes (Borsodi et al, 2015). In the Halostachys caspica rhizosphere, Exiguobacterium was the most common genus, followed by Citrobacter , Acinetobacter and Pseudomonas.…”

“…These studies have revealed that bacterial communities in the halophyte rhizosphere are distinctly different from those of non-halophytic plants, containing a larger proportion of halophilic bacteria (Al-Mailem et al, 2010). Many halophilic bacteria have been identified or isolated from halophyte roots, soils and desert habitats, including species belonging to the following genera: Alkalimonas , Bacillus , Brachybacterium , Brevibacterium, Cronobacter , Halobacillus , Halomonas , Marinococcus , Methylibium , Nesterenkonia , Oceanobacillus , Staphylococcus , Stenotrophomonas , Virgibacillus and Zhihengliuella (Sgroy et al, 2009; Siddikee et al, 2010; Tang et al, 2011; Shi et al, 2012; Zhou et al, 2012; Ramadoss et al, 2013; Borsodi et al, 2015; Zhao et al, 2016). …”

“…In contrast, Acidimicrobiales, Myxococcales and Sphingomonadales are common in the rhizosphere of Halimione portulacoides and Sarcocornia perennis (Oliveira et al, 2014). The Puccinellia limosa rhizosphere is dominated by Halomonas and Nesterenkonia species (Borsodi et al, 2015). Similar rhizobacterial communities may be found in different environments when the same plant species is present (Smalla et al, 2001; Berg & Smalla, 2009).…”

A mikrorespirációs (MicroRespTM) módszer alkalmazása apajpusztai szikes talajok mikrobaközösségeinek katabolikus aktivitás mintázatának vizsgálatára

“…A Zab-szék, Kelemen-szék és Böddi-szék körüli növényzet rhizoszférájából származó baktériumtörzsek só-és pH-toleranciájával foglalkozó eredményeket korábban BORSODI et al (2015) közölték. BÁRÁNY et al (2014 ugyanezen három területről származó néhány növényfaj rhizoszférájából származó minták mikrobaközösségeinek genetikai és katabolikus ujjlenyomatát hasonlították össze, utóbbit a fent említett MicroResp módszerrel.…”

Mikrobiális anyagcsere aktivitás-mintázat és mikorrhiza gomba kolonizáció elemzése három szikes tó melletti talaj rizoszférában

“…Ráadásul az ingadozó hőmérséklet és vízjárás, a kiszáradástól az időszakos vízborításokig tovább fokozzák a környezeti szélsőségeket. Vizsgálatainkat 2013-ban kezdtük a Kiskunsági Nemzeti Park szikes területein, amelynek első eredményeit már közöltük (BÁRÁNY et al 2014;BORSODI et al 2015).…”