The present study summarises spider records from East Macedonia and Thrace (NE Greece). Spiders were mainly collected by hand sampling and to a smaller extent by pitfall trapping during field trips in 2002, 2004, 2007, 2008 and 2009. 171 species from 23 families were sampled. Pelecopsis pavida (O. P.-Cambridge, 1872) (Linyphiidae) and Xysticus kaznakovi Utochkin, 1968 (Thomisidae) were new to the European spider fauna and ten further species, Diaea livens Simon, 1876, Heriaeus graminicola (Doleschall, 1852), Meioneta ressli Wunderlich, 1973, Oedothorax retusus (Westring, 1851), Philodromus albidus Kulczynski, 1911, Pocadicnemis pumila Keyserling, 1880, Sitticus saltator (O. P.-Cambridge, 1868), Stemonyphantes lineatus (Linnaeus, 1758), Synageles scutiger Prószynski, 1979 and Thanatus coloradensis Keyserling, 1880, could be added to the Greek fauna for the first time. 21 and 38 species were new to East Macedonia and Thrace, respectively. Based on previous work, plus the present study, the Greek spider fauna now includes 1,108 species