Diversity and Distribution Characteristics of Viruses from Soda Lakes

Elnosary, Mohamed E.; Ahmed, Marwa M.; Hassanein, Wael S.; Elshafey, Naglaa; Sofy, Ahmed R.; Hamedo, Hend

doi:10.3390/genes14020323

Cited by 9 publications

(10 citation statements)

References 45 publications

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“…According to recent statistics, viruses are thought to be responsible for 2,000,000 human fatalities annually throughout the world [ 25 ]. SARS-CoV), MERS-CoV, and SARS-CoV-2 have all occurred in the world [ 26 , 27 ]. The highly pathogenic MERS-CoV was initially discovered in the KSA in 2012 [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

Identifying the Anti-MERS-CoV and Anti-HcoV-229E Potential Drugs from the Ginkgo biloba Leaves Extract and Its Eco-Friendly Synthesis of Silver Nanoparticles

et al. 2023

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The present study aimed to estimate the antiviral activities of Ginkgo biloba (GB) leaves extract and eco-friendly free silver nanoparticles (Ag NPs) against the MERS-CoV (Middle East respiratory syndrome-coronavirus) and HCoV-229E (human coronavirus 229E), as well as isolation and identification of phytochemicals from GB. Different solvents and high-performance liquid chromatography (HPLC) were used to extract and identify flavonoids and phenolic compounds from GB leaves. The green, silver nanoparticle synthesis was synthesized from GB leaves aqueous extract and investigated for their possible effects as anti-coronaviruses MERS-CoV and HCoV-229E using MTT assay protocol. To verify the synthesis of Ag NPs, several techniques were employed, including X-ray diffraction (XRD), scan, transmission electron microscopy, FT-IR, and UV–visible spectroscopy. The highest contents of flavonoids and phenolic compounds were recorded for acetone, methanol, and ethanol as mixtures with water, in addition to pure water. HPLC flavonoids were detected as apegenin, luteolin, myricetin, and catechin, while HPLC phenolic compounds were pyrogallol, caffeic acid, gallic acid, and ellagic acid. In addition, our results revealed that Ag NPs were produced through the shift from yellow to dark brown. TEM examination of Ag NPs revealed spherical nanoparticles with mean sizes ranging from 5.46 to 19.40 nm and an average particle diameter of 11.81 nm. A UV–visible spectrophotometric investigation revealed an absorption peak at λ max of 441.56 nm. MTT protocol signified the use of GB leaves extract as an anti-coronavirus to be best from Ag NPs because GB extract had moderate anti-MERS-CoV with SI = 8.94, while had promising anti-HCov-229E, with an SI of 21.71. On the other hand, Ag NPs had a mild anti-MERS-CoV with SI = 4.23, and a moderate anti-HCoV-229E, with an SI of 7.51.

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Section: Introductionmentioning

confidence: 99%

Identifying the Anti-MERS-CoV and Anti-HcoV-229E Potential Drugs from the Ginkgo biloba Leaves Extract and Its Eco-Friendly Synthesis of Silver Nanoparticles

et al. 2023

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“…Most acylglycerides (99%) constitute argan oil, whether edible or cosmetic grade (primarily triglycerides). The remaining one percent, known as unsaponifiable matter, comprises xanthophylls, triterpene alcohols, carotenes, and tocopherols ( Charrouf and Guillaume, 1999 ; ZeinEldin et al, 2023 ). The primary fatty acids that constitute acylglycerides are oleic and linoleic acids, which account for 43%–49% and 29%–36%, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Even though argan seed cake has primarily been used for animal feed ( Cherki et al, 2006 ), it has also been suggested as a natural moisturizer and exfoliator for use in commercial shampoos and lotions to heal wounds, sprains, and scabies topically. Recent studies show that the ethanol extract of argan seed cake may be utilized as a natural lightening and pharmacological therapy for treating hyperpigmentation issues because of its effects on melanogenesis ( Bourhim et al, 2018 ; Bourhim et al, 2021 ; ZeinEldin et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

In vitro and in silico biopotentials of phytochemical compositions and antistaphylococcal and antipseudomonal activities of volatile compounds of Argania spinosa (L.) seed oil

Almuhayawi,

Alruhaili,

Gattan

et al. 2024

Front. Bioeng. Biotechnol.

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Active components in medicinal plants provide unlimited useful and traditional medicines. Antimicrobial activities are found in secondary metabolites in plant extracts such as argan oil. This experimental investigation aims to determine argan oil’s volatile compounds and examine their in vitro antimicrobial properties. In silico simulations, molecular docking, pharmacokinetics, and drug-likeness prediction revealed the processes underlying the in vitro biological possessions. Gas chromatography–mass spectrometry (GC/MS) was used to screen argan oil’s primary components. In silico molecular docking studies were used to investigate the ability of the selected bioactive constituents of argan oil to act effectively against Pseudomonas aeruginosa and Staphylococcus aureus (S. aureus) isolated from infections. The goal was to study their ability to interact with both bacteria’s essential therapeutic target protein. The 21 chemicals in argan oil were identified by GC/MS. Docking results for all compounds with S. aureus and P. aeruginosa protease proteins ranged from −5 to −9.4 kcal/mol and −5.7 to −9.7 kcal/mol, respectively, compared to reference ligands. Our docking result indicates that the 10-octadecenoic acid, methyl ester was the most significant compound with affinity scores of −9.4 and −9.7 kcal/mol for S. aureus and P. aeruginosa proteins, respectively. The minimal bactericidal concentration (MBC) and minimal inhibitory concentration (MIC) of argan oil were 0.7 ± 0.03 and 0.5 ± 0.01 for S. aureus and 0.4 ± 0.01 and 0.3 ± 0.02 for P. aeruginosa, respectively. We confirmed the antimicrobial properties of argan oil that showed significant growth inhibition for S. aureus and P. aeruginosa.

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“…Most acylglycerides (99%) make up argan oil, whether edible or cosmetic grade (primarily triglycerides). The remaining one percent, known as unsaponi able matter, comprises xanthophylls, triterpene alcohols, carotenes, and tocopherols 10,34 . The primary fatty acids that makeup acylglycerides are oleic and linoleic acid, which account for 43-49% and 29-36%, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies show that the ethanol extract of argan seed cake may be utilized as a natural lightening and pharmacological therapy for treating hyperpigmentation issues because of its effects on melanogenesis. [8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

Argania spinosa (L.) essential oil: Phytochemical Characterization, Molecular Docking, Anti-staphylococcal, and Anti-pseudomonal Activity

Almuhayawi

Alruhaili

Gattan

et al. 2023

Preprint

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Medicinal plants exemplify an infinite source of effective remedies and traditional thanks to the various active components they contain. Secondary metabolites current in plant extracts, for instance, essential oils, have significant pharmacological properties, including antimicrobic effects. This experimental investigation aims to determine Argan oil’s volatile compounds and exanimate their in vitro antimicrobial properties. In silico simulations, molecular docking and pharmacokinetics (absorption, distribution, metabolism, excretion, and toxicity (ADMET) and drug-likeness prediction revealed the processes underlying the in vitro biological possessions. Gas chromatography–mass spectrophotometry (GC-MS) was used for chemical screening of the main components of Argan oil. In silico molecular docking studies were applied as an attempt to investigate the ability of selected bioactive constituents of extra argan oil to act as effective against Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus) isolated from ear Infections, aiming to study their ability to interact with protease protein of S. aureus (PDB id: 5MM8) and P. aeruginosa (PDB id: 7M1M) essential therapeutic target protein. GC/MS analysis showed that Argan oil components were 21 compounds such as Oleic Acid, Ethyl Oleate, 10-Octadecenoic acid, and methyl ester. All compounds docked with protease protein of S. aureus and P. aeruginosa, the docking result values ranged from − 5 to -9.4 kcal/mol for protease protein of S. aureus and from − 5.7 to -9.7 kcal/mol for protease protein of P. aeruginosa compared with reference ligands. On the other hand, the studied Gram-positive bacteria were more influenced by the oil extracts under study than the Gram-negative bacteria. MIC illustrated that Argan seed oil inhibits S. aureus and P. aeruginosa. MBC ranged from 0.4 to 0.7 mg/ml. These outcomes afford scientific authentication of the traditional usages of this plant and recommend its impending application as natural medications.

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Diversity and Distribution Characteristics of Viruses from Soda Lakes

Cited by 9 publications

References 45 publications

Identifying the Anti-MERS-CoV and Anti-HcoV-229E Potential Drugs from the Ginkgo biloba Leaves Extract and Its Eco-Friendly Synthesis of Silver Nanoparticles

Identifying the Anti-MERS-CoV and Anti-HcoV-229E Potential Drugs from the Ginkgo biloba Leaves Extract and Its Eco-Friendly Synthesis of Silver Nanoparticles

In vitro and in silico biopotentials of phytochemical compositions and antistaphylococcal and antipseudomonal activities of volatile compounds of Argania spinosa (L.) seed oil

Argania spinosa (L.) essential oil: Phytochemical Characterization, Molecular Docking, Anti-staphylococcal, and Anti-pseudomonal Activity

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