Diversity and Differential Expression of MicroRNAs in the Human Skeletal Muscle with Distinct Fiber Type Composition

Zhelankin, Andrey V.; Iulmetova, Liliia N.; Ahmetov, Ildus I.; Генерозов, Э. В.; Шарова, Е. И.

doi:10.3390/life13030659

Cited by 10 publications

(6 citation statements)

References 104 publications

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“…Only the increased miR-206-3p level was affected in Wt mice and did not change in Tg mice. More importantly, the increase in miR-206 levels has been observed in power athletes with fast-twitch fiber predominance [35]. Given the training effect in Wt mice and training-induced phenotypic maladaptation in Tg mice, our data likely suggest that increased miR-206-3p levels may play an important role in resistance exercise-induced hypertrophy and may be linked to the interference effect.…”

Section: Discussionmentioning

confidence: 51%

Adaptation of Resistance Training is impaired in muscle-specific PGC-1α overexpressing mice

ZHANG,

SONG,

BAI

et al. 2024

Preprint

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Resistance training (RT) is important for skeletal muscle health. However, compared with RT implemented alone, concurrent training can attenuate RT adaptations. We verify the effects of RT on muscle-enriched or hypertrophy-related miRNAs, mammalian target of rapamycin complex 1 (mTORC1) anabolic signaling proteins, and oxidative metabolism-related proteins in mice with different endurance muscle Phenotype. In the present study, we found that muscle-specific overexpression of PGC-1alpha attenuated RT adaptations and resulted in different expression of miR-222-3p and miR-206-3p after RT. The expression level of miR-206-3p and miR-222-3p in Wt+ mice (wild-type trained mice) was significantly higher than that in Tg+ mice (MCK-PGC-1α transgenic trained mice). This suggested that discordant miR-222-3p and miR-206-3p expression levels after RT may play a role in the mechanism contributing to the interference effects. With regard to protein expression,there was no difference in mTORC1 between the genotypes. Furthermore, the levels of parameters for oxidative metabolism were significantly higher in Tg mice than in Wt mice. Our study indicated miR-222-3p and miR-206-3pas novel molecules that could provide a new research direction into the mechanisms underlying the concurrent training effect.

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Section: Discussionmentioning

confidence: 51%

Adaptation of Resistance Training is impaired in muscle-specific PGC-1α overexpressing mice

ZHANG,

SONG,

BAI

et al. 2024

Preprint

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“…In their study including 28 juvenile Iberian pigs fed either a control or a high-fructose, high-fat (HFF) diet for 10 weeks, H.C. Spooner et al [ 70 ] demonstrated that the HFF diet resulted in decreased IMCL fat content and in the formation of less oxidative skeletal muscle phenotype, reflecting the disturbed ability of skeletal muscles to use lipids as a fuel. These changes were similar to the effects of detraining or muscle atrophy, indicating the reduced capacity of muscles to perform endurance-type exercises [ 71 ]. These effects may be followed by a long-term decrease in muscle mass and strength.…”

Section: Skeletal Muscles In Obesitymentioning

confidence: 68%

Possible Mechanisms Linking Obesity, Steroidogenesis, and Skeletal Muscle Dysfunction

Sheptulina,

Antyukh,

Kiselev

et al. 2023

Life

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Increasing evidence suggests that skeletal muscles may play a role in the pathogenesis of obesity and associated conditions due to their impact on insulin resistance and systemic inflammation. Skeletal muscles, as well as adipose tissue, are largely recognized as endocrine organs, producing biologically active substances, such as myokines and adipokines. They may have either beneficial or harmful effects on the organism and its functions, acting through the endocrine, paracrine, and autocrine pathways. Moreover, the collocation of adipose tissue and skeletal muscles, i.e., the amount of intramuscular, intermuscular, and visceral adipose depots, may be of major importance for metabolic health. Traditionally, the generalized and progressive loss of skeletal muscle mass and strength or physical function, named sarcopenia, has been thought to be associated with age. That is why most recently published papers are focused on the investigation of the effect of obesity on skeletal muscle function in older adults. However, accumulated data indicate that sarcopenia may arise in individuals with obesity at any age, so it seems important to clarify the possible mechanisms linking obesity and skeletal muscle dysfunction regardless of age. Since steroids, namely, glucocorticoids (GCs) and sex steroids, have a major impact on the amount and function of both adipose tissue and skeletal muscles, and are involved in the pathogenesis of obesity, in this review, we will also discuss the role of steroids in the interaction of these two metabolically active tissues in the course of obesity.

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“…In previous studies, miR-1, miR-133a, miR-133b and miR-206 have been reported to be myomiRs, i.e., miRNAs that are highly expressed specifically in muscle ( 43 – 46 ). In particular, miR-206 exhibits muscle-specific expression characteristics ( 43 , 45 , 47 ), and miR-133b is downregulated in the plasma of patients with sarcopenia ( 24 ). A previous study showed that the expression levels of mature miRNAs and their precursors are not positively correlated ( 14 ).…”

Section: Discussionmentioning

confidence: 99%

Identifying the potential therapeutic effects of miR‑6516 on muscle disuse atrophy

Jung,

Juang,

Gwon

et al. 2024

Mol Med Rep

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Muscle atrophy is a debilitating condition with various causes; while aging is one of these causes, reduced engagement in routine muscle-strengthening activities also markedly contributes to muscle loss. Although extensive research has been conducted on microRNAs (miRNAs/miRs) and their associations with muscle atrophy, the roles played by miRNA precursors remain underexplored. The present study detected the upregulation of the miR-206 precursor in cell-free (cf)RNA from the plasma of patients at risk of sarcopenia, and in cfRNAs from the muscles of mice subjected to muscle atrophy. Additionally, a decline in the levels of the miR-6516 precursor was observed in mice with muscle atrophy. The administration of mimic-miR-6516 to mice immobilized due to injury inhibited muscle atrophy by targeting and inhibiting cyclin-dependent kinase inhibitor 1b (Cdkn1b). Based on these results, the miR-206 precursor appears to be a potential biomarker of muscle atrophy, whereas miR-6516 shows promise as a therapeutic target to alleviate muscle deterioration in patients with muscle disuse and atrophy.

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Diversity and Differential Expression of MicroRNAs in the Human Skeletal Muscle with Distinct Fiber Type Composition

Cited by 10 publications

References 104 publications

Adaptation of Resistance Training is impaired in muscle-specific PGC-1α overexpressing mice

Adaptation of Resistance Training is impaired in muscle-specific PGC-1α overexpressing mice

Possible Mechanisms Linking Obesity, Steroidogenesis, and Skeletal Muscle Dysfunction

Identifying the potential therapeutic effects of miR‑6516 on muscle disuse atrophy

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