Diversity and composition of vaginal microbiota of pregnant women at risk for transmitting Group B Streptococcus treated with intrapartum penicillin

Roesch, Luiz Fernando Würdig; Silveira, Rita de Cássia dos Santos; Corso, Andrea; Dobbler, Priscila Caroline Thiago; Mai, Volker; Rojas, Bruna Schafer; Laureano, Álvaro Macedo; Procianoy, Renato S.

doi:10.1371/journal.pone.0169916

Cited by 22 publications

(26 citation statements)

References 36 publications

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“…Despite their widespread use, there are few published data on the potential effects of antibiotics on the infant and maternal microbiome during the process of labour and delivery at term gestation. [37][38][39][40][41][42][43][44] To our knowledge, no large cohort study has examined the effect of specific classes of antibiotics on the developing infant intestinal microbiota of full-term infants. This is relevant in practice because different classes of antibiotics are Figure 3.…”

Section: Main Findingsmentioning

confidence: 99%

“…This finding is similar to those of previous studies focused on the use of maternal antibiotics in general, particularly with the decrease in Bacteroides and Bifidobacterium. [38][39][40][41][42][43]46,47 One study 40 observed significant effects of GBS prophylaxis on the infant gut microbiome at 6 months among 150 pregnancies for members of Clostridiaceae, Ruminococcoceae and Enterococcaceae whereas another 41 (n = 83) found lower levels of Bifidobacterium and Escherichia at 12 weeks of life in infants of mothers exposed to intrapartum antibiotics.…”

Section: Main Findingsmentioning

confidence: 99%

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Specific class of intrapartum antibiotics relates to maturation of the infant gut microbiota: a prospective cohort study

Coker

Hoen

Dade

et al. 2019

BJOG

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Objective To evaluate the potential impact of intrapartum antibiotics, and their specific classes, on the infant gut microbiota in the first year of life. Design Prospective study of infants in the New Hampshire Birth Cohort Study (NHBCS). Settings Rural New Hampshire, USA. Population or sample Two hundred and sixty-six full-term infants from the NHBCS. Methods Intrapartum antibiotic use during labour and delivery was abstracted from medical records. Faecal samples collected at 6 weeks and 1 year of age were characterised by 16S rRNA sequencing, and metagenomics analysis in a subset of samples. Exposures Maternal exposure to antibiotics during labour and delivery. Main outcome measure Taxonomic and functional profiles of faecal samples. Results Infant exposure to intrapartum antibiotics, particularly to two or more antibiotic classes, was independently associated with lower microbial diversity scores as well as a unique bacterial community at 6 weeks (GUnifrac, P = 0.02). At 1 year, infants in the penicillin-only group had significantly lower α diversity scores than infants not exposed to intrapartum antibiotics. Within the first year of life, intrapartum exposure to penicillins was related to a significantly lower increase in several taxa including Bacteroides, use of cephalosporins was associated with a significantly lower rise over time in Bifidobacterium and infants in the multi-class group experienced a significantly higher increase in Veillonella dispar. Conclusions Our findings suggest that intrapartum antibiotics alter the developmental trajectory of the infant gut microbiome, and specific antibiotic types may impact community composition, diversity and keystone immune training taxa.

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Section: Main Findingsmentioning

confidence: 99%

Section: Main Findingsmentioning

confidence: 99%

Specific class of intrapartum antibiotics relates to maturation of the infant gut microbiota: a prospective cohort study

Coker

Hoen

Dade

et al. 2019

BJOG

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“…Those subjects called hereinafter "non-spontaneous preterm" group, were used as a second control because they present a microbial community that might not be associated with spontaneous preterm delivery but have a better match in terms of gestational age with the spontaneous preterm group. All pregnant women sampled on this work had vaginal swab (Sterile Specimen Collection Swabs to collect specimens from soft tissue surfaces-Labor swab®) collected up to 4 hours before labor begins, as described by Roesch and colleagues [27]. Collected swab samples were stored at − 80°C until DNA extraction.…”

Section: Experimental Designmentioning

confidence: 99%

“…Microbial DNA extraction, 16S rRNA amplification and sequencing, and data processing Microbial DNA was extracted from frozen swab samples as previously described by Roesch et al [27]. All DNA samples were kept at − 20°C until use in PCR reactions.…”

Section: Experimental Designmentioning

confidence: 99%

Defining microbial biomarkers for risk of preterm labor

et al. 2019

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Preterm birth remains the main contributor to early childhood mortality. The vaginal environment, including microbiota composition, might contribute to the risk of preterm delivery. Alterations in the vaginal microbial community structure might represent a risk factor for preterm birth. Here, we aimed to (a) investigate the association between preterm birth and the vaginal microbial community and (b) identify microbial biomarkers for risk of preterm birth. Microbial DNA was isolated from vaginal swabs in a cohort of 69 women enrolled at hospital admission for their delivery. Microbiota was analyzed by high-throughput 16S rRNA sequencing. While no differences in microbial diversity measures appeared associated with the spontaneous preterm and full-term outcomes, the microbial composition was distinct for these groups. Differential abundance analysis showed Lactobacillus species to be associated with full-term birth whereas an unknown Prevotella species was more abundant in the spontaneous preterm group. Although we studied a very miscegenated population from Brazil, our findings were similar to evidence pointed by other studies in different countries. The role of Lactobacillus species as a protector in the vaginal microbiome is demonstrated to be also a protector of spontaneous preterm outcome whereas the presence of pathogenic species, such as Prevotella spp., is endorsed as a factor of risk for spontaneous preterm delivery.

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“…Importantly, shifts in the gut microbiome across pregnancy can persist for at least 4 weeks postpartum (Koren et al, ), thus any perturbations such as the use of antibiotics can influence inflammation, dysregulate the gut‐brain‐axis, and potentiate the risk for adverse outcomes. Antibiotics administered during pregnancy have been shown to significantly decrease the presence of Lactobacillus , the dominant vaginal microbe that prevents infections by maintaining the microbial integrity of the vaginal environment (Khan et al, ; Miller, Beasley, Dunn, & Archie, ; Roesch et al, ). A shift in the abundance of vaginal Lactobacillus is not only more prevalent in women with bacterial infections, but also makes women more susceptible to bacterial infections (Kacerovsky et al, ) and increases the chances of receiving additional antibiotic therapy.…”

Section: Introductionmentioning

confidence: 99%

Maternal peripartum antibiotic exposure and the risk of postpartum depression

Murphy

Paul

Dunlop

et al. 2018

Research in Nursing & Health

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Postpartum depression is a relatively common occurrence that may carry lifelong consequences. Also common is the exposure of pregnant and postpartum women in the United States to antibiotics, especially during the peripartum period. Antibiotic exposure is known to alter gut microbial composition and structure, contributing to a dysbiotic, or imbalanced gut microbiome, and is a mechanism suggested for the increased risk of depressive symptoms following antibiotic exposure in non-pregnant, non-postpartum populations. We sought to determine the relationship between antibiotic exposure intrapartum through the first 14 days postpartum and depressive symptoms within the first 6-months postpartum. In this secondary data analysis, 124 pregnant women from a prospective cohort were enrolled into the study and provided demographic characteristics and health history. Participants completed questionnaires including the Edinburgh Postnatal Depression Scale between 32-36 weeks, and 1- and 2-weeks and 1-, 2-, 3-, and 6-months postpartum. Clinical measures including hours in labor, perineal injury, and mode of giving birth were ascertained by medical record abstraction. Antibiotic exposure was identified by self-report at 1- and 2-weeks postpartum. Antibiotic exposure was found to be independently predictive of postpartum depressive symptoms at 1-month (Beta = 0.43; CI [0.01, 0.86]) and 2-months (Beta = 0.51; CI [0.08, 0.94]) postpartum after controlling for baseline predictors. The relationship between antibiotic exposure and postpartum depressive symptoms did not maintain significance at 3- or 6-months postpartum. Additional studies are needed to further investigate the relationship between onset and duration of antibiotic exposure, the peripartum gut microbiome, and the risk for postpartum depressive symptoms.

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Diversity and composition of vaginal microbiota of pregnant women at risk for transmitting Group B Streptococcus treated with intrapartum penicillin

Cited by 22 publications

References 36 publications

Specific class of intrapartum antibiotics relates to maturation of the infant gut microbiota: a prospective cohort study

Specific class of intrapartum antibiotics relates to maturation of the infant gut microbiota: a prospective cohort study

Defining microbial biomarkers for risk of preterm labor

Maternal peripartum antibiotic exposure and the risk of postpartum depression

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