Diversity and communities of culturable endophytic fungi from different tree peonies (geoherbs and non-geoherbs), and their biosynthetic potential analysis

Abstract

To know more about the potential roles of endophytic fungi in the formation mechanism of Daodi medicinal material, diversity and communities of culturable endophytic fungi in three types of tree peonies were investigated. Endophytic fungi of three types of tree peonies were isolated and identified. The diversity was analyzed. Bayesian trees constructed by MrBayes 3.2.6 after phylogenetic analysis of the ITS sequences. The endophytic fungi potential for synthesis of natural products was assessed by means of detecting NRPS and PKS gene sequences. In total, 364 endophytic fungi isolates representing 26 genera were recovered from Paeonia ostii 'Feng Dan', Paeonia ostii 'Luoyang Feng Dan', and Paeonia suffruticosa 'Luoyang Hong'. More culturable endophytic fungi appeared in P. suffruticosa 'Luoyang Hong' (206) compared with P. ostii 'Feng Dan' (60) and P. ostii 'Luoyang Feng Dan' (98). The fungal community of P. ostii 'Feng Dan' had the highest richness and diversity. PKSs and NRPS detection rates of endophytic fungi from P. ostii 'Feng Dan' are both the highest among the three types of tree peonies. Results indicate that endophytic fungus is an important factor of Daodi Cortex Moutan forming, and endophytic fungi in peony are related to genuineness of Cortex Moutan.

Endophytic Fungi: Biological Control and Induced Resistance to Phytopathogens and Abiotic Stresses

“…was recently analyzed and different genera of fungi were identified. The most abundant among them were Fusarium, Phoma, Alternaria and Pestalotiopsis [ 56 ]. Other examples can also be found, such as Coniothyrium species isolated from the cortex of Picea abies branches [ 57 ], Asteromella fungus isolated from the inside of Quercus emoryi leaves [ 58 ], Phoma isolated from wheat leaves [ 59 ] and Aspergillus, Curvularia lunata, Fusarium, Penicillium and Trichoderma isolated from sunflower seeds [ 60 ].…”

“…The population dynamics of endophytic fungi may be related to some host properties such as chemical composition [ 63 ], physiological conditions [ 64 ], geographic distribution, plant age and ecological conditions, including altitude and precipitation [ 65 ]. Ecological or environmental conditions such as temperature, humidity, lighting, geographic location and vegetation significantly affect the distribution pattern and population structure of endophytic fungi [ 56 ]. For example, one or two species are predominantly endophytic in a given host, while others are uncommon [ 51 , 66 ].…”

The Diversity Analysis and Functional Prediction of Endophytic Fungi Community of Gymnadenia Conopsea from Different Altitudes in Tibet

“…Endophytes are a group of microorganisms with various morphologies, mainly including endophytic fungi and bacteria, that can enter into plants through the root cortex, wounds, or stomata and reside in different tissues or organs without causing any harm to the host plants ( Yang et al 2018). Moreover, exploring the relationship between plants and endophytic fungi is also important, and few studies have been performed to determine whether endophytes are related to the synthesis of bioactive compounds in G. conopsea.…”

“…). Endophytic fungi from medicinal plants can synthesize many of the same or similar bioactive substances as their host plants(Yang et al 2018). Relevant reports have shown that endophytes in medicinal plants have pharmacologically active compounds, such as antioxidants, antibacterial compounds, and tumor growth inhibitors(Sarsaiya et al 2019), whichMa et al (2015) indicated was related to the diversity of endophytic fungal metabolites.…”

Obtenção de extratos enzimáticos enriquecidos em proteases a partir de fungos associados a caules e folhas de mandioca visando a digestão do glúten

“…Durante essa associação, a planta fornece nutrição e refúgio aos fungos. Em contrapartida, os mesmos promovem o controle de pragas, a tolerância a seca, a decomposição foliar, auxiliam no fluxo de nutrientes, pH e umidade e fornecem alguns metabólitos secundários importantes para o crescimento de plantas, caracterizando, assim, essa relação como uma simbiose de mutualismo (LI et al, 2018;MANGANYI et al, 2018;YANG et al, 2018;FERNANDES et al, 2015).…”