Diversity and biological activities of medicinal plants of Santiago island (Cabo Verde)

Romeiras, Maria M.; Essoh, Anyse Pereira; Catarino, Sílvia; Silva, Joceline; Lima, Katelene; Varela, Eromise; Moura, Mónica; Gómes, Isildo; Duarte, Maria Cristina; Duarte, Paula

doi:10.1016/j.heliyon.2023.e14651

Cited by 9 publications

(3 citation statements)

References 67 publications

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“…However, these drugs are associated with gastrointestinal disturbance, abdominal pain, and flatulence [ 72 ]. Thus, many studies have focused on identifying alternative α-amylase and α-glucosidase inhibitors, such as plant extracts [ 73 ] or food derived bioactive peptides [ 30 , 71 , 72 , 74 , 75 , 76 ], that may simultaneously have inhibitory activity and a low incidence of undesirable side effects.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Multifaceted Potential of a Peptide Fraction Derived from Saccharomyces cerevisiae Metabolism: Antimicrobial, Antioxidant, Antidiabetic, and Anti-Inflammatory Properties

Branco,

Maurício,

Costa

et al. 2023

Antibiotics

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The rising demand for minimally processed, natural, and healthier food products has led to the search for alternative and multifunctional bioactive food components. Therefore, the present study focuses on the functional proprieties of a peptide fraction derived from Saccharomyces cerevisiae metabolism. The antimicrobial activity of the peptide fraction is evaluated against various foodborne pathogens, including Candida albicans, Candida krusei, Escherichia coli, Listeria monocytogenes, and Salmonella sp. The peptide fraction antioxidant properties are assessed using FRAP and DPPH scavenging capacity assays. Furthermore, the peptide fraction’s cytotoxicity is evaluated in colorectal carcinoma and normal colon epithelial cells while its potential as an antidiabetic agent is investigated through α-amylase and α-glucosidase inhibitory assays. The results demonstrate that the 2–10 kDa peptide fraction exhibits antimicrobial effects against all tested microorganisms, except C. krusei. The minimal inhibitory concentration for E. coli, L. monocytogenes, and Salmonella sp. remains consistently low, at 0.25 mg/mL, while C. albicans requires a higher concentration of 1.0 mg/mL. Furthermore, the peptide fraction displays antioxidant activity, as evidenced by DPPH radical scavenging activity of 81.03%, and FRAP values of 1042.50 ± 32.5 µM TE/mL at 1.0 mg/mL. The peptide fraction exhibits no cytotoxicity in both tumor and non-tumoral human cells at a concentration up to 0.3 mg/mL. Moreover, the peptide fraction presents anti-inflammatory activity, significantly reducing the expression of the TNFα gene by more than 29.7% in non-stimulated colon cells and by 50% in lipopolysaccharide-stimulated colon cells. It also inhibits the activity of the carbohydrate digestive enzymes α-amylase (IC50 of 199.3 ± 0.9 µg/mL) and α-glucosidase (IC20 of 270.6 ± 6.0 µg/mL). Overall, the findings showed that the peptide fraction exhibits antibacterial, antioxidant, anti-inflammatory, and antidiabetic activity. This study represents a step forward in the evaluation of the functional biological properties of S. cerevisiae bioactive peptides.

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Section: Discussionmentioning

confidence: 99%

Exploring the Multifaceted Potential of a Peptide Fraction Derived from Saccharomyces cerevisiae Metabolism: Antimicrobial, Antioxidant, Antidiabetic, and Anti-Inflammatory Properties

Branco,

Maurício,

Costa

et al. 2023

Antibiotics

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“…In fact, the extraction yield depends on the extraction method and the solvent used for extraction. The use of aqueous mixtures containing ethanol, methanol, acetone, and ethyl acetate provides good results in the extraction of natural products from plant matrices; however, ethanol is recognized as a good solvent for polyphenol extraction and is safe for human consumption [11,38].…”

Section: Discussionmentioning

confidence: 99%

“…The estimated percentage of the African population's reliance on traditional forms of medicine to satisfy their healthcare needs is still extremely high [9]. In Cabo Verde, traditional medicine plays an important role in the health system of the country since rural communities still have limited access to conventional medicine [10,11].…”

Section: Introductionmentioning

confidence: 99%

Quality, Safety and Biological Studies on Campylanthus glaber Aerial Parts

Lima,

Malmir,

Camões

et al. 2023

Pharmaceuticals

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In Cabo Verde, several endemic species are used in traditional medicine. However, no scientific studies have been conducted on the quality, efficacy, and safety of most of these plants. This study focused on establishing the botanical and chemical identification parameters required for a quality monograph of Campylanthus glaber Benth. aerial parts, a medicinal plant of Cabo Verde traditionally used to treat fever and muscular pain. In addition, in vitro antioxidant and antihyperglycemic activity, cytotoxicity, and genotoxicity were assessed for this medicinal plant. Optical microscopy, LC/UV-DAD-ESI/MS, and colorimetric assays were used for botanical, chemical, and biological studies, respectively. Cytotoxicity was assessed by the MTT assay with HepG2 cells, and genotoxicity by the Ames test. Microscopically, the xeromorphic leaf of C. glaber presents a thick cuticle (13.6–25.5 µm), thick-walled epidermal cells, anomocytic-type stomata, glandular trichomes (stalk length = 49.4–120.8 µm), and idioblasts containing calcium oxalate microcrystals. The chemical screening of aqueous and hydroethanolic extracts of this medicinal plant revealed the presence of organic acids, iridoids, phenylethanoids, and flavonoids as the main classes of marker compounds, with malic acid, citric acid, and verbascoside being the main marker compounds identified. Both extracts showed similar LC/UV-DAD/ESI-MS qualitative profiles and DPPH radical scavenger activity (IC50 = 130.9 ± 1.4; 134.3 ± 3.1 µg/mL). The hydroethanolic extract inhibited both α-amylase and α-glucosidase enzymes in a dose-dependent manner. Both extracts showed no cytotoxicity (up to 1000 µg/mL) by the MTT assay and no genotoxic potential with or without metabolic activation up to 5 mg /plate. The results obtained are an important contribution to the monographic quality assessment of C. glaber aerial parts and suggest that this medicinal plant may be safe and potentially used as an herbal drug raw material for pharmaceutical purposes.

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