Diversity and Bioactivity of Marine Bacteria Associated with the Sponges Candidaspongia flabellata and Rhopaloeides odorabile from the Great Barrier Reef in Australia

Brinkmann, Candice M.; Kearns, Philip S.; Evans-Illidge, Elizabeth; Kurtböke, D. İpek

doi:10.3390/d9030039

Cited by 12 publications

(7 citation statements)

References 71 publications

(104 reference statements)

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“…Several of these taxa have been highlighted before for their antimicrobial activity. Actinobacteria and Proteobacteria are considered the most prolific sources of bioactive secondary metabolites with a wide spectrum of bioactivities among marine microorganisms [ 59 , 60 , 61 , 62 , 63 ]. However, only one strain, identified as Rhodococcus erythropolis , possessed anti-MRSA potential.…”

Section: Discussionmentioning

confidence: 99%

“…Yet, these antibiotic resistance phenotypes did not match with the antimicrobial activity screening results, which could be due to a lack of induction of gene expression under the tested conditions. Diverse fermentation conditions using various nutrients and treatments (e.g., co-culturing) may be of great value in activating silent genes in the search for bioactive secondary metabolites [ 41 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

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Bioactivity Screening and Gene-Trait Matching across Marine Sponge-Associated Bacteria

et al. 2021

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Marine sponges harbor diverse microbial communities that represent a significant source of natural products. In the present study, extracts of 21 sponge-associated bacteria were screened for their antimicrobial and anticancer activity, and their genomes were mined for secondary metabolite biosynthetic gene clusters (BGCs). Phylogenetic analysis assigned the strains to four major phyla in the sponge microbiome, namely Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. Bioassays identified one extract with anti-methicillin-resistant Staphylococcus aureus (MRSA) activity, and more than 70% of the total extracts had a moderate to high cytotoxicity. The most active extracts were derived from the Proteobacteria and Actinobacteria, prominent for producing bioactive substances. The strong bioactivity potential of the aforementioned strains was also evident in the abundance of BGCs, which encoded mainly beta-lactones, bacteriocins, non-ribosomal peptide synthetases (NRPS), terpenes, and siderophores. Gene-trait matching was performed for the most active strains, aiming at linking their biosynthetic potential with the experimental results. Genetic associations were established for the anti-MRSA and cytotoxic phenotypes based on the similarity of the detected BGCs with BGCs encoding natural products with known bioactivity. Overall, our study highlights the significance of combining in vitro and in silico approaches in the search of novel natural products of pharmaceutical interest.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Bioactivity Screening and Gene-Trait Matching across Marine Sponge-Associated Bacteria

et al. 2021

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“…are generally recovered from sponges. In addition to Pseudovibrio, members of the genera Ruegeria and Microbulbifer were also among the most abundant isolates from all sponge specimens, and these two genera have also been frequently reported from other cultivation studies of bacteria from marine sponges [56,57]. Ruegeria has been associated to facilitating cell-to-cell communication between bacteria and the sponge host [58,59], but the fact that these bacteria were not detected in the original samples casts some doubt on this hypothesis.…”

Section: Discussionmentioning

confidence: 74%

Cultivation of Sponge-Associated Bacteria from Agelas sventres and Xestospongia muta Collected from Different Depths

et al. 2019

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Sponge-associated bacteria have been mostly cultured from shallow water (≤30 m) sponges, whereas only few studies targeted specimens from below 30 m. This study assessed the cultivability of bacteria from two marine sponges Xestospongia muta and Agelas sventres collected from shallow (<30 m), upper mesophotic (30–60 m), and lower mesophotic (60–90 m) reefs. Sponge-associated bacteria were cultivated on six different media, and replicate plates were used to pick individual colonies or to recover the entire biomass. Prokaryotic community analysis was conducted using Illumina MiSeq sequencing of 16S rRNA gene amplicons. A total of 144 bacterial isolates were picked following a colony morphology coding scheme and subsequently identified by 16S rRNA gene sequence analysis. Sponge individuals at each depth-range harboured specific cultivable bacteria that were not retrieved from specimens collected at other depths. However, there were substantial differences in the number of colonies obtained for replicate sponges of the same species. In addition, source of inoculum and cultivation medium had more impact on the cultured prokaryotic community than sample collection depth. This suggests that the “plate count anomaly” is larger than differences in sponge-associated prokaryotic community composition related to depth.

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“…Moreover, also therapeutic applications have been improved, and many sponge endosymbionts have been proved able to produce cytotoxic compounds against several forms of carcinoma [217]. An interesting approach in the study of bioactive molecules from associated bacteria is the detection and amplification of specific genes, which are involved in the biosynthetic pathways, as recently reported by Brinkmann et al [2], for the production of antimicrobial compounds by bacteria associated with the sponges Candidaspongia flabellata and Rhopaloeides odorabile.…”

Section: Biomedicalsmentioning

confidence: 99%

“…Associated bacteria support the defensive strategies of the host organism by producing secondary metabolites, in response to the environmental conditions of surrounding water. For this reason, associated bacterial communities have been firstly and deeply investigated for the discovery of new drugs, valid for the application in pharmaceutical and medical fields as antibacterial, antiviral, antifungal, and antiprotozoal functions [1][2][3][4]. In addition to biomedicals, among secondary metabolites of microbial origin, extracellular polymeric substances-including biosurfactants and exopolysaccharides-represent a class of eco-friendly compounds that were investigated since several decades for their advantageous applications in numerous and important fields.…”

Section: Introductionmentioning

confidence: 99%

Marine Invertebrates: Underexplored Sources of Bacteria Producing Biologically Active Molecules

Rizzo

Giudice

2018

Diversity

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The marine bioprospecting could be considered as a new phenomenon, and already potentially more promising than terrestrial one in terms of possible discovery of new compounds. The secondary metabolites produced by associated-bacteria are actually studied for their remarkable role in several fields, including agricultural, industrial, medical, and bioremediation strategies against different contaminants. The use of such renewable sources could be helpful in the streamlining of the patenting process for natural compounds of good quality, produced with lower energy costs and less impact on the environment. Anyway, more improvements in the research and application of bioactive compounds as alternative to the synthetic counterparts have to be carried out for the costs reduction and the large-scale production upgrading. The use of marine invertebrates could help to overcome these difficulties, as hotspots of microbial diversity and favorable matrix for the development of conditions stimulating the production of substances with special activities. This review will deal with the current accepted definitions and recent advances concerning: (i) the marine symbiotic relationships in which microorganisms and invertebrates are involved; (ii) the principal taxa of marine invertebrates that establish interactions with microorganisms, the biodiversity of these latter, and their role in the symbiosis; (iii) we address the state of current literature and knowledge about the bacterial associated communities specialized in biosurfactants (BSs) and extracellular polymeric substances (EPSs) production; and, (iv) their potential biotechnological applications reported still now.

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Diversity and Bioactivity of Marine Bacteria Associated with the Sponges Candidaspongia flabellata and Rhopaloeides odorabile from the Great Barrier Reef in Australia

Cited by 12 publications

References 71 publications

Bioactivity Screening and Gene-Trait Matching across Marine Sponge-Associated Bacteria

Bioactivity Screening and Gene-Trait Matching across Marine Sponge-Associated Bacteria

Cultivation of Sponge-Associated Bacteria from Agelas sventres and Xestospongia muta Collected from Different Depths

Marine Invertebrates: Underexplored Sources of Bacteria Producing Biologically Active Molecules

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