Diversity and Bioactivity of Endophytes From Angelica sinensis in China

Zhang, Xinguo; Guo, Si-Jia; Wang, Wenna; Wei, Guo-Xing; Ma, Guo-Yan; Ma, Xiangyuan

doi:10.3389/fmicb.2020.01489

Cited by 12 publications

(7 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was speculated that there was a phenomenon that the compounds that accumulated in the head transferred to the body and tail during the accumulation of effective components in different medicinal parts. Overall, the accumulation and distribution of compounds were consistent with the efficacy of different medicinal parts of A. sinensis and the accumulation of the types and contents of the compounds reached the maximum in October, which was consistent with the traditional harvesting period [ 19 ].…”

Section: Resultssupporting

confidence: 62%

The Dynamic Accumulation Rules of Chemical Components in Different Medicinal Parts of Angelica sinensis by GC-MS

Chen

Qiu

2022

Molecules

View full text Add to dashboard Cite

The chemical components and medicinal properties of different medicinal parts of Angelica sinensis are often used as medicine after being divided into the head, body and tail of Angelica sinensis. In this study, the chemical components of different medicinal parts in different periods were analyzed by GC-MS for the first time, and the differences of the accumulation rules of chemical components in different medicinal parts of Angelica sinensis were obtained. This study demonstrated that the differences of composition accumulation in different medicinal parts of Angelica sinensis were mainly reflected in the types and relative contents of compounds. The study found that the number of compounds in different medicinal parts of Angelica sinensis in each period were different and the change rules of the same compound in different medicinal parts were also different. The number of compounds in the tail of Angelica sinensis was the least in April, and the largest in October. The content of ligustilide in the body of Angelica sinensis was higher in April and was the highest in the tail in October. The relative content of butylidenephthalide in the head was the highest in October. The relative contents of senkyunolide A and butylphthalide in the head were decreased in October, while the contents in the body and tail increased, indicating that the compounds that accumulate in the head may transfer to the body and tail in later stages of growth. This study clarified the differences in the accumulation of chemical components in different medicinal parts of Angelica sinensis, which could provide a theoretical basis for the reasons for the differences of chemical components in the different medicinal parts.

show abstract

Section: Resultssupporting

confidence: 62%

The Dynamic Accumulation Rules of Chemical Components in Different Medicinal Parts of Angelica sinensis by GC-MS

Chen

Qiu

2022

Molecules

View full text Add to dashboard Cite

show abstract

“…The fungal internal transcribed spacer (ITS rDNA) was amplified using ITS1F (5′-CTTGGTCATTTAGAGGAAGTAA-3′) and ITS2R (5′-GCTGCGTTCTTCATCGATGC-3′). Amplification, purification, and sequencing library construction were referenced in Zhang X. G. et al (2020) . Purified amplicons were pooled in equimolar and paired-end sequenced (2 × 300) on an Illumina MiSeq platform (Illumina, San Diego, United States) according to the standard protocols by Majorbio Bio-Pharm Technology Co., Ltd. (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

Correlation in endophytic fungi community diversity and bioactive compounds of Sophora alopecuroides

Zhang

Wang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Sophora alopecuroides L. is a traditional Chinese medicine used for the treatment of several different disease states including bacillary dysentery and enteritis. But importantly, it also plays a role as an anti-tumor agent. That said, little is known about the role endophytes play regarding the clinically bioactive metabolites in S. alopecuroides. In order to explore the effects of endophytic fungi on the accumulation, quality, and correlation in the content of the medicinal compounds, the structural diversity of endophytic fungi in S. alopecuroides was analyzed. The relationship between endophytes and quinolizidine alkaloids (QAs), housed within the seeds of S. alopecuroides, which were interpreted based on established methods of high-throughput sequencing and high-performance liquid chromatography. A total of 1,034,418 effective sequence reads and 257 operational taxonomic units (OTUs) were obtained from 33 samples which were sourced from 11 different sampling sites and further classified into 9 phyla, 20 classes, 45 orders, 85 families, and 118 genera. Ascomycota was found to be the dominant phylum of endophytic fungi in S. alopecuroides, with a relative abundance ranging from 60.85 to 98.30%. Alternaria, Cladosporium, Filobasidium, and an unidentified Ascomycota were the core-shared endophytes, accounting for 49.96, 27.12, 14.83, and 7.88%, respectively. Correlation analysis showed that the Simpson’s diversity index of endophytic fungal community in S. alopecuroides was significantly positively correlated with the Oxymatrine (OMA) content in different areas, while the Chao and Shannoneven indexes were significantly negatively correlated with OMA. The endophytic fungi of Alternaria were positively correlated with the content of OMA, Oxysophocarpine (OSC), and total QAs. This study has mastered the endophytic fungi resources of S. alopecuroides, explored potential functional endophytic fungi, and provided a scientific basis for using biological fertilization strategies to improve the quality of S. alopecuroides.

show abstract

“…In this study, the antioxidant activity of 32 endophytic fungal EXEs was comprehensively evaluated by using PTIO and OH assays as indicators for scavenging reactive nitrogen, DPPH and ABTS assays as indicators for scavenging reactive oxygen species, and FRAP assay as an indicator for the ability to reduce “Fe 3+ .” The antioxidant activity of EXEs from eight endophytic fungal strains, AP-1, AP-4, AP-11, AP-12, AP-24, AP-42, AP-69, and AP-81, was evaluated ( Figure 3 ). According to known research results, the content of total phenols and total flavonoids is highly correlated with antioxidant activity ( Zhang et al, 2020 ). After testing the total phenols and total flavonoids of the EXEs of 8 strains of endophytic fungi, AP-1 and AP-4 contained relatively high total phenols and total flavonoids ( Figure 5B ).…”

Section: Discussionmentioning

confidence: 99%

A New Source of Diterpene Lactones From Andrographis paniculata (Burm. f.) Nees—Two Endophytic Fungi of Colletotrichum sp. With Antibacterial and Antioxidant Activities

Huang

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Endophytic fungi of medicinal plants are abundant, and their metabolites often have antioxidant, antibacterial, and antitumor effects and can produce secondary metabolites identical or similar to those of their hosts, which can mitigate the problem of insufficient supply of medicinal plants. In this study, we screened endophytic fungi for strains that produce the same diterpene lactones as Andrographis paniculata based on their biological activity. Firstly, the dominant group of endophytic fungi of Andrographis paniculata was screened and pathogenicity was studied using Koch’s rule. Secondly, DPPH, ABTS, OH, PTIO radical scavenging, and FRAP assays were used to detect the antioxidant activity of the extracellular extracts of the strains, and total phenol and total flavonoid contents of the strains with high antioxidant capacity were determined. S. aureus, B. subtilis, E. coli, and P. aeruginosa were used to determine the antibacterial activity of the mycelial extracts of the strains. Finally, the secondary metabolites of the mycelial extracts of the strains were examined by high-performance liquid chromatography. The results showed that 32 strains of Andrographis paniculata were relatively isolated > 70% and non-pathogenic. Extracellular extracts of strains AP-1 and AP-4 showed vigorous antioxidant activity, and AP-4, AP-12, AP-47, and AP-48 showed antibacterial activity against four strains of bacteria. The HPLC results indicated that the mycelial extracts of AP-4 and AP-12 contained diterpene lactones. The two endophytic fungi were recognized as Colletotrichum sp. The study successfully obtained diterpene lactones from the endophytic fungus of Andrographis paniculata and confirmed the feasibility of using endophytic fungal strains to produce active substances consistent with the host. It was also useful for exploring endophytic fungi and medicinal plants. The relationship provides theoretical guidance.

show abstract

Diversity and Bioactivity of Endophytes From Angelica sinensis in China

Cited by 12 publications

References 74 publications

The Dynamic Accumulation Rules of Chemical Components in Different Medicinal Parts of Angelica sinensis by GC-MS

The Dynamic Accumulation Rules of Chemical Components in Different Medicinal Parts of Angelica sinensis by GC-MS

Correlation in endophytic fungi community diversity and bioactive compounds of Sophora alopecuroides

A New Source of Diterpene Lactones From Andrographis paniculata (Burm. f.) Nees—Two Endophytic Fungi of Colletotrichum sp. With Antibacterial and Antioxidant Activities

Contact Info

Product

Resources

About