Diversity and antagonistic potential of marine microbes collected from south-west coast of India

Sinimol, S.; Sarika, A. R.; Nair, A. Jayakumaran

doi:10.1007/s13205-015-0318-1

Cited by 11 publications

(9 citation statements)

References 24 publications

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“…In previous studies, the best preparation of flavonoids from Malus domestica , Launaea procumbens , kumquat , and Spanish olive cultivars was obtained with the use of ethyl acetate [ 8 , 30 , 35 , 36 ]. It has been reported that ethyl acetate is the optimal reagent for isolation of active substances from plant materials [ 37 , 38 ]. In present study, it was confirmed that among all the employed organic solvent mixtures, ethyl acetate was the most effective solvent for the preparation of flavonoid-rich extracts.…”

Section: Resultsmentioning

confidence: 99%

Screening for antioxidant and antibacterial activities of phenolics from Golden Delicious apple pomace

Zhang

Wei

Zhuang

et al. 2016

Chemistry Central Journal

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BackgroundSynthetic antioxidants and antimicrobials are losing ground to their natural counterparts and therefore, the food industry has motivated to seek other natural alternatives. Apple pomace, a by-product in the processing of apples, is rich in polyphenols, and plant polyphenols have been used as food additives owing to their strong antioxidant and antimicrobial properties. The goal of this study was to screen the individual polyphenols with antioxidant and antimicrobial activities from the extracts (methanol, ethanol, acetone, ethyl acetate, and chloroform) of Golden Delicious pomace.ResultsFirst, the polyphenolic compounds (total phenol content, TPC; total flavonoids, TFD; total flavanols, TFL) and antioxidant activities (AAs) with four assays (ferric reducing antioxidant power, FRAP; 1,1-diphenyl-2-picryhydrazyl radical scavenging capacity assay, DRSC; hydroxyl radical averting capacity assay, HORAC; oxygen radical absorbance capacity assay, ORAC) were analyzed. The results showed a significant positive correlation (P < 0.05) between AAs and TFD. Ethyl acetate extract (EAE) exhibited the highest TFD with a concentration of 1.85 mg RE/g powder (expressed as rutin equivalents), and the highest AAs (expressed as butylated hydroxytoluene (BHT) equivalents) with 2.07 mg BHT/g powder for FRAP, 3.05 mg BHT/g powder for DRSC, 5.42 mg BHT/g powder for HORAC, and 8.89 mg BHT/g powder for ORAC. Composition and AA assays of individual polyphenols from the EAE were then performed. Phloridzin and phloretin accounted for 46.70 and 41.94 % of TFD, respectively. Phloretin displayed the highest AA, followed by phloridzin. Finally, the antimicrobial activities of the EAE, phloridzin, and phloretin were evaluated. EAE displayed good inhibitory activities against Staphylococcus aureus with a minimum inhibition concentration (MIC) of 1.25 mg/ml and against Escherichia coli with a MIC of 2.50 mg/ml. Phloridzin and phloretin showed better inhibitory activities than the EAE, which were MICs of 0.50 and 0.10 mg/ml, respectively, against S. aureus and MICs of 1.50 and 0.75 mg/ml, respectively, against E. coli.ConclusionsEthyl acetate was the best solvent of choice to extract natural products to obtain the maximum antioxidant and antibacterial benefits. Phloridzin and phloretin have the potential to be used as natural alternatives to synthetic antioxidants and antimicrobials.

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Section: Resultsmentioning

confidence: 99%

Screening for antioxidant and antibacterial activities of phenolics from Golden Delicious apple pomace

Zhang

Wei

Zhuang

et al. 2016

Chemistry Central Journal

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“…Antagonists have also been isolated from fruit, and these have shown potential for exploitation as BCA of postharvest diseases [14]. The marine environment is also a deposit of new bioactive compounds; thousands of unique compounds have been isolated from this environment [102,103]. In recent years, any bioactive compound and different samples from marine environments have been searched for.…”

Section: Search For New Antagonistsmentioning

confidence: 99%

Biocontrol of Postharvest Fruit Fungal Diseases by Bacterial Antagonists: A Review

et al. 2019

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This review deals with the main mechanisms of action exerted by antagonistic bacteria, such as competition for space and nutrients, suppression via siderophores, hydrolytic enzymes, antibiosis, biofilm formation, and induction of plant resistance. These mechanisms inhibit phytopathogen growth that affects postharvest fruit since quality and safety parameters are influenced by the action of these microorganisms, which cause production losses in more than 50% of fruit tree species. The use of synthetic fungicide products has been the dominant control strategy for diseases caused by fungi. However, their excessive and inappropriate use in intensive agriculture has brought about problems that have led to environmental contamination, considerable residues in agricultural products, and phytopathogen resistance. Thus, there is a need to generate alternatives that are safe, ecological, and economically viable to face this problem. Phytopathogen inhibition in fruit utilizing antagonist microorganisms has been recognized as a type of biological control (BC), which could represent a viable and environmentally safe alternative to synthetic fungicides. Despite the ecological benefit that derives from the use of controllers and biological control agents (BCA) at a commercial level, their application and efficient use has been minimal at a global level.

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“…Species of Bacillus are easy to culture and can survive under harsh conditions. Bioactive metabolite and enzyme production is common feature of Bacillus species (Mondol et al 2013;Sinimol et al 2016). In addition to Bacillus, Staphylococcus is another representative of Firmicutes in this study, and is already known to produce antimicrobial metabolites (Barbieri et al 2005;Mani et al 2016).…”

Section: Discussionmentioning

confidence: 93%

Diversity and antagonistic potential of bacteria isolated from marine grass Halodule uninervis

et al. 2018

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The aim of this study was to isolate bacteria from sea grass, Halodule uninervis collected from the coastal area of Jeddah, Saudi Arabia and to screen them for antifungal and enzymatic activities. We have isolated 162 rhizo and endophytic bacteria from soil, roots, and leaves of the sea grass. Antifungal screening of isolated bacteria revealed 19 strains (11.7%) capable to inhibit growth of four pathogenic fungi, Pythium ultimum, Phytophthora capsici, pyricularia oryzae, and Rhizoctonia solani in an in vitro assay. Taxonomic and phylogenetic analyses on the basis of 16S rRNA gene sequence revealed 97-99.9% sequence identity to recognized species. Bacillus, Staphylococcus, Jeotgalicoccus, and Planococcus, within the Phylum Firmicutes, Kocuria, Arthrobacter, Ornithinimicrobium and Corynebacterium (Actinobacteria), Sulfitobacter, Roseivivax, Ruegeria (α-Proteobacteria), Moraxella, and Vibrio (γ-Proteobacteria), were isolated. Strains belong to Phylum Firmicutes remain dominant antagonistic bacteria in this study. Further hydrolytic enzyme production was determined for these antagonistic bacteria. Our results demonstrated that the sea grass represents an important source of diverse antagonistic bacteria capable of producing antifungal metabolite.

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Diversity and antagonistic potential of marine microbes collected from south-west coast of India

Cited by 11 publications

References 24 publications

Screening for antioxidant and antibacterial activities of phenolics from Golden Delicious apple pomace

Screening for antioxidant and antibacterial activities of phenolics from Golden Delicious apple pomace

Biocontrol of Postharvest Fruit Fungal Diseases by Bacterial Antagonists: A Review

Diversity and antagonistic potential of bacteria isolated from marine grass Halodule uninervis

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