Diversity analysis reveals genetic homogeneity among Indian populations of legume pod borer, Maruca vitrata (F.)

Chatterjee, Madhurima; Yadav, Jyoti; Vennila, S.; Shashank, P. R.; Jaiswal, Nisha; Sreevathsa, Rohini; Rao, Uma

doi:10.1007/s13205-019-1850-1

Cited by 10 publications

(11 citation statements)

References 33 publications

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“…For understanding the genetic structure, analysis of molecular variance (AMOVA) and that included combined population sets from all the studied locations. The present findings are in close agreement with recent similar studies on certain lepidopteran pests from India such as Tuta absoluta (Shashank et al, 2018), Maruca vitrata (Chatterjee et al, 2019) and Pectinophora gossypiella (Naik et al, 2020), including L. orbonalis (Palraju et al, 2018, Karthika et al, 2019.…”

Section: Sequence Data Analysessupporting

confidence: 93%

“…The availability of a wide range of primers for amplification of the mitochondrial cytochrome oxidase I (COI) gene from diverse invertebrate and vertebrate phyla establish this gene as a good target for species identification in animals (Atikah et al, 2020). The COI gene has also been found to be useful in differentiating cryptic species (Shashank et al, 2018), as well as for the assessment of intraspecific diversity in insects (Chatterjee et al, 2019), due to its large size and high nucleotide substitution rate (Mandal et al, 2014). Thus, the present study was undertaken to precisely trace the genetic variability among different geographic populations of L. orbonalis from India based on cytochrome oxidase I (COI) gene.…”

Section: O N L I N E C O P Ymentioning

confidence: 99%

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Genetic variability and population structure of Leucinodes orbonalis (Guenée), a severe insect pest of brinjal in India

Padwal¹,

Chakravarty²,

Srivastava³

2022

JEB

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Aim: The present study was undertaken to provide valuable insights regarding population genetic structure of Leucinodes orbonalis from diverse agro-ecologies of India. Methodology: Molecular characterization of L. orbonalis populations collected from five major agro-climatic zones of India was carried out using mitochondrial cytochrome oxidase I (COI) gene. Collected specimens were subjected to DNA extractions, partial PCR amplification and sequencing of the target gene, and multiple sequence alignments. Results: The results showed very less diversity in the nucleotide positions of the COI sequences of 79 studied specimens, with a low number of segregating sites (30), nucleotide diversity (0.00438) and overall mean genetic distance (0.004 ± 0.001). The significant negative values of neutrality tests and unimodal mismatch distribution supported the demographic expansion theory in Indian L. orbonalis. Analysis of the molecular variance revealed that 93.13% of the genetic variation was within populations, and the variation among populations was only 6.87%. The pairwise genetic differentiation was also found to be low to moderate between most of the populations. Multiple haplotypes were recorded from all the populations, and both neighbour-joining tree as well as the haplotype network showed that clustering of the haplotypes was independent of the geographical location. Interpretation: Thus, it can be inferred that Indian populations of L. orbonalis have very low genetic variation levels concerning the COI gene. There is a possible occurrence of stable inherited gene flow among populations, thereby reducing genetic variation in India.

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Section: Sequence Data Analysessupporting

confidence: 93%

Section: O N L I N E C O P Ymentioning

confidence: 99%

Genetic variability and population structure of Leucinodes orbonalis (Guenée), a severe insect pest of brinjal in India

Padwal¹,

Chakravarty²,

Srivastava³

2022

JEB

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“…Significant and negative neutrality test indices also indicate an excess of low-frequency polymorphism and are in accordance with either purifying selections or population expansion. These results are also congruent with earlier studies on several lepidopteran pest population diversity analyses from India such as Leucinodes orbonalis (Karthika et al, 2019), Tuta absoluta (Shashank et al, 2018), Maruca vitrata (Chatterjee et al, 2019) and Pectinophora gossypiella (Naik et al, 2020), apart from H. armigera (Leite et al, 2014) in Brazil. The AMOVA analysis (Table 2) revealed that 93.51% of total genetic variation was partitioned within Indian and foreign population sets and the remaining 6.49% was partitioned among populations (F ST = 0.06494, P < 0.01).…”

Section: O N L I N E C O P ÿsupporting

confidence: 92%

Molecular characterization of intraspecific variations in Helicoverpa armigera (Hübner) populations across India

Chakravarty¹,

Padwal²,

Srivastava³

2021

JEB

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Aim: The present study was undertaken to explore the genetic diversity among Helicoverpa armigera populations from varied geographic regions of India using mitochondrial cytochrome c oxidase I (COI) gene fragments. Methodology: The larval specimens of H. armigera collected from 20 locations were subjected to DNA extraction, PCR amplification of target gene, sequencing and then multiple sequence alignments. Results: Based on COI sequence data, high levels of genetic differentiation among some H. armigera populations were detected, but divergence existing was not high enough to delineate them as separate species. The Indian population as a whole exhibited similarity with global genetic assemblage. Significant negative neutrality test indices and unimodal mismatch distribution further supported that this insect experienced a demographic expansion in the past. The phylogenetic tree and median-joining haplotype network indicated that genetic similarity was not related with geographic proximity of populations. Interpretation: Differences based on genetic analyses indicate considerable subspecific level variations among H. armigera populations of India. However, there is no existence of any unidentified cryptic species of H. armigera in the country.

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“…Larval stages of M. vitrata were collected from pigeonpea elds at ICAR-Indian Agricultural Research Institute, New Delhi and their identity was con rmed by morphological characterization (Shinde et al 2017) and use of molecular markers (Chatterjee et al 2019). Healthy second to fth instar larvae were separated and a few fth instar larvae were allowed to pupate and develop into adults (Fig.…”

Section: Insect Collectionmentioning

confidence: 99%

Amenability of Maruca vitrata (Lepidoptera: Crambidae) to gene silencing through exogenous administration and host-delivered dsRNA in pigeonpea (Cajanus cajan L.)

Chatterjee

Yadav

Rathinam

et al. 2022

Physiol Mol Biol Plants

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Insect pests are one of the major biotic stresses limiting yield in commercially important food crops. The lepidopteran polyphagous spotted pod borer, Maruca vitrata causes signi cant economic losses in legumes including pigeonpea. RNAi-based gene silencing has emerged as one of the potential biotechnological tools for crop improvement. We report in this paper, RNAi in M. vitrata through exogenous administration of dsRNA encoding three functionally important genes, Alpha-amylase (αamylase), Chymotrypsin-like serine protease (CTLP) and Tropomyosin (TPM) into the larval haemolymph and their host-delivered RNAi in pigeonpea. Signi cant decline in the expression of selected genes supported by over-expression of DICER and generation of siRNA indicated the occurrence of RNAi in the dsRNA-injected larvae. Additionally, the onset of RNAi in the herbivore was demonstrated in pigeonpea, one of the prominent hosts, by host-delivered RNAi. Transgenics in pigeonpea (cv. Pusa992), a highly recalcitrant crop, were developed through a shoot apical meristem-targeted in planta transformation strategy and evaluated. Plant level bioassays in transgenic events characterized and selected at molecular level showed mortality of M. vitrata larvae as well as reduced feeding when compared to wild type. Furthermore, molecular evidences for down regulation of target genes in the insects that fed on transgenics authenticated RNAi. Considering the variability of gene silencing in lepidopteran pests, this study provided corroborative proof for the possibility of gene silencing in M. vitrata through both the strategies.

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Diversity analysis reveals genetic homogeneity among Indian populations of legume pod borer, Maruca vitrata (F.)

Cited by 10 publications

References 33 publications

Genetic variability and population structure of Leucinodes orbonalis (Guenée), a severe insect pest of brinjal in India

Genetic variability and population structure of Leucinodes orbonalis (Guenée), a severe insect pest of brinjal in India

Molecular characterization of intraspecific variations in Helicoverpa armigera (Hübner) populations across India

Amenability of Maruca vitrata (Lepidoptera: Crambidae) to gene silencing through exogenous administration and host-delivered dsRNA in pigeonpea (Cajanus cajan L.)

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