Diversity among melon landraces (Cucumis melo L.) from Greece and their genetic relationships with other melon germplasm of diverse origins

Staub, Jack E.; López-Sesé, Ana I.; Fanourakis, Nikolaos

doi:10.1023/b:euph.0000030667.63614.bd

Cited by 72 publications

(62 citation statements)

References 18 publications

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“…Values of this parameter for ''Suski-e-Sabz'' and ''Khatouni'', as the most cultivated melon in Iran, were 0.98 and 0.99, respectively. This unexpected result is similar to the results of López-Sesé et al (2002) and Tzitzikas et al (2009) for other accessions, which were attributed to the use of similar seed among related growers or selection by farmers (Staub et al 2004). Tzitzikas et al (2009) assumed that, if out crossing occurred, farmers have made efforts to maintain the genetic originality of the accessions, probably to keep original fruit traits because of regional consumer preferences.…”

Section: Discussionsupporting

confidence: 86%

“…C. melo is, therefore, considered the most diverse species in Cucumis (Stepansky et al 1999). Genetic diversity studies have used isozymes (Staub et al 1997;Akashi et al 2002), RFLPs (Zheng et al 1999), RAPDs (Garcia et al 1998;Stepansky et al 1999;Mliki et al 2001;López-Sesé et al 2003;Staub et al 2004;Sensoy et al 2007;Tanaka et al 2007), AFLPs (GarciaMas et al 2000), ISSR and simple-sequence repeat (SSR) (Katzir et al 1996;Staub et al 2000;Daning-Poleg et al 2001;López-Sesé et al 2002;Monforte et al 2003;Nakata et al 2005;Tzitzikas et al 2009) to analyze variability and relationships among melon groups. These studies have assisted in the elucidation of intraspecific relationships in melons from different origins.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity among melon accessions from Iran and their relationships with melon germplasm of diverse origins using microsatellite markers

et al. 2013

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Melon is one of the most important horticultural crops in Iran. There are a few studies on the genetic structure of Iranian melon. A set of 18 simple sequence repeat (SSR) primer pairs were used to assess the genetic diversity in a collection of 24 melon accessions representing different botanical groups of Iranian cultivated melons (vars. inodorus, cantalupensis and dudaim), along with 28 reference accessions from diverse geographic origin. All studied SSR loci were polymorphic that confirmed their usefulness for genetic analysis of melons. A total number of 141 alleles were detected, with an average of 7.8 alleles per locus for reference genotypes and 4.38 alleles per locus for Iranian accessions. The low variability within Iranian melon accessions is reflected by the low values of the observed heterozygosity (with an average of 0.119), indicating lack of intercrossing between accessions or a high rate of self-pollination. Values of observed homozygosity for ''Suski-e-Sabz'' and ''Khatouni'', as the most cultivated melon in Iran, were 0.98 and 0.99, respectively. Cluster analysis divided Iranian accessions into two major groups. The highest level of polymorphism was detected among the dudaim group. The analysis of molecular variance indicated that the majority of variation (87 %) was due to the difference within accessions. The average pairwise genetic distance among Iranian accessions was 0.674.Our results showed a distinct separation of dudaim group from the rest of Iranian accessions, even separated two different groups of var. dudaim with different traits. There was a wide genetic distance between Honey Dew, as the most popular member of inodorus group worldwide and ''Khatouni'', a major Iranian winter melon (GD = 0.809). This genetic distance shows the importance of Iranian accessions for conservation and use in breeding programs.

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Section: Discussionsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

Genetic diversity among melon accessions from Iran and their relationships with melon germplasm of diverse origins using microsatellite markers

et al. 2013

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“…Luan et al (2008), analisando 68 acessos, com 17 primers RAPD, e obtiveram uma média de alelos por lócus de 1,9. Staub et al (2004) analisaram 17 acessos de melão, com 24 primers RAPD, e detectaram 65 alelos polimórficos, com média de 2,4 alelos por primer. Todos esses autores citados encontraram nível de polimorfismo menor que o encontrado no presente trabalho.…”

Section: Resultsunclassified

“…Luan et al (2008), também, utilizando 17 primers RAPD em 68 acessos encontraram coeficiente variando entre 50 e 90%. Staub et al (2004) analisaram 17 acessos de melão da Grécia e obtiveram um coeficiente de similaridade de 13% a 60%. Buso et al (2002), analisando 38 acessos de melão, com o emprego de 22 primers obtiveram coeficiente de similaridade de 53% a 98%, mostrando que esse estudo apresenta grande variabilidade genética, provavelmente porque se utilizaram materiais bem divergentes, pela existência de grande número de acessos de diferentes origens.…”

Section: Resultsunclassified

“…Dentre as classes de marcadores, o RAPD (Random Amplified Polymorphic DNA) tem-se destacado por ser prático, rápido, de baixo custo e por identificar alto número de lócus polimórficos nos segmentos de DNA. A caracterização molecular de acessos de germoplasma de meloeiro de várias partes do mundo, pelo uso do marcador RAPD, tem sido tema de muitos artigos publicados na literatura internacional (MLIKI et al, 2001;STAUB et al, 2004;LUAN et al, 2008). No Brasil, ainda há carência de informações moleculares nos acessos coletados em pequenas propriedades, sendo necessários esforços para se obter o referido conhecimento.…”

Section: Introductionunclassified

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Caracterização molecular de acessos de melão coletados no nordeste brasileiro

et al. 2012

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RESUMO -O meloeiro está distribuído em todo o mundo, e é a espécie que possui a maior variabilidade fenotípica do gênero, observada principalmente em seus frutos. Existe grande variabilidade que consiste em importante fonte de germoplasma para programas de melhoramento. Portanto, o conhecimento da variabilidade genética de espécies vegetais e como ela se distribui, proporciona o uso racional e sustentável dos recursos genéticos. O objetivo do presente trabalho foi realizar a caracterização molecular de acessos de meloeiro coletados no Nordeste brasileiro. Foram avaliados 40 acessos e três cultivares comerciais. A caracterização molecular foi realizada com marcadores RAPD, utilizando 18 primers, os quais geraram 139 marcas polimórficas. Com os dados de similaridade genética, foram obtidos 32 grupos, e a similaridade entre os genótipos variou de 34 a 100%. Verificou-se que os marcadores RAPD foram satisfatórios em permitir a detecção de polimorfismo entre os genótipos avaliados. Os métodos de agrupamento de Tocher e o hierárquico concordaram parcialmente. O banco de germoplasma de meloeiro da UFERSA possui alta variabilidade genética entre os acessos. Termos para indexação: Cucumis melo, germoplasma, marcador RAPD. MOLECULAR CHARACTERIZATION OF MELON ACCESSES COLLECTED IN THE BRAZILIAN NORTHEASTABSTRACT -The melon plant is distributed all over the world, and it is the species that possesses the largest phenotypic variability of the genus, observed mainly in their fruits. There is a great variability that consists of important germoplasm source for improvement programs. Therefore, the knowledge of the genetic variability of vegetable species and how it is distributed provides the rational and maintainable use of the genetic resources. The objective of the present study it was to characterize 40 accesses and three commercial cultivars of melon collected in the Brazilian Northeast by RAPD markers. Forty of the 18 RAPD primers analyzed, of which generated 139 polymorphic markers. Through of the genetic similarity data were obtained 32 groups and the similarity among the genotypes varied from 36 to 100%. It was verified that the RAPD markers were satisfactory to detect the polymorphism among the genotypes analyzed. The Tocher and hierarchical grouping methods agreed partially. The germoplasm bank of UFERSA presents high genetic variability among the accesses.

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