Diversity among Streptomyces Strains Causing Potato Scab

Abstract

Eighty Streptomyces isolates, including 35 potato scab-inducing strains and 12 reference strains of Streptomyces scabies, were physiologically characterized by a total of329 miniaturized tests. Overall similarities of all strains were determined by numerical taxonomy, with the unweighted average linkage (UPGMA) algorithm and simple matching (Ssm) and Jaccard (SJ) coefficients used as measures for similarity. Three cluster groups (A to C) were defined at a similarity level of 80.1% (Ssm); these groups contained 14 clusters and 24 unclustered strains defined at a similarity level of 86.5% (Ssm) Cluster group A contained strains phenotypically related to S. griseus or S. exfoliatus, whereas cluster group B contained strains which were phenotypically related to S. violaceus or S. rochei. The majority of the pathogenic isolates and reference strains were assigned to S. violaceus (57%) and S. griseus (22%). A DNA probe derived from the rRNA operon ofS. coelicolor IMET 40271 was used to detect restriction fragment length polymorphisms (RFLPs) among 40 pathogenic and nonpathogenic Streptomyces isolates. Southern blots revealed a high degree of diversity among the pathogenic strains tested. No significant correlation between numerical classification and RFLP grouping of Streptomyces strains could be revealed. The results obtained suggest that RFLP data are of minor importance in classification

Phenotypic, genotypic and pathogenic variation among streptomycetes implicated in common scab disease

“…diastaticus (19) group. This is partially consistent with the results of Doering-Saad et al (1992) who found that a large number of putative scab-causing strains clustered into groups including S. albidoflavus (1) and S. rochei (12). As the strains in this study were predominantly from Europe, and those analysed by Doering-Saad et al (1992) were from Israel and the USA, the mixed identity of scab strains appears to be geographically widespread.…”

“…This is partially consistent with the results of Doering-Saad et al (1992) who found that a large number of putative scab-causing strains clustered into groups including S. albidoflavus (1) and S. rochei (12). As the strains in this study were predominantly from Europe, and those analysed by Doering-Saad et al (1992) were from Israel and the USA, the mixed identity of scab strains appears to be geographically widespread. The results of this study are also consistent with those of Takeuchi et al (1996) who found that several scabcausing strains were phylogenetically distinct, based on 16S rRNA relationships.…”

“…(ATCC 49173;Lambert and Loria 1989) which had not been identified as being closely related to any of the major clusters in the Williams et al (1983a) study. More extensive phenotypic studies (Kämpfer et al 1991;Doering-Saad et al 1992) found significant diversity among scab-causing strains and assigned them to several clusters. Molecular techniques have also been applied to the classification of scab strains.…”

“…© 1998 The Society for Applied Microbiology using DNA homology (Healy and Lambert 1991), Southern blotting (Doering-Saad et al 1992), rep-PCR (Sadowsky et al 1996) and sequence analysis (Takeuchi et al 1996) have suggested genetic diversity of scab-causing strains.…”

Diversity and geographic distribution of soil streptomycetes with antagonistic potential against actinomycetoma-causing Streptomyces sudanensis in Sudan and South Sudan

“…A few species are causal agents of diseases in animals: (S. cyaneus and Streptomyces sp.) [3,4] and plants such as S. scabies, S. turgidiscabies, S. luridiscabiei, S. puniciscabiei and S. niveiscabiei [5][6][7]. Relevant to humans, two species of soil Streptomyces, S. sudanensis and S. somaliensis cause actinomycetoma [8,9].…”