Diversion of stress granules and P-bodies during viral infection

Reineke, Lucas C.; Lloyd, Richard E.

doi:10.1016/j.virol.2012.11.017

Cited by 188 publications

(189 citation statements)

References 128 publications

(181 reference statements)

Supporting

Mentioning

184

Contrasting

Order By: Relevance

“…This observation is significant as the biological activities associated with a particular RNP granule are likely determined by the proteins resident within. For example, P-bodies in certain virus-infected cells have been found to contain viral proteins and to be required for efficient virus genome replication (Reineke and Lloyd 2013). Determining the protein constituents could therefore provide insight into the physiological role of a particular granule.…”

Section: Discussionmentioning

confidence: 99%

“…As a result, the precise role of P-body granules in mRNA processing remains unclear. In mammalian cells, P-bodies have also been implicated in the microRNA-mediated inhibition of translation and in the replicative life cycle of several viruses (Liu et al 2005;Bhattacharyya et al 2006;Beckham and Parker 2008;Reineke and Lloyd 2013). Therefore, P-bodies, and perhaps other RNP granules, may be associated with different biological activities depending upon the particular proteins present in these structures.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Protein Kinases Are Associated with Multiple, Distinct Cytoplasmic Granules in Quiescent Yeast Cells

Shah¹,

Nostramo²,

Zhang³

et al. 2014

Genetics

View full text Add to dashboard Cite

The cytoplasm of the eukaryotic cell is subdivided into distinct functional domains by the presence of a variety of membrane-bound organelles. The remaining aqueous space may be further partitioned by the regulated assembly of discrete ribonucleoprotein (RNP) complexes that contain particular proteins and messenger RNAs. These RNP granules are conserved structures whose importance is highlighted by studies linking them to human disorders like amyotrophic lateral sclerosis. However, relatively little is known about the diversity, composition, and physiological roles of these cytoplasmic structures. To begin to address these issues, we examined the cytoplasmic granules formed by a key set of signaling molecules, the protein kinases of the budding yeast Saccharomyces cerevisiae. Interestingly, a significant fraction of these proteins, almost 20%, was recruited to cytoplasmic foci specifically as cells entered into the G 0 -like quiescent state, stationary phase. Colocalization studies demonstrated that these foci corresponded to eight different granules, including four that had not been reported previously. All of these granules were found to rapidly disassemble upon the resumption of growth, and the presence of each was correlated with cell viability in the quiescent cultures. Finally, this work also identified new constituents of known RNP granules, including the well-characterized processing body and stress granule. The composition of these latter structures is therefore more varied than previously thought and could be an indicator of additional biological activities being associated with these complexes. Altogether, these observations indicate that quiescent yeast cells contain multiple distinct cytoplasmic granules that may make important contributions to their long-term survival.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Protein Kinases Are Associated with Multiple, Distinct Cytoplasmic Granules in Quiescent Yeast Cells

Shah¹,

Nostramo²,

Zhang³

et al. 2014

Genetics

View full text Add to dashboard Cite

show abstract

“…(P-bodies) (26). When it was ectopically expressed, ZAP localized to cytoplasmic dot-like structures in a manner that was dependent on its CCCH-type zinc-finger domains (Fig.…”

Section: Rlrs Do Not Regulate the Antiviral Response To MLV In Primarymentioning

confidence: 96%

Zinc-finger antiviral protein mediates retinoic acid inducible gene I–like receptor-independent antiviral response to murine leukemia virus

Lee

Komano

Saitoh

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

When host cells are infected by an RNA virus, pattern-recognition receptors (PRRs) recognize the viral RNA and induce the antiviral innate immunity. Toll-like receptor 7 (TLR7) detects the genomic RNA of incoming murine leukemia virus (MLV) in endosomes and mediates the antiviral response. However, the RNA-sensing PRR that recognizes the MLV in the cytosol is not fully understood.Here, we definitively demonstrate that zinc-finger antiviral protein (ZAP) acts as a cytosolic RNA sensor, inducing the degradation of the MLV transcripts by the exosome, an RNA degradation system, on RNA granules. Although the retinoic acid inducible gene I (RIG-I)-like receptors (RLRs) RIG-I and melanoma differentiation-associated protein 5 detect various RNA viruses in the cytosol and induce the type I IFN-dependent antiviral response, RLR loss does not alter the replication efficiency of MLV. In sharp contrast, the loss of ZAP greatly enhances the replication efficiency of MLV. ZAP localizes to RNA granules, where the processing-body and stress-granule proteins assemble. ZAP induces the recruitment of the MLV transcripts and exosome components to the RNA granules. The CCCH-type zincfinger domains of ZAP, which are RNA-binding motifs, mediate its localization to RNA granules and MLV transcripts degradation by the exosome. Although ZAP was known as a regulator of RIG-I signaling in a human cell line, ZAP deficiency does not affect the RIG-I-dependent production of type I IFN in mouse cells. Thus, ZAP is a unique member of the cytosolic RNA-sensing PRR family that targets and eliminates intracellular RNA viruses independently of TLR and RLR family members.host defense | retrovirus | ZC3HAV1

show abstract

“…60 Similarly, several viruses have been shown to accelerate the decay of PB resident proteins, or recruit PB resident proteins to viral replication compartments to enhance replication. 63 We are actively investigating whether individual PB resident proteins are degraded or modified to alter the structural scaffolds required for PB assembly.…”

Section: Modelmentioning

confidence: 99%

Viral activation of stress-regulated Rho-GTPase signaling pathway disrupts sites of mRNA degradation to influence cellular gene expression

Corcoran

McCormick

2015

Small GTPases

View full text Add to dashboard Cite

Diversion of stress granules and P-bodies during viral infection

Cited by 188 publications

References 128 publications

Protein Kinases Are Associated with Multiple, Distinct Cytoplasmic Granules in Quiescent Yeast Cells

Protein Kinases Are Associated with Multiple, Distinct Cytoplasmic Granules in Quiescent Yeast Cells

Zinc-finger antiviral protein mediates retinoic acid inducible gene I–like receptor-independent antiviral response to murine leukemia virus

Viral activation of stress-regulated Rho-GTPase signaling pathway disrupts sites of mRNA degradation to influence cellular gene expression

Contact Info

Product

Resources

About