Diversified expression patterns of <i>autotaxin</i>, a gene for phospholipid‐generating enzyme during mouse and chicken development

Ohuchi, Hideyo; Hayashibara, Yasunori; Matsuda, Hironao; Onoi, Motoyoshi; Mitsumori, Masayuki; Tanaka, Masayuki; Aoki, Junken; Arai, Hiroyuki; Noji, Sumihare

doi:10.1002/dvdy.21158

Cited by 5 publications

(7 citation statements)

References 27 publications

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“…The ATX catalytic activity [66][67][68] is implicated in the early stages of embryo development. A minute description of the ATX expression pattern during mouse development has also been reported [67].…”

Section: Autotaxin and Physio-pathologymentioning

confidence: 99%

Autotaxin

Boutin

Ferry

2009

Cell. Mol. Life Sci.

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Autotaxin is a protein of approximately 900 amino acids discovered in the early 1990s. Over the past 15 years, a strong association between cancer cells and autotaxin production has been observed. Recent publications indicate that autotaxin and the capacity of cancer to metastasise are intimately linked. The discovery of new molecular targets in pharmacology is a mixture of pure luck, hard work and industrial strategy. Despite a crucial and desperate need for new therapeutic tools, many targets are approached in oncology, but only a few are validated and end up at the patient bed. Outside the busy domain of kinases, few targets have been discovered that can be useful in treating cancer, particularly metastatic processes. The fortuitous relationship between autotaxin and lysophosphatidic acid renders the results of observations made in the diabetes/obesity context considerably important. The literature provides observations that may aid in redesigning experiments to validate autotaxin as a potential oncology target.

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Section: Autotaxin and Physio-pathologymentioning

confidence: 99%

Autotaxin

Boutin

Ferry

2009

Cell. Mol. Life Sci.

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“…Compatible with our findings, Koike et al reported that, using ex vivo whole embryo culture, Ki16425 induced defects in blood vessel formation of the mouse yolk sac, like ATX-defective embryos of mice, due to the impaired formation of distinctive large lysosomes in yolk sac visceral endoderm cells through the lysoPtdOH receptor-G 12/13 -Rho-ROCK-LIM kinase pathway [29,33]. Many similarities for the development of extra-embryonic membranes and the fundamental body were observed in all amniotes including mouse and chicken [10,34], and thus, the same events may occur in the yolk sac of the hypoblast (visceral endoderm in mice). However, it is not clear how lysoPtdOH gains access to the hypoblast in the yolk sac cavity through the epiblast on the yolk sac surface.…”

Section: Discussionmentioning

confidence: 99%

“…Although these results suggested that lysoPtdOH is an angiogenic molecule in mouse embryonic development, mechanistic information on how lysoPtdOH induces angiogenesis is still lacking. Similar approaches were made to assess the effects of lysoPtdOH production by ATX on chicken embryonic development [10]. There is an interesting study with chicken eggs showing that lysoPtdOH is involved in the blood vessel network formation on the chicken chorioallantoic membrane (CAM) [11].…”

mentioning

confidence: 99%

Lysophosphatidic Acid Produced by Hen Egg White Lysophospholipase D Induces Vascular Development on Extraembryonic Membranes

Morishige

Uto

Hori

et al. 2013

Lipids

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Lysophosphatidic acid (lysoPtdOH), a lysophospholipid mediator, exerts diverse physiological effects, including angiogenesis, through its specific G-protein-coupled receptors. Previously, we showed that unfertilized hen egg white contains polyunsaturated fatty acid-rich lysoPtdOH and lysophospholipase D (lysoPLD). Here, we examined whether lysoPtdOH was produced by lysoPLD in the presence and absence of a hen fertilized ovum and what the physiological role of lysoPtdOH in hen egg white is. Mass spectrometry showed that fertilized hen egg white contained about 8 μM lysoPtdOH before incubation with an ovum, mainly comprised of 18:1- (12.6 %), 18:2- (37.8 %) and 20:4-molecular species (41.5 %). In an early gestation period, the lysoPtdOH was increased up to 9.6 μM, concomitant with a decrease in the level of polyunsaturated lysophosphatidylcholine (lysoPtdCho). Moreover, lysoPtdOH-degrading activities were found in egg white and the vitelline membrane, showing that these enzymes control lysoPtdOH levels in egg white. In an egg yolk angiogenesis assay, two lysoPtdOH receptor antagonists, Ki16425 and N-palmitoyl serine phosphoric acid (NASP), inhibited blood vessel formation induced by exogenously added 18:1-lysoPtdOH and its precursor lysoPtdCho on the hen yolk sac. Ki16425 and NASP also inhibited blood vessel formation in the chorioallantoic membrane (CAM). Furthermore, the relatively higher levels of LPA₁, LPA₂, LPA₄ and LPA₆ mRNA were present in the yolk sac and CAM. These results suggest that lysoPtdOH produced from lysoPtdCho by the action of lysoPLD in hen egg white is involved in the formation of blood vessel networks through several lysoPtdOH receptors on various extraembryonic membranes, including the yolk sac membrane and CAM.

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“…Antisense and sense (control) riboprobes for each gene were prepared using a digoxygenin (DIG) RNA labeling kit (Roche) using appropriate cDNA fragments. E11.5 embryos of ICR mice were dissected from female mice, embedded in OCT compound 2A and E), particularly in the posterior regions of the vertebrae that overlap with the expression domain of ATX [6,7]. In addition to these sites, LPA 1 transcripts were evident in the medial nasal process, the mandibular compartment of first and second branchial arches, and other structures ( Fig.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

“…Subsequent overt chondrogenesis provides a cartilaginous mold for bone formation in mammals [1][2][3]. It has been shown that autotaxin (ATX), a tumor cell motility-stimulating factor [4,5], is abundantly expressed in the condensing mesenchyme of the future vertebra and also in the epiphyseal region of the developing limb in mouse and chick embryos [6,7]. This suggests a physiological role of lysophosphatidic acid (LPA) at these sites during skeletal development, since ATX has been identified as a secreted lysophospholipase D and plays a principal role in LPA production in the body [8,9].…”

Section: Introductionmentioning

confidence: 99%

Stimulatory actions of lysophosphatidic acid on mouse ATDC5 chondroprogenitor cells

et al. 2010

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Lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are bioactive lysophospholipids that affect various cellular processes through G protein-coupled receptors. In our current study, we found by in situ hybridization that E11.5 mouse embryos strongly expressed the LPA receptor subtype LPA(1) in cartilaginous bone primordia and the surrounding mesenchymal cells. However, despite their wide-ranging actions, the roles of lysophospholipids in chondrogenesis remain poorly understood. The mouse clonal cell line ATDC5 undergoes a sequential differentiation of chondroprogenitor cells in vitro. Undifferentiated and differentiated ATDC5 cells express LPA(1) and other lysophospholipid receptors including S1P receptor S1P(1) and S1P(2). Taking advantage of this cell model, we studied the effects of LPA on the activities of chondroprogenitor cells. LPA markedly stimulates both DNA synthesis and the migration of ATDC5 chondroprogenitor cells in culture, whereas S1P suppresses the migration of these cells. Treatment with Ki16425, an LPA(1)- and LPA(3)-specific receptor antagonist, suppressed the fetal bovine serum-stimulated migration of ATDC5 cells by almost 80%. These results indicate that LPA plays an important role in the activation of chondroprogenitor cells.

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Diversified expression patterns of autotaxin, a gene for phospholipid‐generating enzyme during mouse and chicken development

Cited by 5 publications

References 27 publications

Autotaxin

Autotaxin

Lysophosphatidic Acid Produced by Hen Egg White Lysophospholipase D Induces Vascular Development on Extraembryonic Membranes

Stimulatory actions of lysophosphatidic acid on mouse ATDC5 chondroprogenitor cells

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