Diversification of the P genome among Agropyron Gaertn. (Poaceae) species detected by FISH

Zhao, Yan; Xie, Jihong; Dou, Quanwen; Wang, Junjie; Zhang, Zhong

doi:10.3897/compcytogen.v11i3.13124

Cited by 8 publications

(6 citation statements)

References 32 publications

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“…Most of these species showed quite stable 1C DNA content values across the transect, except Artemisia frigida and Agropyro cristatum . Previous studies had reported the 1C value for diploid A. frigida(2.63 pg) and A. cristatum (6.49 pg) (Garcia et al 2008;Said et al 2018), with their polyploid species being identified in nature (Wan et al 2011;Zhao et al 2017). In our case, the measured 1C values indicated that A. frigida andA.…”

Section: Plant Genome Size Measurementssupporting

confidence: 59%

β-diversity in temperate grasslands is driven by stronger environmental filtering of plant species with large genomes

Zhang

Lü

Wei

et al. 2021

Preprint

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Elucidating mechanisms underlying community assembly and biodiversity patterns is central to ecology and evolution. Genome size (GS, i.e. nuclear DNA content) determines species’ capacity to tolerate environmental stress and therefore potentially drives community assembly. However, its role in driving β-diversity (i.e., spatial variability in species composition) remains unclear. We measured GS for 161 plant species and investigated their occurrences within plant communities across 52 sites spanning a 3200-km transect in the temperate grasslands of China. Using species distribution modelling, we found that environmental factors showed larger effects on β-diversity of large-GS than that of small-GS species and that communities with abundant resources had a greater representation of large-GS species. The latter finding was confirmed following analysis of data from a 10-yr resource (water, nitrogen, and phosphorus) manipulation experiment in which resource addition resulted in increased community weighted GS based on plant biomass estimates, suggesting that large-GS species are more sensitive to environmental resource limitation and explaining the greater environmental selection on β-diversity of large-GS species. These findings highlight the roles of GS in driving community assembly and predicting species responses to global change.

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Section: Plant Genome Size Measurementssupporting

confidence: 59%

β-diversity in temperate grasslands is driven by stronger environmental filtering of plant species with large genomes

Zhang

Lü

Wei

et al. 2021

Preprint

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“…The nature of the polyploid genome of A. cristatum and the origin of its P genome(s) have not yet been clarified. Schulz-Schaeffer et al (1963) proposed the segmental allopolyploid nature of tetraploid and hexaploid A. cristatum , while other authors consider the tetraploid A. cristatum as autopolyploid, originating from the diploid A. cristatum (Taylor and McCoy 1973; Vogel et al 1999; Zhao et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers

et al. 2019

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Key messageUsing COS markers, the study reveals homeologous relationships between tetraploid Agropyron cristatum and bread wheat to support alien introgression breeding of wheat.AbstractCrested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat that possesses many genes that are potentially useful in wheat improvement. The species comprises a complex of diploid, tetraploid and hexaploid forms. In this study, wheat–A. cristatum chromosome, telosome and translocation lines were used to characterize syntenic relationships between tetraploid A. cristatum and bread wheat. Prior to mapping COS markers, the cytogenetic stock lines were characterized for fertility and by FISH and GISH for karyotype stability. Out of 328 COS markers selected for the study, 279 consistently amplified products in tetraploid A. cristatum, and, out of these, 139 were polymorphic between tetraploid crested wheatgrass and wheat. Sixty-nine markers were found to be suitable for the detection of tetraploid A. cristatum chromosomes 1P–6P in wheat, ranging from 6 to 17 markers per chromosome. BLASTn of the source ESTs resulted in significant hits for 67 markers on the wheat pseudomolecules. Generally, COS markers of the same homeologous group were detected on similar arms in both Agropyron and wheat. However, some intragenomic duplications and chromosome rearrangements were detected in tetraploid A. cristatum. These results provide new insights into the structure and evolution of the tetraploid A. cristatum genome and will facilitate the exploitation of the wild species for introgression breeding of bread wheat.Electronic supplementary materialThe online version of this article (10.1007/s00122-019-03394-1) contains supplementary material, which is available to authorized users.

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“…Agropyron species are considered as highly valuable sources to improve the crop resistance to biotic and abiotic stresses as well as quality and yield‐related traits (Ceoloni et al, 2015). One of the most widely spread species is crested wheatgrass, which is distributed in Eurasia (Dewey, 1984; Yang et al, 2014; Zhao et al, 2017). The species, and the tetraploid form in particular, also referred to as desert crested wheatgrass [ A. desertorum (Fisch.…”

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confidence: 99%

Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting

et al. 2019

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Core Ideas Flow sorting enabled to dissect the genome of crested wheatgrass into chromosomes. This is a powerful approach to access the complex genome of a wheat wild relative. Chromosome genomics will support alien introgression breeding of the crop. Wheatgrass (Agropyron sp.) is a potential source of beneficial traits for wheat improvement. Among them, crested wheatgrass [A. cristatum (L.) Gaertn.] comprises a complex of diploid, tetraploid, and hexaploid forms with the basic genome P, with some accessions carrying supernumerary B chromosomes (Bs). In this work, we applied flow cytometry to dissect the complex genome of crested wheatgrass into individual chromosomes to facilitate its analysis. Flow karyotypes obtained after the analysis of 4ʹ,6‐diamidino‐2‐phenylindole (DAPI)‐stained mitotic chromosomes of diploid and tetraploid accessions consisted of three peaks, each corresponding to a group of two or three chromosomes. To improve the resolution, bivariate flow karyotyping after fluorescent labeling of chromosomes with fluorescein isothiocyanate (FITC)‐conjugated probe (GAA)7 microsatellite was applied and allowed discrimination and sorting of P genome chromosomes from wheat–crested wheatgrass addition lines. Chromosomes 1P–6P and seven telomeric chromosomes could be sorted at purities ranging from 81.7 to 98.2% in disomics and from 44.8 to 87.3% in telosomics. Chromosome 7P was sorted at purities reaching 50.0 and 39.5% in diploid and tetraploid crested wheatgrass, respectively. In addition to the whole complement chromosomes (A), Bs could be easily discriminated and sorted from a diploid accession at 95.4% purity. The sorted chromosomes will streamline genome analysis of crested wheatgrass, facilitating gene cloning and development of molecular tools to support alien introgression into wheat.

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Diversification of the P genome among Agropyron Gaertn. (Poaceae) species detected by FISH

Cited by 8 publications

References 32 publications

β-diversity in temperate grasslands is driven by stronger environmental filtering of plant species with large genomes

β-diversity in temperate grasslands is driven by stronger environmental filtering of plant species with large genomes

Uncovering homeologous relationships between tetraploid Agropyron cristatum and bread wheat genomes using COS markers

Dissecting the Complex Genome of Crested Wheatgrass by Chromosome Flow Sorting

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