Abstract:Resumo -O objetivo deste trabalho foi avaliar a diversidade genética, entre e dentro de progênies de dendezeiro tipo dura, de origem Deli. A caracterização genética foi feita com uso de marcadores microssatélites em 24 progênies usadas na produção comercial de sementes, sendo 22 provenientes de autofecundação e duas de cruzamentos entre irmãos completos. Foi realizada análise de variância molecular entre e dentro das progênies, com posterior construção de um dendrograma. Observou-se baixa variabilidade genétic… Show more
“…The AMOVA results showed that 14% of variance occurred among offspring and 86% occurred within offspring ( Table 2). These results corroborate the expected variance in allogamous species, where crossing occurring naturally between parents and the variation within offspring are low (Glémin et al, 2006;Ferreira et al, 2012). Glémin et al (2006) showed that among various life history traits, mating systems have the greatest influence on patterns of polymorphism.…”
Section: Molecular Markers and Genetic Diversitysupporting
Napier grass [Cenchrus purpureus (Schumach.) Morrone], also known as elephant grass, is an important tropical forage that has the potential to become an alternative feedstock for energy production. Knowledge about the mode of reproduction and outcrossing rate is essential to select the best strategy to apply in breeding programs to develop new cultivars for application in energy and forage production systems. The objective of this work was to evaluate the outcrossing rate in two Napier grass breeding populations. Six microsatellite markers were used to genotype 28 half‐sibling families totaling 588 individuals, and pollen viability was checked by fluorescein diacetate test (FDA) methodology. A total of 14,603 pollen grains were assessed; 7751 (53%) were considered viable, and 6852 (47%) were considered sterile. Forty‐two alleles were found among all evaluated individuals, and analysis of molecular variance results showed that 14% of variance occurred among half‐siblings and 86% occurred within half‐siblings. Comparison of molecular data among parental and half‐sibling populations found 95.3% of seeds derived from outcrossing, indicating that Napier grass is predominantly allogamous. Currently, directional crosses and hybrid formation in Napier grass depend on the inflorescence protection and pollen collection of selected individuals. Since our results suggest that the self‐fecundation rate is very low (5%), other strategies like unprotected directional crosses can be applied to implementation of large‐scale hybrid production.
“…The AMOVA results showed that 14% of variance occurred among offspring and 86% occurred within offspring ( Table 2). These results corroborate the expected variance in allogamous species, where crossing occurring naturally between parents and the variation within offspring are low (Glémin et al, 2006;Ferreira et al, 2012). Glémin et al (2006) showed that among various life history traits, mating systems have the greatest influence on patterns of polymorphism.…”
Section: Molecular Markers and Genetic Diversitysupporting
Napier grass [Cenchrus purpureus (Schumach.) Morrone], also known as elephant grass, is an important tropical forage that has the potential to become an alternative feedstock for energy production. Knowledge about the mode of reproduction and outcrossing rate is essential to select the best strategy to apply in breeding programs to develop new cultivars for application in energy and forage production systems. The objective of this work was to evaluate the outcrossing rate in two Napier grass breeding populations. Six microsatellite markers were used to genotype 28 half‐sibling families totaling 588 individuals, and pollen viability was checked by fluorescein diacetate test (FDA) methodology. A total of 14,603 pollen grains were assessed; 7751 (53%) were considered viable, and 6852 (47%) were considered sterile. Forty‐two alleles were found among all evaluated individuals, and analysis of molecular variance results showed that 14% of variance occurred among half‐siblings and 86% occurred within half‐siblings. Comparison of molecular data among parental and half‐sibling populations found 95.3% of seeds derived from outcrossing, indicating that Napier grass is predominantly allogamous. Currently, directional crosses and hybrid formation in Napier grass depend on the inflorescence protection and pollen collection of selected individuals. Since our results suggest that the self‐fecundation rate is very low (5%), other strategies like unprotected directional crosses can be applied to implementation of large‐scale hybrid production.
“…The analysis also showed that 42.84 % of the genetic variation was found among individuals within families. This shows that the species is preferably cross-fertilized, since allogamous species retain much of their genetic variability distributed within their families, in contrast to self-pollinating species, where most of the genetic variability is between provenances (Ferreira et al 2012).…”
Section: Resultsmentioning
confidence: 99%
“…Identifying genetically superior progenies that may be recommended for use in seed production would be an important step for improving the crop in Brazil (Valente et al 2017a). However, previous studies are necessary to provide a basis for strategies to make the best of the available genetic resources and to maintain a broad genetic base within breeding programs (Ferreira et al 2012). Genetic markers are a highly efficient tool for investigating the variability between and within progenies.…”
Section: Abstract Resumomentioning
confidence: 99%
“…The data obtained may help making important decisions, regarding the management and conservation of plant resources. For instance, in reciprocal recurrent selection programs, the knowledge of genetic diversity between and within progenies may speed up the development of new varieties (Ferreira et al 2012).…”
Sacha inchi (Plukenetia volubilis L.) is a species with nutraceutical benefits traditionally consumed by Amazonic indigenous and urban communities. Studies on the genetic diversity and mating system are required to preserve and make the best use of the genetic resources for this species. This study aimed to estimate the genetic diversity and mating system parameters of sacha inchi progenies using Amplified Fragment Length Polymorphism (AFLP) markers. A total of 360 progenies from 30 accessions of sacha inchi were analyzed using three AFLP primers combinations. The percentage variation between and within families and the crossing rate, among other parameters, were estimated. The AFLP markers were efficient in genetically differentiating progenies, revealing 251 (98.82 %) polymorphic fragments. The analysis of molecular variance showed that the strongest variation occurs between progenies (57.16 %). However, the genetic differentiation within progenies was considerable (42.84 %), and could be exploited in breeding programs. The estimated population outcrossing rate was high (0.957), indicating it is a predominantly allogamous species. On the other hand, 33.7 % of the crosses occurred between related individuals. The estimate for biparental crosses revealed that the progenies consisted mainly of half-siblings (66.9 %) and full-siblings (28.8 %). For purposes of breeding and ex situ genetic conservation, sacha inchi seeds should be collected from a large number of parent plants.
“…Its economic prominence is due to its oil, being one of the world's leading sources of vegetable oil. (FERREIRA et al, 2012;USDA, 2012). E. guineensis oil can be extracted from both the fruit (palm oil) and the nut (palm kernel oil).…”
This study aimed to estimate the genetic diversity of three Elaeis guineensis populations in Rio Grande do Norte state, as well as to verify the decreases in effective population size. The population with the highest polymorphism was MAT (Mata) with+ 57 loci (72%), followed by RIA (Riacho) with 54 loci (68%) and HOR (Horta) with 34 loci (43.03%). The RIA population was shown to be the most genetically diverse, with Nei (h = 0.28) and Shannon (I = 0.41) diversity indices. There was high genetic differentiation among populations (AMOVA, analysis of molecular variance = 42%), which was separated into three distinct genetic groups according to a Bayesian analysis. There was a significant population decrease (P < 0.05) for the HOR population in the IAM (infinite allele model) and SMM (stepwise mutation model), and for the RIA population in the IAM. The data obtained in this study may support ex-situ conservation projects for Elaeis guineensis, contributing to the selection of genotypes and their sustainable use.
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