Diversidade genética de Xylella fastidiosa em regiões produtoras de citros na Bahia

Casais, Vinícius; Patrocínio, Epaminondas do; Oliveira, S. A. S. de; Schnadelbach, Alessandra Selbach; Barbosa, C. de J.; Barbosa, Luciana Veiga

doi:10.1590/s0100-204x2014000100004

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…The two most expensive activities were the cheapest when comparing monitored and unmonitored teams, which corroborates the importance of the monitoring system. Casais et al (2014) state that huanglongbing (HLB), or citrus greening, has been considered the worst and most destructive disease affecting commercial citrus groves worldwide. In view of this reality, psyllid control alone represents 55% of the total cost of spraying, with preventive expenses being more pertinent than the risk of the biological and financial damages that may be caused.…”

Section: Resultsmentioning

confidence: 99%

Economic viability of a monitoring system in mechanized citrus production

Silva¹,

Santos²,

Vidal³

et al. 2020

Científica

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Digital agriculture is currently the main technological paradigm in agribusiness, in which one of the main challenges is the convergence of new monitoring technologies with the management of the volume of information generated to increase productivity. This research analyzes the economic viability of a monitoring system deployed on eight tractors and two trucks used for spraying operations in a citrus farm. The operations covered an area of 2,236 ha and were performed during the 2016/2017 harvest. The economic results of the investment were examined using the discounted cash flow method associated with the Monte Carlo simulation. The monitoring system provided greater availability and quality of information, which improved the quality of operations management. The results pointed to 23.67% lower costs per hectare in monitored compared to unmonitored areas. Economic analysis demonstrated the viability of the investment with 99.5% confidence, whose gain in Net Present Value per machine represents 15% of the machine acquisition cost. The results of this study are pioneering for mechanized citrus operations and denote the importance of investments in automated digital monitoring systems that allow better monitoring of management activities in orange production.

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Section: Resultsmentioning

confidence: 99%

Economic viability of a monitoring system in mechanized citrus production

Silva¹,

Santos²,

Vidal³

et al. 2020

Científica

View full text Add to dashboard Cite

show abstract

“…Sweet oranges are the most susceptible, while grapefruit, mandarins, lemons, limes and trifoliate orange are only moderately susceptible (Garcia et al, 2012; Gmitter et al, 2012; Casais et al, 2014; Fadel et al, 2014). In most cases CVC is not lethal but infected trees always display a reduced vigor and growth rate and a decreased productive lifespan.…”

Section: Hosts and Diseasesmentioning

confidence: 99%

Xylella fastidiosa: Host Range and Advance in Molecular Identification Techniques

Baldi

Porta

2017

Front. Plant Sci.

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In the never ending struggle against plant pathogenic bacteria, a major goal is the early identification and classification of infecting microorganisms. Xylella fastidiosa, a Gram-negative bacterium belonging to the family Xanthmonadaceae, is no exception as this pathogen showed a broad range of vectors and host plants, many of which may carry the pathogen for a long time without showing any symptom. Till the last years, most of the diseases caused by X. fastidiosa have been reported from North and South America, but recently a widespread infection of olive quick decline syndrome caused by this fastidious pathogen appeared in Apulia (south-eastern Italy), and several cases of X. fastidiosa infection have been reported in other European Countries. At least five different subspecies of X. fastidiosa have been reported and classified: fastidiosa, multiplex, pauca, sandyi, and tashke. A sixth subspecies (morus) has been recently proposed. Therefore, it is vital to develop fast and reliable methods that allow the pathogen detection during the very early stages of infection, in order to prevent further spreading of this dangerous bacterium. To this purpose, the classical immunological methods such as ELISA and immunofluorescence are not always sensitive enough. However, PCR-based methods exploiting specific primers for the amplification of target regions of genomic DNA have been developed and are becoming a powerful tool for the detection and identification of many species of bacteria. The aim of this review is to illustrate the application of the most commonly used PCR approaches to X. fastidiosa study, ranging from classical PCR, to several PCR-based detection methods: random amplified polymorphic DNA (RAPD), quantitative real-time PCR (qRT-PCR), nested-PCR (N-PCR), immunocapture PCR (IC-PCR), short sequence repeats (SSRs, also called VNTR), single nucleotide polymorphisms (SNPs) and multilocus sequence typing (MLST). Amplification and sequence analysis of specific targets is also mentioned. The fast progresses achieved during the last years in the DNA-based classification of this pathogen are described and discussed and specific primers designed for the different methods are listed, in order to provide a concise and useful tool to all the researchers working in the field.

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Diversidade genética de Xylella fastidiosa em regiões produtoras de citros na Bahia

Cited by 2 publications

References 19 publications

Economic viability of a monitoring system in mechanized citrus production

Economic viability of a monitoring system in mechanized citrus production

Xylella fastidiosa: Host Range and Advance in Molecular Identification Techniques

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