Diverse types of epineural conduits for bridging short nerve defects. An experimental study in the rabbit

Ignatiadis, Ioannis; Yiannakopoulos, Christos K.; Barbitsioti, Antonia D.; Avram, A; Patralexis, Haralambos G.; Tsolakis, Charilaos; Papalois, Apostolos; Xenakis, Theodore; Beris, Alexandros E.; Soucacos, P.N.

doi:10.1002/micr.20313

Cited by 22 publications

(26 citation statements)

References 21 publications

(13 reference statements)

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“…Our laboratory has previously established a rabbit sciatic nerve model for studying nerve regeneration through an epineural flap conduit across a short nerve gap (10 mm). 21 According to this study, nerve regeneration through the epineural conduits was comparable with those provided by nerve grafting. Despite favoring results of this study, 21 the challenge of accelerating nerve regeneration still exists.…”

mentioning

confidence: 55%

“…21 According to this study, nerve regeneration through the epineural conduits was comparable with those provided by nerve grafting. Despite favoring results of this study, 21 the challenge of accelerating nerve regeneration still exists. The purpose of this experimental project was to study the effect of direct administration of NGF into an epineural conduit across a short nerve gap (10 mm) in a rabbit sciatic nerve model.…”

mentioning

confidence: 55%

“…Based on the results of the above-mentioned study, 21 the data of nerve regeneration through the epineural conduits were comparable with those provided by nerve grafting. In this study, we only focused on evaluation of the effect of direct administration of NGF to an epineural conduit on nerve regeneration.…”

Section: Discussionmentioning

confidence: 76%

“…In contrast to the above-mentioned studies, 8,9,23,24 our laboratory has selected the rabbit sciatic nerve transection model, because it has a well-developed epineurium, which can be harvested without significantly injuring the nerve. 21 Moreover, the complications of automutilation and autotomy seen with the rat sciatic nerve injury model are completely avoided. 25 The use of epineural conduits has several advantages: 1) there is no need to harvest a donor nerve; 2) avoidance of donor-site morbidity (e.g., painful neuroma formation, loss of sensibility); 3) a neural origin conduit is used; 4) there is no donor-recipient size mismatch; 5) the biocompatibility is perfect; 6) there is no antigenicity or inflammatory reaction; and 7) the cost of harvesting is negligible.…”

Section: Discussionmentioning

confidence: 99%

“…Our Department 21 has evaluated the efficacy of epineural conduits in nerve regeneration using the rabbit sciatic nerve model. In our previous study, three variations of an epineural flap were used to bridge a 10-mm nerve defect in the rabbits' sciatic nerve.…”

Section: Discussionmentioning

confidence: 99%

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Nerve growth factor combined with an epineural conduit for bridging a short nerve gap (10 mm). A study in rabbits

Barmpitsioti¹,

Konofaos

Ignatiadis

et al. 2011

Microsurgery

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The purpose of this study was to evaluate the effect of direct administration of nerve growth factor (NGF) into an epineural conduit across a short nerve gap (10 mm) in a rabbit sciatic nerve model. The animals were divided into two groups. In group 1, n = 6, a 10-mm defect was created in the sciatic nerve and bridged with an epineural flap. A dose of 1 μg of NGF was locally administered daily for the first 21 days. NGF administration was made inside the epineural flap using a silicone reservoir connected to a silicone tube. In group 2, n = 6, the 10-mm defect was bridged with a nerve graft. This group did not receive any further treatment. At 13 weeks, all animals, before euthanasia, underwent electromyography (EMG) studies and then specimen sent for histology morphometric analysis. NGF administration ensured a significantly increased average number of myelinated axons per μm(2) (P = 0.028) and promoted fiber maturation (P = 0.031) and better EMG results (P = 0.046 for latency P = 0.048 for amplitude), compared with the control group. Although nerve grafts remain the gold standard for peripheral nerve repair, NGF-treated epineural conduits represent a good alternative, particularly when an unfavorable environment for nerve grafts is present.

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mentioning

confidence: 55%

mentioning

confidence: 55%

Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Nerve growth factor combined with an epineural conduit for bridging a short nerve gap (10 mm). A study in rabbits

Barmpitsioti¹,

Konofaos

Ignatiadis

et al. 2011

Microsurgery

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PDLLA/PRGD/β‐TCP conduits build the neurotrophin‐rich microenvironment suppressing the oxidative stress and promoting the sciatic nerve regeneration

Qiu

Yin

et al. 2014

J Biomedical Materials Res

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A novel nerve guidance conduit comprising poly{(lactic acid)-co-[(glycolic acid)-alt-(l-lysine)]} (PRGD), poly (d,l-lactic acid) (PDLLA) and β-tricalcium phosphate (β-TCP) was constructed to facilitate the peripheral nerve regeneration. From the comparative study, PDLLA/PRGD/β-TCP conduit achieved the best recovery in regard of the ultrastructure observation and the SFI evaluation. At the first stage of the injury (7 days), the maximum expression augments in ZnSOD (6.4 folds) and GPX4 (6.8 folds) were observed in PDLLA/PRGD/β-TCP group; while striking rise in actin (6.8 folds), tubulin (5.6 folds), and ERM components expressions were observed later (35 days). Meanwhile, compared with PDLLA and PDLLA/PRGD conduits, PDLLA/PRGD/β-TCP conduits achieved the highest local nerve growth factor (NGF) content and an accumulating BDNF content. We speculated that addition of RGD and β-TCP in the composites were the main positive factors to build the microenvironment rich in NGF and BDNF, which help to counteract with the oxidative stress and to boost the cytoskeletal protein expressions. Therefore, PDLLA/PRGD/β-TCP could be promising composites used in peripheral nerve regeneration.

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Posttraumatic neuroma of the radial nerve treated with an autogenous epineural conduit technique. A case report

et al. 2009

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We present the outcome of the first clinical application of a new technique using an epineural flap to bridge a short nerve defect. A 28-year-old male had suffered a radial nerve laceration at the lower third of the arm, proximal to the brachioradialis branch, 3 weeks before surgery. During surgery, a neuroma-in-continuity was excised preserving the epineural sleeve. Two longitudinal epineural flaps were created, one from the proximal and one from the distal nerve stump and used to bridge a 1-cm-long nerve defect. Each epineurium flap was sutured to the intact epineurium of the other side and additionally to each other. An electromagnetic nerve stimulator was used to enhance the nerve regeneration process. Nerve regeneration was followed up for 17 months with excellent functional results.

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Diverse types of epineural conduits for bridging short nerve defects. An experimental study in the rabbit

Cited by 22 publications

References 21 publications

Nerve growth factor combined with an epineural conduit for bridging a short nerve gap (10 mm). A study in rabbits

Nerve growth factor combined with an epineural conduit for bridging a short nerve gap (10 mm). A study in rabbits

PDLLA/PRGD/β‐TCP conduits build the neurotrophin‐rich microenvironment suppressing the oxidative stress and promoting the sciatic nerve regeneration

Posttraumatic neuroma of the radial nerve treated with an autogenous epineural conduit technique. A case report

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